Screening 5-lipoxygenase inhibitors from selected traditional Chinese medicines and isolation of the active compounds from Polygoni Cuspidati Rhizoma by an on-line bioactivity evaluation system.

To identify natural products as new prototypes for 5-lipoxygenase (5-LOX), 12 traditional Chinese medicines (TCMs) were selected for screening their 5-LOX inhibition activities. The results showed that the methanol extracts of all selected TCMs (n = 12) possessed inhibitory activities against 5-LOX at 200 μg/mL, of which six extracts of the TCMs showed significant inhibitory effects with IC values in the range from 33.2 ± 1.

Quality markers of animal medicinal materials: Correlative analysis of musk reveals distinct metabolic changes induced by multiple factors.

Background: Common farming environmental elements, such as longitude, latitude, and altitude, and physiological conditions, such as age and body weight, are thought to influence medicinal animal homeostasis and material quality by altering endocrine functions for primary and secondary metabolite formation. However, the currently available methods for evaluating complex components of traditional Chinese animal medicines have insufficient sensitivity and specificity.

Purpose: Characterizing the primary/secondary metabolomes of medicinal animals is essential for understanding their material basis, controlling product quality, and reflecting on distribution interactions.

Comparison of two species of Notopterygium by high-performance liquid chromatography-photodiode array detection-electrospray ionization tandem mass spectrometry.

Notopterygium incisum Ting ex H.T. Chang (N.

Differentiation of four pairs of furocoumarin isomers by electrospray ionization tandem mass spectrometry.

A mass spectrometric method based on the combined use of electrospray ionization, collision-induced dissociation (CID) and ion-trap time-of-flight (IT-ToF) tandem mass spectrometry was used to identify four pairs of furocoumarin isomers. Our results show some characteristic mass spectrometric differences in isomeric furocoumarins. In the MS(2) spectra, the relative abundance ratios of m/z 131 [M + H - 2CO](+) to m/z 143 [M + H - CO(2)](+) and m/z 115 [M + H - CO-CO(2)](+) of psoralen are less than one, but both ratios are greater than one in the case of isopsoralen.

[Studies on chemical constituents of aerial parts of Ammopiptanthus mongolicus].

Objective: To investigate the chemical constituents of the aerial parts of Ammopiptanthus mongolicus.

Method: The chemical constituents were isolated by various column chromatographic methods. The structures were identified by spectral data.

[Determination of gold in geological sample by inductively coupled plasma mass spectrometry after carbon nanofibers separation and preconcentration].

A novel method for the determination of Au by inductively coupled plasma mass spectrometry (ICP-MS) is described. Au can be separated and preconcentrated with a micro-column packed with carbon nanofibers as an adsorption material. The adsorption and elution behaviors of Au3+ on carbon nanofibers were systematically investigated under dynamic conditions.

[Slurry sampling for direct determination of trace cadmium in amber by graphite furnace atomic absorption spectrometry].

A method for the direct determination of trace cadmium in amber by graphite furnace atomic absorption spectrometry (GFAAS) with slurry sampling was described. Palladium nitrate was used as a matrix modifier to eliminate the interference. Slurry stability and the influences of the matrix modifier, ashing/atomization temperature and coexistent ions on analytical signals were investigated in detail.

[Determination of Fe, Al and Ni in ZrO2 by electrothermal vaporization ICP-AES].

A novel method for the analysis of ZrO2 powder via in-situ separation of the matrix from the analytes is described, based on the use of a polytetrafluorethylene (PTFE) emulsion as a chemical modifier. The main affecting factors, including the flow rate of carrier gas and auxiliary carrier gas, ashing temperature and time, matrix concentration, signal record time, and vaporization temperature and time, were investigated systematically. Under the optimum operating conditions, the detection limits (DL) of analytes vary from 4.

Honokiol inhibits arterial thrombosis through endothelial cell protection and stimulation of prostacyclin.

Aim: To study the effect of honokiol on arterial thrombosis and endothelial cells.

Methods: Rabbit platelet aggregation was performed with Borns turbid method. Thrombosis was produced by the endothelial injury stimulated with electric current.

[Direct determination of trace elements in human serum by electrothermal vaporization ICP-AES].

A method for direct determination of trace elements in human serum by electrothermal vaporization inductively coupled plasma atomic emission spectrometry (ETV-ICP-AES) with slurry sampling was developed. A polytetrafluoroethylene (PTFE) emulsion was used as a fluorinating agent to promote the vaporization of the analytes from graphite furnace, resulting in a remarkable improvement of analytical performances for the recommended method. The main factors affecting emission signals of the interested elements were investigated.

[Direct determination of trace arsenic in coal by slurry-sampling graphite-furnace atomic absorption spectrometry].

A method for direct determination of trace arsenic in coal by slurry-sampling Graphite-Furnace Atomic Absorption Spectrometry (GFAAS) was developed, using Pd as a matrix modifier. The effects of slurry stability, particle size of sample, matrix modifiers, ashing temperature, atomization temperature, and common coexisting components on the signal intensity of arsenic were investigated. Under optimized operating conditions, the detection limits and relative standard deviation of this method were 0.

[Pharmacokinetics of honokiol in rats].

Objective: To investigate the pharmacokinetics of honokiol in rats.

Methods: Honokiol injection was delivered by vein injection to SD-rats. The blood samples were gathered at a series of time lags.

Blockade of paeoniflorin on sodium current in mouse hippocampal CA1 neurons.

Aim: To study the blockade of paeoniflorin (Pae) on I(Na) in the acutely isolated hippocampus neurons of mice.

Methods: The whole-cell patch clamp technique was used.

Results: Pae inhibited I(Na) in frequency-dependent and concentration-dependent manners, with an IC50 of 271 micromol/L.

[Direct analysis of trace elements in tobacco using electrothermal vaporization ICP-AES].

A method for direct analysis of trace elements (Cu, Fe, Al, Cr and Mn) in tobacco by slurry sampling electrothermal vaporization (ETV) ICP-AES using polytetrafluoroethylene (PTFE) emulsion as a chemical modifier was described. The main factors affecting the analytical signals were studied. The detection limits of this method for the determination of the interesting elements were in the range of 3.

[Variation of endogeneous ET, CGRP and NO in myocardial ischemia in rats and the regulatory effect of xinshuping].

Objective: To observe the variation of ET and CGRP contents in ischemic heart, and NO level in serum of myocardial damaged rats, and their regulation when with the protection of Xinshuping, a traditional Chinese medicine compound.

Method: The models of myocardial ischemia were prepared by subcutaneous injection of isoproterenol or by ligation of coronary artery.

Result: ET content in myocardium was significantly increased (P < 0.

[Determination of cichoric acid in Echinacea purpuea].

Objective: To analyze the natural change rule of active components of E. purpuea by measuring content of cichoric acid.

Method: Reverse HPLC method was used.