[Effect of hepatocyte growth factor gene transfection on biological features of lymphoma cells].

Objective: To investigate the biological effect of hepatocyte growth factor (HGF) on HGF gene-transfected Raji cells.

Methods: Total RNA was extracted from human hepatic tissue, HGF gene cDNA was amplified by RT-PCR, and then cloned into vector pVITRO2-mcs to construct recombinant eukaryotic expression vector pVITRO2-mcs-HGF. The recombinant vector was transfected to Raji cells, and the stably transfected cells were selected by homomycin B in serial passages, and confirmed by real-time fluorescent quantitative PCR, ELISA, immunocytohistochemistry.