Gold nanoparticle-based signal enhancement of an aptasensor for ractopamine using liquid crystal based optical imaging.

The authors report on a method for the determination of ractopamine (RAC) via liquid crystal (LC) optical imaging and gold nanoparticle-induced signal enhancement. The gold nanoparticles (AuNPs) were blended with the desired concentrations of RAC, and this is found to strongly improve the performance of the assay. The RAC aptamers were immobilized on the self-assembled film of a glass slide for specific recognition of RAC.

Protein-protein interaction and SNP analysis in intraductal papillary mucinous neoplasm.

Intraductal papillary mucinous neoplasm (IPMN) is a type of tumor that grows within the pancreatic ducts. It is a progress from hyperplasia to intraductal adenoma (IPMA), to noninvasive carcinoma, and ultimately to invasive carcinoma (IPMC). The objective of this study was to explore the molecular mechanism of the progression from IPMA to IPMC.

[The effect on the pro-inflammatory role of N9 microglia exposured to hyperoxia after preconditioning with lipopolysaccharide in vitro].

Aim: To observe the time-dapendcrt expression of TLR4 and TNF-α of N9 microglia exposured to normobaric hyperoxia after preconditioning with lipopolysaccharide in vitro and to explore the role of hyperoxia on the pro-flammation response of microglia and mechanism.

Methods: N9 microglia cell line cultured in vitro was randomly divided into six groups(n=3): normoxia group, sLPS group(100 ng/mL), hLPS group(1 mg/L), hyperoxia group, hyperoxia+sLPS group(100 ng/mL), hyperoxia+hLPS group(1 mg/L). Each of the last two groups, 30 min after pretreatment with different level of LPS, was subjected to 900 mL/L hyperoxia for various times (2 h, 6 h, 12 h, 24 h and 48 h).

[Expression of cTnT in rabbit hearts during myocardial ischemia and its postmortem stability].

Objective: To study the postmortem stability of cTnT as well as its expression alteration, and to evaluate it in the diagnosis of early myocardial ischemia in forensic practice.

Methods: Animal model of early myocardial ischemia was established by rabbit coronary artery ligation. The expression of cTnT in myocardium at different postmortem intervals was detected using immunohistochemistry and analyzed using imaging technique and statistics.

[The expression of ubiquitin in the healing process of incised wounds of rat's skin and its significance to forensic medicine].

Objective: To evaluate the changes of ubiquitin expression in incised wounds of the rat skin.

Methods: The testing rat groups were subjected to incised skin wounds, with normal rat skin used as control. The expression level of ubiquitin was assessed using immunohistochemistry and imaging analysis technique on skin samples taken at 1, 3, 6, 12h, and on day 1, 3, 6, 10, and 14d after injury.

[Recent advancement in relationship between DNA degradation and postmortem interval].

Determination of postmortem interval (PMI) is one of the most valuable subjects in forensic practice. It, however, is often very difficult to accurately determine the PMI in daily practice. Forensic DNA technology has recently been used to estimate the PMI.