miR-135b-5p Suppresses Androgen Receptor-Enhanced Hepatocellular Carcinoma Cell Proliferation via Regulating the HIF-2α/c-Myc/P27 Signals in vitro.

Introduction: Hepatocellular carcinoma (HCC) accounts for more than 90% of liver cancers and is ranked as the fifth most common malignancy. Androgen receptor (AR) may promote the progression of HCC at an early stage of the disease. However, this study identified miR-135b-5p as an AR upstream regulator can suppress AR protein expression and inhibit HCC proliferation, consistent with the idea that AR expression is negatively correlated with HCC progression.

Strepchazolins A and B: Two New Alkaloids from a Marine Streptomyces chartreusis NA02069.

Two new alkaloids, strepchazolins A () and B (), together with a previously reported compound, streptazolin (), were isolated from a marine actinomycete, NA02069, collected in the Coast of Hainan Island, China. The structures of new compounds were determined by extensive NMR, mass spectroscopic and X-ray crystallographic analysis, as well as modified Mosher's method. Compound showed weak anti- activity with the MIC value of 64.

Pseudonocardia nematodicida sp. nov., isolated from mangrove sediment in Hainan, China.

Two aerobic, Gram-stain positive actinobacterial strains with nematicidal activity, designated HA11164(T) and HA12591, were isolated from mangrove sediments in Hainan, China. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strains HA11164(T) and HA12591 belong to the genus Pseudonocardia and are closely related to Pseudonocardia carboxydivorans (with the similarities of 98.30 and 98.

Nocardiopsis mangrovei sp. nov., isolated from mangrove sediment.

Two Gram-positive actinobacterial strains, designated HA11166(T) and HA12420, were isolated from mangrove sediments in Hainan, China. The bacterial cells grew with 0-9 % (w/v) NaCl, at 15-40 °C and pH 5.0-10.

Gracilibacillus marinus sp. nov., isolated from the northern South China Sea.

Two gram-positive, aerobic, spore-forming, rod-shaped bacteria, designated HB09003(T) and HB12160, were isolated from seawater and sediment in the northern South China Sea, respectively. Cells were found to be motile by means of peritrichous flagella. The strains were found to grow with 0-15 % (w/v) NaCl, at 10-45 °C and pH 5.

A new diterpenoid from Rhizophora apiculata.

A new diterpenoid, 15(S)-isopimar-7-en-1-oxo-15,16-diol (1), was isolated from the stems of mangrove plant Rhizophora apiculata. The structure of the new compound was elucidated by MS, IR, 1D, and 2D NMR techniques, including HMQC, HMBC, and NOESY correlations. In addition, seven known constituents were isolated from this plant for the first time.

[Seven kinds of new SCCmec type in Methicillin-resistant Staphyloccus aureus and their susceptibility to the antibiotics].

In order to explore the resistance and the staphylococcal chromosome cassette mec (SCCmec) types of Methicillin-resistant S. aureus (MRSA) in the area of Haikou, 686 strains of MRSA had been distinguished from 1174 strains of S. aureus using PBP2a testing.

[Two kinds of new Sccmec type in Methicillin-resistant Staphylococcus aureus and their susceptibility to the antibiotics].

In order to explore the resistance, epidemical distribution and the staphylococcal chromosome cassette mec (SCCmec) types of Methicillin-resistant S. aureus (MRSA) in the area of Haikou, the resistance and SCCmec type of MRSA isolated from clinical sample has been tested using the K-B Agar diffuse, E-test and multiplex PCR strategy, resulting that two kinds of new SCC mec types were found in them. Type-newl possess three loci of B, M, F.

[Archaeal diversity associated with sponge Pachychalina sp. analyzed by culture-independent approach].

With culture-independent approach, microbial total DNA was directly extracted from Pachychalina sp. Using the total microbial DNA as template, archaeal 16S rDNAs were amplified by PCR with universal primers. Amplified products were cloned into T-vector and secondarily amplified by PCR.