Multiregion WES of metastatic pancreatic neuroendocrine tumors revealed heterogeneity in genomic alterations, immune microenvironment and evolutionary patterns.

Pancreatic neuroendocrine tumors (PanNETs), though uncommon, have a high likelihood of spreading to other body parts. Previously, the genetic diversity and evolutionary patterns in metastatic PanNETs were not well understood. To investigate this, we performed multiregion sampling whole-exome sequencing (MRS-WES) on samples from 10 patients who had not received prior treatment for metastatic PanNETs.

Differential gene expression and potential regulatory network of fatty acid biosynthesis during fruit and leaf development in yellowhorn (), an oil-producing tree with significant deployment values.

(yellowhorn) is a woody oil plant with super stress resistance and excellent oil characteristics. The yellowhorn oil can be used as biofuel and edible oil with high nutritional and medicinal value. However, genetic studies on yellowhorn are just in the beginning, and fundamental biological questions regarding its very long-chain fatty acid (VLCFA) biosynthesis pathway remain largely unknown.

Telomere-to-telomere and haplotype-resolved genome assembly of the Chinese cork oak ().

The , a deciduous broadleaved tree species, holds significant ecological and economical value. While a chromosome-level genome for this species has been made available, it remains riddled with unanchored sequences and gaps. In this study, we present a nearly complete comprehensive telomere-to-telomere (T2T) and haplotype-resolved reference genome for .

Retinoic acid delays murine palatal shelf elevation by inhibiting Wnt5a-mediated noncanonical Wnt signaling and downstream Cdc-42/F-actin remodeling in mesenchymal cells.

Background: Mammalian palatal shelves erupted from maxillary prominences undergo vertical extention, transient elevation, and horizontal growth to fuse. Previous studies in mice reported that the retinoic acid (RA) contributed to cleft palate in high incidence by delaying the elevating procedure, but little was known about the underlying biological mechanisms.

Methods: In this study, hematoxylin-eosin and immunofluorescence staining were employed to evaluate the phenotypes and the expression of related markers in the RA-treated mice model.

Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub.

Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C.

Unique gene duplications and conserved microsynteny potentially associated with resistance to wood decay in the Lauraceae.

Wood decay resistance (WDR) is marking the value of wood utilization. Many trees of the Lauraceae have exceptional WDR, as evidenced by their use in ancient royal palace buildings in China. However, the genetics of WDR remain elusive.

Gapless genome assembly of azalea and multi-omics investigation into divergence between two species with distinct flower color.

The genus (Ericaceae), with more than 1000 species highly diverse in flower color, is providing distinct ornamental values and a model system for flower color studies. Here, we investigated the divergence between two parental species with different flower color widely used for azalea breeding. Gapless genome assembly was generated for the yellow-flowered azalea, .

Efficient synthesis of N-acetyllactosamine using immobilized β-galactosidase on a novel 3D polymer support.

A novel polymer support was prepared by curing of epoxy resin in ethanol solution in the macropores of a melamine sponge. The produced polymer gel could uniformly deposit on the surface of melamine in either porous or nonporous morphology. The composite sponge with porous coating can be used as a large-sized and well-mass transferred support for the immobilization of β-galactosidase from Bacillus circulans through method of adsorption and crosslinking, and a column reactor was made for the preparation of N-acetyllactosamine in a sealed circulation way.

[Effects of soil surface electric field on aggregates breakdown and water erosion in black soil region of Northeast China].

Through quantitatively adjust soil electric field, we investigated the effect of soil electric field on aggregate stability and soil erosion in black soil region of Northeast China with the experiments of wet sieving and rainfall simulation. Results showed that: 1) Soil surface potential absolute value and electric field strength increased with the decreases of electrolyte concentration in bulk solution. Soil electric field strength could reach to 10 V·m.

Surface properties and suspension stability of low-temperature pyrolyzed biochar nanoparticles: Effects of solution chemistry and feedstock sources.

Intensive application of biochar requires better understanding of their environmental behaviors such as stability, fate, and mobility. The release of bulk biochar into biochar nanoparticles (NPs) may bring risks because of their potential flowing into downstream water bodies with nutrients/containments attached. Low-temperature pyrolyzed biochars, namely fruit tree branch biochar of 350/450/550 °C (FB350, FB450 and FB550), corn straw biochar of 350 °C (CB350) and peanut straw biochar of 350 °C (PB350), were produced, and their NPs were extracted.

A meta-analysis of surgical decompression in the treatment of diabetic peripheral neuropathy.

Background: Over the last decade, surgical decompression procedures have been commonly used in the treatment of diabetic peripheral neuropathy (DPN). However, the effectiveness of them remains to be proved.

Methods: A comprehensive literature search of databases including PubMed-Medline, Ovid-Embase, and Cochrane Library was performed to collect the related literatures.

[Analysis of Spatio-temporal Distribution and Variation Characteristics of Aerosol Optical Depth over the Northwest of China by MODIS C6 Product].

This work compared the collection 6 MODIS aerosol optical depth products with sun photometer data from AERONET, and evaluated the applicability of C6 merged data in the northwest of China. The spatio-temporal variation characteristics and influencing factors of AOD over northwest of China were analyzed by MODIS/AQUA C6 MYD08-M3 products from 2006 to 2015, and the results showed that Southern Xinjiang and Guanzhong region of Shanxi province had relatively high AOD, whereas the south of Qinghai and Gansu province had low values; Junggar Basin was the incremental area of AOD for the first 5 years and second 5 years, whereas converse result existed in Qaidam Basin and Hetao area. Temporally, the yearly mean AOD ranged from 0.

[Effect of Biochar on Soil Greenhouse Gas Emissions in Semi-arid Region].

This study aimed to investigate the effects of biochar addition on the emission of greenhouse gases from farmland soil in semi-arid region. Through an in-situ experiments, the influence of sawdust biochar(J) and locust tree skin biochar (H) at three doses (1%, 3%, and 5% of quality percentage) on C2, CH4 and N2O emissions were studied within the six months in the south of Ningxiaprovince. The results indicated that soil CO2 emission flux was slightly increased with the addition doses for both biochars, and the averaged CO2 emission flux for sawdust and locust tree skin biochar was enhanced by 1.

[Characters of soil organic matter functional groups in the fields planted with alfalfa (Medicago sativa) for different years in hilly regions of south Ningxia, Northwest China].

Soil samples were collected from the fields planted with alfalfa for different years in the hilly region of southern Ningxia, and the technique of synchrotron radiation-fourier transform infrared spectroscopy (SR-FTIR) was adopted to study the characters of soil organic carbon (SOC) functional groups in different sizes of soil aggregates. Planting alfalfa changed the contents of SOC functional groups and their distribution in soil aggregates. With the increasing year of planting alfalfa, the SOC content, percentage of macro-aggregates (> 0.

[Responses of winter wheat photosynthetic characteristics and chlorophyll content to water-retaining agent and N fertilizer].

The effects of water-retaining agent (60 kg x hm(-2)) and nitrogen fertilizer (0, 225, and 450 kg x hm(-2)) on the leaf photosynthetic characteristics, chlorophyll content, and water utilization of winter wheat at jointing and grain-filling stages were studied under field conditions. In all treatments, the net photosynthetic rate, stomata conductance, intercellular CO2 concentration, water use efficiency, and chlorophyll content were greater at grain-filling stage than at jointing stage. Under nitrogen fertilization but without water-retaining agent application, the water use efficiency (WUE) of single leaf at jointing stage increased with increasing nitrogen fertilization rate, while the net photosynthetic rate, stomata conductance, intercellular CO2 concentration, and transpiration rate decreased after an initial increase.