Publications by authors named "Shi-long Chen"

Soil microorganisms play vital roles in regulating multiple ecosystem functions. Recent studies have revealed that the rare microbial taxa (with extremely low relative abundances, which are still largely ignored) are also crucial in maintaining the health and biodiversity of the soil and may respond differently to environmental pressure. However, little is known about the soil community structures of abundant and rare taxa and their assembly processes in different soil layers on the Qinghai-Tibet Plateau (QTP).

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A flexible manipulator is a versatile automated device with a wide range of applications, capable of performing various tasks. However, these manipulators are often vulnerable to external disturbances and face limitations in their ability to control actuators. These factors significantly impact the precision of tracking control in such systems.

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Background And Aims: Exploring how species diverge is vital for understanding the drivers of speciation. Factors such as geographical separation and ecological selection, hybridization, polyploidization and shifts in mating system are all major mechanisms of plant speciation, but their contributions to divergence are rarely well understood. Here we test these mechanisms in two plant species, Gentiana lhassica and G.

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Objective: To examine the anti-inflammatory effects and potential mechanisms of polypeptide from Moschus (PPM) in lipopolysaccharide (LPS)-induced THP-1 macrophages and BALB/c mice.

Methods: The polypeptide was extracted from Moschus and analyzed by high-performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Subsequently, LPS was used to induce inflammation in THP-1 macrophages and BALB/c mice.

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Fungi capable of producing fruit bodies are essential food and medicine resources. Despite recent advances in the study of microbial communities in mycorrhizospheres, little is known about the bacterial communities contained in fruit bodies. Using high-throughput sequencing, we investigated the bacterial communities in four species of mushrooms located on the alpine meadow and saline-alkali soil of the Qinghai-Tibet Plateau (QTP).

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Article Synopsis
  • Understanding population differentiation and speciation helps explain biodiversity, particularly in the Hengduan Mountains, which has a rich and unique plant life.
  • Researchers used advanced DNA sequencing to analyze genetic variations in the Gentianaceae family, discovering distinct genetic clusters based on geography within the mountains.
  • Findings revealed significant genetic differentiation between Southern and Northern populations, with the complex originating in central HM and dispersing during specific geological epochs, influenced by geographic barriers and climatic changes.
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Recovering phylogenetic relationships in lineages experiencing intense diversification has always been a persistent challenge in evolutionary studies, including in section sensu lato (s.l.).

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, ( L., Caprifoliaceae, Dipsacales) are widely distributed in China while mainly occurrs in northeast China. Few reports have been determined on the genus .

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Article Synopsis
  • The genus L. is a herbaceous group in the Celastraceae family, comprising about 60 species primarily found in the Pan-Himalayan region, but its classification and evolutionary relationships are still debated.
  • The study involved sequencing whole plastid genomes and nuclear ribosomal DNA of 48 species to re-evaluate the taxonomic standing of L. and investigate its phylogeny within Celastraceae.
  • Results supported the Angiosperm Phylogeny Group IV classification, confirmed L. as a monophyletic group, and suggested that its diversification likely occurred during the Eocene, although morphological traits may have evolved multiple times, indicating possible hybridization/introgression that warrants further investigation.
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Permafrost degradation may induce soil carbon (C) loss, critical for global C cycling, and be mediated by microbes. Despite larger C stored within the active layer of permafrost regions, which are more affected by warming, and the critical roles of Qinghai-Tibet Plateau in C cycling, most previous studies focused on the permafrost layer and in high-latitude areas. We demonstrate in situ that permafrost degradation alters the diversity and potentially decreases the stability of active layer microbial communities.

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Background: Understanding the genetic mechanisms of local adaptation is an important emerging topic in molecular ecology and evolutionary biology.

Results: Here, we identify the physiological changes and differential expression of genes among different weeping forsythia populations under drought stress in common garden experiments. Physiological results showed that HBWZ might have higher drought tolerance among four populations.

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Pterocarya stenoptera is a tree species that occurs along rivers and has high tolerance to waterlogging. Identification of waterlogging response genes in the aboveground part of P. stenoptera will increase understanding of tolerance mechanisms under root waterlogging conditions.

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Genetic mechanisms of species local adaptation are an emerging topic of great interest in evolutionary biology and molecular ecology. In this study, we compared the changes of physiological and phenotypic indexes and gene expression of four weeping forsythia populations under cold stress through a common garden experiment. Physiological and phenotypic results showed that there were differences in cold tolerance among populations.

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The structure and sequence of plastid genomes is highly conserved across most land plants, except for a minority of lineages that show gene loss and genome degradation. Understanding the early stages of plastome degradation may provide crucial insights into the repeatability and predictability of genomic evolutionary trends. We investigated these trends in subtribe Gentianinae of the Gentianaceae, which encompasses ca.

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The Qaidam Basin is the most extensive (120 000 km2) basin on the Qinghai-Tibet Plataea (QTP). Recent studies have shown that environmental selection and dispersal limitation influence the soil fungal community significantly in a large-scale distance. However, less is known about large-scale soil fungal community assemblages and its response to the elevation gradient in the high-elevation basin ecosystems.

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The Qinghai-Tibetan Plateau (QTP) and adjacent areas are centres of diversity for several alpine groups. Although it is known that the QTP acted as a source area for diversification of the alpine genus , the evolutionary processes underlying diversity in this genus, especially the formation of narrow endemics, are still poorly understood. Hybridization has been proposed as a driver of plant endemism in the QTP but few cases have been documented with genetic data.

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Background: The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites of the Tibetan antelope remains limited.

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Article Synopsis
  • The Qinghai-Tibetan Plateau (QTP) is home to many unique alpine plant species, yet the factors driving their diversity, particularly hybridization, are not well understood.
  • This study focused on three Gentiana species, analyzing their genetic relationships and evolutionary history through various genetic methods and climate modeling.
  • Results indicate that these species have distinct geographic clades, evidence of past refugia, expansion during climatic changes, and potential hybridization, emphasizing the impact of climate on their current diversity.
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Many studies have investigated patterns of soil microbial communities over large spatial scales. However, these studies mainly focused on a few sites. Here, we studied the near-surface (0-30 cm) soil microbial communities of 35 soil samples collected from most of the areas of the Qaidam Basin, which is the largest basin on the Qinghai-Tibet Plateau.

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Premise: (Saxifragaceae) is a widespread alpine species in the Qinghai-Tibetan Plateau and its flanking mountains. We developed a set of expressed sequence tag-simple sequence repeat (EST-SSR) markers to investigate the genetic diversity and evolutionary history of the species.

Methods And Results: We initially designed 50 EST-SSR markers based on transcriptome data of .

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Understanding the genetic structure and evolutionary history of plants contributes to their conservation and utilization and helps to predict their response to environmental changes. The wildflower and traditional Chinese and Tibetan medicinal plant var. is endemic to the Qinghai-Tibetan Plateau (QTP).

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An increasing number of phylogeographic studies have been conducted for plant species in the Qinghai-Tibetan Plateau (QTP) and its flanking mountains. However, these studies have mainly focused on the determination of glacial refugia and routes of inter-/post-glacial expansions. Rapid intraspecific diversification of plants in this region have not been thoroughly discussed.

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The varying topography and environment that resulted from paleoorogeny and climate fluctuations of the Himalaya-Hengduan Mountains (HHM) areas had a considerable impact on the evolution of biota during the Quaternary. To understand the phylogeographic pattern and historical dynamics of (Caprifoliaceae), we sequenced three chloroplast DNA fragments (, , and ) from 238 individuals representing 20 populations. Nineteen haplotypes (H1-H19) were identified based on 23 single-site mutations and eight indels.

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The chloroplast (cp) genome is useful in the study of phylogenomics, molecular dating, and molecular evolution. sect. is a predominantly alpine flowering plant that is valued for its contributions to medicine, ecology, and horticulture.

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