DNA intercalation makes possible superior-gain organic photoelectrochemical transistor detection.

DNA intercalation has increasingly been studied for various scenario implementations due to the diverse functions of DNA/intercalators. Nascent organic photoelectrochemical transistor (OPECT) biosensing taking place in organic electronics and photoelectrochemical bioanalysis represents a promising technological frontier in the arena. In this work, we first devise DNA intercalation-enabled OPECT for miRNA detection with a superior gain up to 17100.

[Chemical Forms and Spatial Distribution of Phosphorus in the Sediment of Sihe River].

Based on the study of the content, forms, and spatial distribution of phosphorus (P) in the surface and columnar sediments of the Sihe River, the relationships between the total phosphorus (TP) and various forms of P and the basic physical and chemical properties of sediment and their ecological significance were deeply discussed. The forms of P in the sediment were defined including soluble and loosely bound P (S/L-P), Al-bound P (Al-P), Fe-bound P (Fe-P), reductant soluble P (RS-P), Ca-bound P (Ca-P), and residual P (RES-P) using the method of selectively sequential extraction. The results indicated that (TP) was 421.

Preparation and Antimicrobial Activity of Antibacterial Silver-Loaded Polyphosphazene Microspheres.

Poly(cyclotriphosphazene-co-4,4'-diaminodiphenyl ether) (PPO) microspheres were prepared via a precipitation polymerization method, using hexachlorocyclotriphosphazene (HCCP) and 4,4'-diaminodiphenyl ether (ODA) as monomers. Silver-loaded PPO (PPOA) microspheres were generated by the in situ loading of silver nanoparticles onto the surface by Ag reduction. Our results showed that PPOA microspheres were successfully prepared with a relatively uniform distribution of silver nanoparticles on microsphere surfaces.

[Contamination Characteristics and Potential Ecological Risks of Typical Perfluoroalkyl Substances in the Water and Sediment of Coastal Tourism Resorts in Shandong Province].

Coastal tourism resorts have been developing rapidly in recent years, and the quality of their waterbodies directly affects human health. Perfluoroalkyl substances (PFASs) are found in every environmental medium and have garnered widespread social concern because of their potential ecological harm. This study investigated the contamination characteristics and potential ecological risks of 12 PFASs in the water and sediment of 20 coastal tourism resorts in Shandong Peninsula.

[Concentrations of Typical Perfluoroalkyl Acids and Contributions of Their Precursors in the Water of the Le'an River in China].

Transformation of perfluoroalkyl substance (PFASs) precursors and their contribution to PFASs pollution have become a subject of great research interest. The concentration of PFASs and their precursors and the transformation of precursors in the water samples collected from Le'an River and the effluents of sewage treatment plants (STPs) were investigated based on methods including hydroxyl radical (·OH) oxidation, extraction by solid phase, and ultra-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The results of the study revealed that the PFAS concentrations were in the range of 14.

[Spatial Distribution of Perfluoroalkyl Acids and Transformation of Their Precursors in River Water Samples and Effluents of Wastewater Treatment Plans in a Typical Tourism City].

Perfluoroalkyl acids (PFAAs) are commonly detected in various environmental media and can cause potential risks to the ecological environment and human health. The levels and spatial distribution of 15 types of PFAAs and 3 types of PFAA precursors in the main river water and effluents of three sewage treatment plants (STPs) of Rizhao City were systematically investigated based on methods such as ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), solid phase extraction and enrichment, and hydroxyl radical (·OH) oxidation to explore the pollution characteristics of PFAAs and the contribution of their precursors to environmental pollution in different water bodies of typical tourist cities. The results of this study reveal that 15 types of PFAAs and only perfluorooctane sulfoneamide (FOSA) among the precursors are detectable in most of the water samples.

Clinical application of a novel diagnostic scheme including pancreatic β‑cell dysfunction for traumatic multiple organ dysfunction syndrome.

A novel diagnostic scheme that includes pancreatic β‑cell dysfunction analysis for the diagnosis of traumatic multiple organ dysfunction syndrome (MODS) was investigated to assist in the early diagnosis and detection of MODS. Early intervention and treatment of MODS has been associated with a reduced mortality rate. A total of 2,876 trauma patients (including patients post‑major surgery) were admitted to the intensive care unit of the authors' hospital between December 2010 and December 2015 and enrolled in the present study.

Pharmacokinetic study and clinical evaluation of a slow-release 5-fluorouracil implant in pancreatic cancer patients.

The aim of this research was to study the pharmacokinetic characteristics of a slow-release 5-fluorouracil implant as well as to evaluate the clinical drug activity of this preparation in pancreatic cancer patients. Pharmacokinetic characteristics of the slow-release 5-fluorouracil implant were evaluated by examining the half-life time (T1/2) and apparent volume of distribution (Vd) in pancreatic cancer patients; the slow-release 5-fluorouracil implant was administered through interstitial chemotherapy (tumor interstitium implantation). In the drug activity study, 36 locally advanced unresectable pancreatic cancer patients were divided randomly into an experimental treatment group (n=18) and a standard treatment group (n=18).

Experimental study on sustained-release 5-Fluorouracil implantation in canine peritoneum and para-aortic abdominalis.

Objective: To observe local and systemic toxicity after sustained-release 5-fluorouracil (5-Fu) implantation in canine peritoneum and para-aortic abdominalis and the changes of drug concentration in the local implanted tissue with time.

Methods: 300 mg sustained-release 5-Fu was implanted into canine peritoneum and para-aorta abdominalis. Samples were taken 3, 5, 7 and 10 days after implantation for assessment of changes and systemic reactions.

[Effects of panthenol-glutamine on intestine of rats with burn injury and its dose-effect relationship].

Objective: To study the effects of the panthenol-glutamine on intestinal damage and motor function of intestine in rats with burn injury as well as its dose-effect relationship.

Methods: (1) Experiment 1. Ninety SD rats were divided into groups A-I according to the random number table, with 10 rats in each group.

[Mechanism of changes in intestinal epithelium permeability and its countermeasure after burn injury].

After a series of studies, we found that the intestinal permeability was increased, tight junction protein (zonula occluden-1) obviously decreased and redistributed, accompanied by an increase in expression of myosin light chain (MLC) phosphorylation in severely burned rats. After using inhibitor of MLC kinase (ML-9 2 mg/kg) or of Rho-associated kinase (Y-27632 2 mg/kg), above-mentioned changes could be alleviated. Therefore, to regulate the MLC phosphorylation of tight junction protein and perijunctional actin-myosin ring may be one of the key links to lessen the intestinal epithelium permeability after burn injury.

Therapeutic effectiveness of slow-release PLGA-oxaliplatin microsphere on human colorectal tumor-bearing mice.

The aim was to develop a slow-release poly-lactic-coglycolic acid (PLGA)-oxaliplatin microsphere and to assess the therapeutic effectiveness and safety of this preparation on colorectal tumor in vivo. The PLGA-oxaliplatin microsphere was prepared based on a spray-drying method, and the drug loading and in-vitro oxaliplatin release profile were carried out using high performance liquid chromatography. The inhibiting effect on tumor growth was examined using in-vivo subcutaneously inoculated colorectal tumor models of nude mice.

[Chemical forms and distribution of phosphorus in the sediment profiles of the Daliaohe River systems].

The Daliaohe river system in China has been seriously affected by long-term intensive industrial, urban and agricultural activities. The objective of this study was to determine the total phosphorus (TP) content and forms of phosphate in the sediments and investigate geochemical relationships between P forms and mineral matrix elements and P bioavailability. Four sediment profiles were taken in the Daliaohe river systems.

[Glutamine and immunonutrition for burn patients].

Nutritional therapy is an important determinant of immune function in burn patients. However, common nutritional supplement given to patients with extensive deep burn is still therapeutically inefficient to block nutrients utilization due to metabolic disorder. Immunonutrition, a new nutrition therapeutic modality, has been used in severely burned patients for regulating cell function, improving metabolic state, and enhancing immune function.

[Progress of burn research in metabolism and nutrition in China].

The achievements of burn metabolism and nutrition in China are briefly presented. Advance a new theory "Enterogenous Hypermetabolism". Develop a formula to calculate calorie needs in Chinese burn adults.

[Achievements in burn surgery over the past 50 years in China].

This paper reflects briefly the main advancements of clinical and scientific research in the field of burn surgery over the past 50 years in China. It includes emergency care of massive burns, resuscitation, anti-infection, prevention and treatment of internal organ injury, metabolic and nutritional support, repair of wound and rehabilitation, and special types of burns. The article also covers the researches in pathology, microbiology, immunology, cell biology, molecular biology, and tissue engineering pertaining to burn injury.

[Protective effects of glycyl-glutamine dipeptide supplement on the heart function in burn rats].

Objective: To investigate the protective effects of glycyl-glutamine dipeptide supplement on the function of myocardial dynamics in severely burned rats, and to explore its mechanism.

Methods: One hundred and thirty-six Wistar rats were randomly divided into five groups: i. e, control group (C, n = 8, without burns), burn group (B, n = 32), Gln group (Gln, n = 32), Gly group (Gly, n = 32) and Gly-Gln group (Gly-Gln, n = 32).

[Transfection and identification of the cloned strain that stably expressing glucagon like peptide-2 receptor in CaCO2 cell lines].

Objective: To establish Caco2 cell line with stable expression of glucagon like peptide-2 receptor( GLP-2R) , in order to establish an in vitro model for the study of protective mechanism of GLP-2 of the intestinal tract.

Methods: The GLP-2R/pcDNA3. 1 ( + ) plasmid was verified by restriction endonuclease and sequencing , and then it was transfected into Caco2 cells with lipofectamine.