Secretions released from mesenchymal stem cells improve spermatogenesis restoration of cytotoxic treatment with busulfan in azoospermia mice.

This study aimed at the efficacy of sequential treatment of bone marrow-derived mesenchymal stem cell secretion for busulfan-treated azoospermia in mice. The conditioned media (CM) was obtained from bone marrow mesenchymal stem cells (MSCs) or 293 cells. Chemically induced azoospermia mice received 200 μl MSC-CM or 293-CM twice a week intravenously for three consecutive weeks.

Mesenchymal stem cell-secreted factors delayed spermatogenesis injuries induced by busulfan involving intercellular adhesion molecule regulation.

The present study was designed to investigate the therapeutic effect of bone marrow MSC-derived factors on gonadotropic toxicity induced by busulfan in vivo. The conditioned media (CM) was obtained from MSCs in serum-free incubation for 48 hr and concentrated ~25-fold by ultrafiltration. The CM of HEK 293 cells was treated as control (293-CM).

[Investigation of Atmospheric Formaldehyde and Glyoxal Based on Differential Optical Absorption Spectroscopy].

This paper proposes a method to monitor atmospheric HCHO and CHOCHO with high temporal resolution based on differential optical absorption spectroscopy (DOAS) in Shanghai urban area. Based on the characteristic absorbing structure of HCHO and CHOCHO, different fitting intervals were chosen for spectral analysis in order to avoid the absorption of interfering gases and reduce the residuals of spectral analysis. The resulting optical thickness of the target gas is used to obtain HCHO and CHOCHO concentrations, which were averaged at (4.

[Distribution of Mahoganoid protein its mRNA in the testes and epididymides of mature male rats].

Objective: To localize the Mahoganoid protein and Mahoganoid mRNA in the testes and epididymides of mature male rats.

Methods: Testes and epididymides obtained from mature male SD rats (n = 20) were fixed by 4% poly formaldehyde and sliced for immunohistochemical (IHC) test and in situ hybridization (ISH) test, respectively, for detecting Mahoganoid protein and Mahoganoid mRNA.

Results: In both the 2 tests, clear brown staining was observed in Leydig cells, spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and peritubular myoid cells.

[Distribution of attractin protein and mRNA in the testis of rat of different ages].

Objective: Given the fact that the Attractin protein express widely in the testis of mature male rat, this paper aims at studying the distribution of the Attractin protein and Attractin mRNA in the testicular tissue of rats of different ages.

Methods: Testes and epididymides were obtained from newborn (8 hours after birth), prepubertal (5 days), pubertal (20 days), postpubertal (50 days) and mature (70 days) Wistar rats; the tissues were fixed and the Attractin protein and mRNA detected respectively by immunohistochemical and in situ hybridization techniques.

Results: The Attractin protein and mRNA expressed respectively within Leydig cells, primitive spermatogonia, primary spermatocytes, spermatids, Sertoli cells and peritubular myoid cells.