Publications by authors named "Shi-jiang Chen"

Objective: The integumentary and digestive system of Hirudo nipponica were studied,in order to protect the precious Chinese herbal medicine.

Methods: The histological study of integumentary and digestive system of Hirudo nipponica by histological methods were carried out by microscopical method.

Results: The body wall of Hirudo nipponica could be divided into five layers, including cuticular layer, epithelial layer, dermis layer, muscle layer and grape-like tissue.

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Objective: To investigate the reproductive performance of Hirudo nipponica under artificial cultivation, in order to provide reference for the commercial rearing and breeding industry of this species.

Methods: The mean number of cocoons, cocoon weight, hatching numbers of cocoons, and the effect of parent leeches weight and density on reproductive performance were investigated.

Results: The mean number of cocoons produced by parent leeches was 0.

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Background: Antrodia camphorata, a traditional Chinese medicine, is widely used in the treatment of liver diseases and cancers. Anti-inflammatory properties have also been described. HSV infection represents one of the most serious public health concerns globally because of its devastating impact.

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To clear the effect of the wound to the growth of the larva of the host to the Ophiocordyceps sinensis, the wounds of same severity at the same position were made artificially to the larva and which were artificial fed at the same environment and condition. The results indicated that, over the winter, the survival rate of the wounded of the infection larva was lower than that of the healthy larva, but the weight had no significant difference between the wounded and the healthy larva. The survival rate of the wounded of the no infection larva was lower than that of the healthy larva, but except with black skin, the wounded larva with offwhite and dusty red had no influence on the variety of the weight.

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To address the role of Pr1 gene in the process of Hirsutella sinensis infecting Hepialus gonggaensis, differential expression of subtilisin-like protease gene was detected. In the present study, Pr1 gene analogues from H. sinensis were obtained by PCR strategy using specific primers designed from conserved regions of Pr1 gene reported in the GenBank.

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Unlabelled: To study the effects of the integrated pest control techniques on growth of host larvae of Cordyceps sinensis.

Method: The integrated pest control techniques were compared with conventional techniques to evaluate the effects on growth of host larvae.

Result And Conclusion: The results showed that the techniques had broken the balance of the microbial living in the material, produced effective inhibition on the pests, raised the survival rate and promoted the growth of the host larvae at the same time.

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Objective: To study the effects of four kind of slow-release fertilizers on yield and quality of Coptis chinensis.

Method: One to three years C. chinensis was fertilized with slow-release fertilizers twice in April and in September.

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Objective: To study the changes of soluble saccharides and amino acids during the growing period of Coptis chinensis.

Method: Amino acid contents were estimated by Indene tri-ketone colorimetric method; saccharide contents were determined by anthracene ketone method.

Result And Conclusion: The contents of soluble saccharides and amino acids in different organs were low in plants of 1-2 years old.

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Objective: To study the changes of nutrient contents in Chinese goldthread soil before and after planting Coptis chinensis.

Method: The soil in Shizhu, Chongqing, was analyzed for nutrient contents.

Result And Conclusion: The results indicated that the preplanted soil was strongly acidic and the levels of organic matter, total nitrogen and potassium were high.

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Objective: To study the dynamic change of the ingredients of Coptis chinensis and evaluate the quality of the crude drugs from main producing areas.

Method: The ingredients of samples from Shizhu and Wuxi counties in Chongqing, Hongya and Dayi counties in Sichuan, Zhenping county in Shanxi, Lichuan county in Hubei were analyzed for berberine, coptisine, jatrorrhizine, palmatine and the total alkaloids by HPLC and UV methods.

Result And Conclusion: The contents of measured indexes were mostly highest in 5-years-old C.

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Objective: To supply the basis of the breeding the host of parasite of Cordyceps sinensis for it's natural culturing, by studying the biology of the host of parasite of C. sinensis, Hepialus biruensis.

Method: The host of parasite was collected in field, eclosion, mating and spawning in the different conditions were made, and it's biology and the regularity of the growth and development were observed.

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