Pile-up effect in near-infrared single-pixel imaging with an incoherent light source.

Single-pixel imaging (SPI), which offers high-throughput measurement capabilities and a simple structure, has promising applications in near-infrared single-photon imaging. Nevertheless, the low saturation count rate of near-infrared single-photon detectors often leads to photon pile-up effects. This paper delves into the influence of these effects on passive SPI under both random matrix modulation and Hadamard matrix modulation and offers corresponding noise removal solutions.

Treatment of Preschool Children With Autism Spectrum Disorder: A Trial to Evaluate a Learning Style Profile Intervention Program in China.

Objective: To investigate whether the provision of learning style profile (LSP) training improves development in preschool children with autism spectrum disorder (ASD) in China and to describe the characteristics of children who benefit from the intervention.

Methods: Eighty-one children aged 36 to 72 months who were diagnosed with ASD for the first time were recruited for the intervention group. All of them received 24 weeks of LSP training, consisting of hospital- and home-based training.

[Relationship of ADC histogram parameters with pathological grade and lymph node metastasis of prostate cancer].

Objective: To investigate the relationship between the apparent diffusion coefficient (ADC) histogram parameters based on the whole tumor and the pathological grade and lymph node metastasis (LNM) of PCa.

Methods: This retrospective study included 82 cases of PCa confirmed pathologically and subjected to MRI preoperatively. We obtained a series of ADC histogram parameters, such as ADCmean, ADCmedian, ADC25%, ADC75%, entropy, and histogram width, by processing the ADC images via the Firevoxel Post-Processing and the SPSS24 software.

Protein structure determination using a Riemannian approach.

Protein NMR structure determination is one of the most extensively studied problems. Here, we adopt a novel method based on a matrix completion technique - the Riemannian approach - to rebuild the protein structure from the nuclear Overhauser effect distance restraints and the dihedral angle restraints. In comparison with the cyana method, the results generated via the Riemannian approach are more similar to the standard X-ray crystallographic structures as a result of the simple but powerful internal calculation processing function.

An Extreme Learning Machine Based on Artificial Immune System.

Extreme learning machine algorithm proposed in recent years has been widely used in many fields due to its fast training speed and good generalization performance. Unlike the traditional neural network, the ELM algorithm greatly improves the training speed by randomly generating the relevant parameters of the input layer and the hidden layer. However, due to the randomly generated parameters, some generated "bad" parameters may be introduced to bring negative effect on the final generalization ability.

Improvement of the Immunogenicity of Porcine Circovirus Type 2 DNA Vaccine by Recombinant ORF2 Gene and CpG Motifs.

Nowadays, adjuvant is still important for boosting immunity and improving resistance in animals. In order to boost the immunity of porcine circovirus type 2 (PCV2) DNA vaccine, CpG motifs were inserted. In this study, the dose-effect was studied, and the immunity of PCV2 DNA vaccines by recombinant open reading frame 2 (ORF2) gene and CpG motifs was evaluated.

[Effect of the complex of casein phosphopeptide and amorphous calcium phosphate on reducing enamel demineralization and promoting remineralization].

Objective: To investigate the effect of the complex of casein phosphopeptide and amorphous calcium phosphate (CPP-ACP) on the demineralization and remineralization of dental enamel in vitro.

Methods: Premolars extracted from patients receiving orthodontic treatment were cut into two slabs, which were embedded and polished. The slabs were randomly divided into non-acid etching group, acid etching group (immersed in 37.

[Evaluation of four kits for screening HIV antibody].

Objective: To Evaluate four kits for screening HIV antibody by comparing and analyzing the HIV antibody screening positive results and Western Blot (WB) test results.

Methods: From January 2004 to June 2009, three ELISA kits (Zhongshan, Biomérieux and Livzon) were used for initial screening HIV antibody. The reactive positive samples were reexamed by initial ELISA kit and a rapid kit (Abbot Determine HIV-1/2).

The antibody against a nuclear autoantigenic sperm protein can result in reproductive failure.

To study whether the antibody against the testis form of the nuclear autoantigenic sperm protein (tNASP) could result in reproductive failure, we successfully cloned and expressed a 339-bp cDNA fragment of mouse tNASP (mtNASP). Using mouse as a model, recombinant mtNASP (rmtNASP) and a synthetic peptide, human tNASP(393-408) (htNASP(393-408)), were investigated for their antifertility effect. Active immunization with rmtNASP or the synthesized peptide raised high antibody titers in the immunized mice.

[Correlations between preS1-antigen, HBV-DNA and HBV serum markers in patients with chronic hepatitis B].

Objective: To study the correlations between preS1 antigen, HBV-DNA and hepatitis B virus (HBV) serum markers in patients with chronic hepatitis B.

Methods: The HBV markers, preS1 antigen and HBV-DNA were determined using enzyme- linked immunosorbent assay and quantitative PCR in 1158 patients with chronic hepatitis B.

Results: In these patients, the HBV-DNA positivity rate was 68.

Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by anti-human sperm membrane protein 1 antibody.

Aim: To investigate the possible functions of human sperm membrane protein (hSMP-1) in the process of fertilization.

Methods: A 576-bp cDNA fragment of HSD-1 gene coding for the extracellular domain of hSMP-1 was cloned and expressed. The localization of this protein on human and mouse sperm was determined by indirect immunofluorescent staining by using anti-recombinant hSMP-1 (anti-rhSMP-1) antibodies.

[Cloning, expression and purification of human nuclear autoantigenic sperm protein (hNASP) and preparation of its polyclonal antibody].

Objective: To acquire the purified human nuclear autoantigenic sperm protein (hNASP) and its polyclonal antibody for investigating the possible functions of hNASP involved in fertilization.

Methods: The coding sequence of hNASP gene was amplified from human testis RNA with specific primers, and the PCR product was cloned first into pMD-18T and then into pET-28a ( + ) after restriction digestion with BamH I and Hind III. The fusion protein was expressed in E.

[Establishment and application of the approach to detecting two biovars of Ureaplasma urealyticum in human semen].

Objective: To establish the approach to detecting two biovars of Ureaplasma urealyticum (Uu) in human semen and to investigate the relationship between the two biovars of Uu infection and the quality of human semen.

Methods: Based on the 16S-23S rRNA intergenic spacer region, three pairs of primers were designed, the species specific primer and two biovars primers (Parvo primer and T960 primer). The two biovars of Uu were detected in the semen from 949 men by semen culture and PCR assay.