Determination and tissue distribution comparisons of gentiopicroside after oral administration of raw and wine-Processed Gentiana Radix.

Gentiana Radix is one of the most often used drugs in traditional Chinese medicine. Stir frying with yellow wine is the most common processing method. To clarify the principle of processing, an experiment was carried out to compare the tissue distribution of the typical constituent after oral administration of raw G.

Rapid Characterization and Identification of Chemical Constituents in before and after Wine-Processed by UHPLC-LTQ-Orbitrap MS.

is used in traditional Chinese medicine and has functions of clearing heat and drying dampness, as well as purging liver and gallbladder fire. A highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed by using ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap) in crude and wine-processed . Based on the accurate mass measurement (<5 ppm), retention times, and MS fragmentation ions, 52 constituents were unambiguously or tentatively characterized from , including 21 iridoids, 11 flavonoids, 19 xanthones, and a triterpenoid.