Aim: To identify the structure of the acid-catalyzed product of strictosamide and explore the reaction mechanism.
Methods: The acid-catalyzed reaction process of strictosamide was monitored by HPLC, and a macroporous resin was used to purify the reaction solution. The structure of the product was confirmed by MS, NMR, and ROESY spectra.
Objective: To determine the content of polyphenol contained samples, in order to provide basis for comprehensive quality evaluation on Echinacea extracts.
Method: LC-MS was used for qualitative analysis on relevant compounds of the samples of Echinacea extracts. Optimized USP methods, RP-HPLC and QAMS (quantitative analysis of multi-components by single-marker) were used to determine the content of polyphenol.