Varisized 3D-Printed Lunate for Kienböck's Disease in Different Stages: Preliminary Results.

Objective: To evaluate the feasibility of arthroplasty with varisized three-dimensional(3D) printing lunate prosthesis for the treatment of advanced Kienböck's disease (KD).

Methods: From 2016 November to 2018 September, a retrospective study was performed for the patients of KD in our hospital. Five patients (two males, three females) were included in this study.

[Down-regulation of HDAC6 Expression Promotes Apoptosis of Human Leukemia K562 Cells].

Objective: To study the promoting-apoptosis effect of HDAC6 on the human leukemia cells and its mechanism.

Methods: The siRNA interference technology was used to inhibit the expression of HDAC6 gene, the RT-PCR and Western blot were used to detect the expression of HDAC6 and related signal pathway proteins respectively, the flow cytometry and Hoechest staining were used to detect the apoptosis and morphology changes of K562 cells.

Results: Compared with the periphal blood monocyte and bone marrow stromal cells of healthy volunteers, the expression level of HDAC6 in leukemia cell lines was up-regulated significantly(P<0.