Protective effects of losartan in mice with chronic viral myocarditis induced by coxsackievirus B3.

Aim: To investigate whether losartan has protective effects in mice with chronic viral myocarditis induced by coxsackievirus B3 (CVB3).

Main Methods: Thirty two male Balb/c mice were intraperitoneally injected with CVB3 (10×TCID50) to induce chronic viral myocarditis (CVM). Losartan at 12.

[Study on the stability of the anergic CD8(+);NKT cells in vitro].

Aim: To study the CD8(+);NKT cell stability function.

Methods: Splenic lymphocytes from C57BL/J mice were treated with Staphylococcal enterotoxin B (SEB) and then cultured in vitro. Day 10, 20, 30 and freezen effector cells were harvested and used.

[Identification of cell division of CD8+ NKT cells in vitro].

Aim: To measure the in vitro cell division ability of CD8(+);NKT cells by CFSE staining and flow cytometry(FCM).

Methods: Fresh spleen lymphocytes of C57BL/J mice were separated and stained with CFSE, and then stimulated by ConA and LPS for 3 d, and by SEB for 5 d and 10 d respectively. The stimulated cells were harvested and analyzed for CD69 expression on the cell surface and the ability of cell division using FCM.