Success rates of fertilization versus intracytoplasmic sperm injection in men with serum anti-sperm antibodies: a consecutive cohort study.

Antisperm antibodies (ASAs) are assumed to be a possible causative factor for male infertility, with ASAs detected in 5%-15% of infertile men but in only 1%-2% of fertile ones. It remains unclear whether ASAs have an adverse effect on the outcome of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). This study investigated differences in the rates of fertilization, pregnancy, and live births associated with serum ASA-positive and ASA-negative men following IVF or ICSI.

Hematopoietic stem and progenitor cells can be enriched by implanting biomaterial into spatium intermusculare.

Hematopoietic stem and progenitor cells (HSPCs) have been used successfully to treat patients with cancer and disorders of the blood and immune systems. In this study, we tried to enrich HSPCs by implanting biomaterials into the spatium intermusculare of mice hind limbs. Gelatine sponges were implanted into the spatium intermusculare of mice and then retrieved after 12 days.

[Effects of rhBMP-7 on osteoblast differentiation of murine bone marrow mesenchymal stem cells in vitro].

Aim: To study the effects of recombinant human bone morphogenetic protein-7 (rhBMP-7) on osteoblast differentiation of murine bone marrow mesenchymal stem cells (BMSCs).

Methods: The BMSCs were collected from murine bone marrow by density gradient centrifugation. Cells were adherent cultured and expanded in vitro.

[Association of susceptibility to chronic rhinosinusitis with genetic polymorphisms of IL-4 and IL-10].

Objective: To investigate the relationship between the promoter polymorphism of IL-4 and IL-6 and chronic rhinosinusitis (CRS).

Methods: One hundred and twenty-three patients with CRS and 239 healthy controls in Shanghai region were chosen in this study. The genotype of IL-4 gene -33T>C and -590C>T were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and the genotype of IL-10 gene -1082A>G was determined using amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) method.

[Suppression of Aurora-A by RNA interference inhibits laryngeal cancer Hep-2 cell growth].

Objective: To investigate the effects of knockdown of Aurora-A by RNA interference on laryngeal cancer Hep-2 cell growth in vitro and in vivo.

Methods: A plasmid containing siRNA against Aurora-A was constructed and transfected into human laryngeal cancer cell line Hep-2. Measurements included the CCK-8 assay for viability and proliferation, Transwell assay for invasion, colony formation assay for cell anchorage-independent growth.

High-throughput analysis of DNA fragments using a miniaturized CE system combined with a slotted-vial array sample introduction system.

An automated nanoliter sample introduction system was combined to a liquid-core waveguide (LCW)-based microfluidic CE system for high-throughput analysis of DNA fragments. The main component of the sample introduction system was a motor-driven plate, on which a circular array of bottom-slotted vials containing sample/buffer solutions was placed. A 7 cm-long LCW capillary served as both the sample probe and separation channel.

Enhancement of signal-to-noise level by synchronized dual wavelength modulation for light emitting diode fluorimetry in a liquid-core-waveguide microfluidic capillary electrophoresis system.

The signal-to-noise level of light emitting diode (LED) fluorimetry using a liquid-core-waveguide (LCW)-based microfluidic capillary electrophoresis system was significantly enhanced using a synchronized dual wavelength modulation (SDWM) approach. A blue LED was used as excitation source and a red LED as reference source for background-noise compensation in a microfluidic capillary electrophoresis (CE) system. A Teflon AF-coated silica capillary served as both the separation channel and LCW for light transfer, and blue and red LEDs were used as excitation and reference sources, respectively, both radially illuminating the detection point of the separation channel.

[Experimental study of baculovirus-mediated transfection of spiral ganglion cells in rats].

Objective: To study the feasibility and the characteristics of recombinant baculovirus as spiral ganglion cells (SGC) gene transfer vector.

Methods: After the generation of baculovirus- green fluorescent protein( Bac-GFP) according to Bac-to-Bac baculovirus expression system, SGC were infected by Bac-GFP with different multiplicities of infection (MOI) and different concentrations of sodium butyrate. The transfection cell rate and mean fluorescence strength (MFS) were detected by fluorescence microscopy and flow cytometry.

[Regional evapotranspiration of different land covers based on remote sensing].

In this paper, surface energy balance system (SEBS) was extended into a regional daily evapotranspiration (ET) estimation model based on remote sensing data, and the extended SEBS was applied to estimate the regional daily ET of Huanghe-Huaihe-Haihe rivers region in Northern China Plain by using MODIS/TERRA data. An analysis was made on the estimated daily ET characteristics of different land covers in the study area by using the spatial analysis module of ArcGIS. Since there were no field observations of ET on each land cover, the estimated daily ET of different land covers was compared with each other, taking the data on April 17, 2001 as an example.

Study of recombinant human osteogenic protein-1 expressed in prokaryocyte on the repair of extracted socket in rabbits.

The study investigated the effect of recombinant human osteogenic protein-1 (rhOP-1) expressed in prokaryocyte, to promote the healing of alveolar socket. A model of rabbit extracted socket into which the composites of rhOP-1 and gelatin sponge was immediately implanted was created and the osteoinduction of rhOP-1 was assessed by histological method, quantitative measurement of calcium content and alkaline phosphatase (ALP) activity. The result of histology showed that bone healing in rhOP-1 side is 4-6 weeks earlier than that of the control side.

A simple microfluidic system for efficient capillary electrophoretic separation and sensitive fluorimetric detection of DNA fragments using light-emitting diode and liquid-core waveguide techniques.

A miniaturized CE system has been developed for fast DNA separations with sensitive fluorimetric detection using a rectangle type light-emitting diode (LED). High sensitivity was achieved by combining liquid-core waveguide (LCW) and lock-in amplification techniques. A Teflon AF-coated silica capillary on a compact 6x3 cm baseplate served as both the separation channel for CE separation and as an LCW for light transmission of fluorescence emission to the detector.

[Determination of sulfur dioxide in air by sequential injection spectrophotometry].

A sequential injection spectophotometric method for the determination of sulfur dioxide by decolorizing malachite green in an alkali medium is studied; the decreasing in color is proportional to the amount of sulfur dioxide. The loading sequence, the concentration of reagents, the amount of sample and the flow of reagent rate are optimized by univariate method. With sequential injection sample introducing, the volumes of sample and reagent are reduced greatly and the speed of determination is increased apparently, a throughput of 60 samples/h can be achieved.

[Studies on sequential injection spectrophotometric system using a reflective flow cell for process analysis].

A reflective flow cell was developed and coupled to a sequential injection system and optical fiber photometric detection system based on emitting diode light source. A multistrand bifurcated optical fiber was coupled with incident light, detector and flow cell. Optical fibers were used to carry light from the electronics unit to a reflective flow-through cell and back.

Feature characterization of microfabricated microfluidic chips by PDMS replication and CCD imaging.

This paper presents a new approach for the metrological characterization of microfabricated features on microfluidic chips, based on a combination of poly(dimethylsiloxane) (PDMS) replication and charge-coupled device (CCD) imaging. A PDMS replicate was cast from the original chip sample, and a 2-mm thick sample slice was cut from the replica at the cross-section to be studied. The digital image of the revealed structural profile was captured by a CCD camera under a microscope, and the image was processed using specially-developed algorithms for CCD image calibration and edge detection.