Publications by authors named "Shi-Kang Zhu"
A TaqMan-based real-time PCR assay for the detection of Ungulate bocaparvovirus 2.
J Virol Methods
November 2018
Article Synopsis
- Ungulate bocaparvoviruses (UBoV) 2-5 are newly identified viruses that could significantly impact swine health in China.
- A TaqMan-based real-time PCR (qPCR) test was developed to detect UBoV2, proving to be more sensitive than conventional PCR, with a lower detection limit of 9.97 copies/μL.
- This qPCR successfully identified UBoV2 in 18.1% of tested pig samples, particularly in loose stools, and demonstrated high specificity with no cross-reactivity to other viruses.
Article Synopsis
- The study sequenced the whole genomes of three strains of porcine bocaparvovirus (PBoV) using polymerase chain reaction, identifying them as part of group 3 (G3).
- Phylogenetic analysis indicated that these strains are more closely related to bocaparvoviruses from other animals rather than to other porcine strains.
- The researchers also found conserved sequences associated with phospholipase A2 activity and identified a natural recombination breakpoint in the NS1 region of PBoV G3, aiding in the understanding of PBoV epidemiology.