Epididymis cell atlas in a patient with a sex development disorder and a novel gene mutation.

This study aims to characterize the cell atlas of the epididymis derived from a 46,XY disorders of sex development (DSD) patient with a novel heterozygous mutation of the nuclear receptor subfamily 5 group A member 1 (NR5A1) gene. Next-generation sequencing found a heterozygous c.124C>G mutation in NR5A1 that resulted in a p.

[Pollution Characteristics and Health Risk of Heavy Metals in Fugitive Dust Around Zhaotong City].

In order to explore the pollution characteristics and health risks of heavy metals in fugitive dust around the urban areas of Zhaotong City, road dust and soil dust samples were collected in the Zhaoyang District of Zhaotong City in May 2019. The dust samples were suspended using a particle resuspension system to obtain PM (particulate matter with an aerodynamic diameter less than or equal to 2.5 μm).

Simulated aeromedical evacuation exacerbates burn induced lung injury: targeting mitochondrial DNA for reversal.

Background: Aeromedical evacuation of patients with burn trauma is an important transport method in times of peace and war, during which patients are exposed to prolonged periods of hypobaric hypoxia; however, the effects of such exposure on burn injuries, particularly on burn-induced lung injuries, are largely unexplored. This study aimed to determine the effects of hypobaric hypoxia on burn-induced lung injuries and to investigate the underlying mechanism using a rat burn model.

Methods: A total of 40 male Wistar rats were randomly divided into four groups (10 in each group): sham burn (SB) group, burn in normoxia condition (BN) group, burn in hypoxia condition (BH) group, and burn in hypoxia condition with treatment intervention (BHD) group.

[Pollution Characteristics and Health Risk Assessment of Heavy Metals in Road Dust from Non-ferrous Smelting Parks].

In order to explore the pollution characteristics and health risks of heavy metals in dust from roads around non-ferrous smelting activities in different regions, dust samples from urban roads, non-ferrous smelting industry park roads, and tunnel roads were collected from the Mengzi area of Yunnan Province. The dust samples were suspended on Teflon filters by re-suspension equipment to obtain PM and PM samples. Eight heavy metals (Cr, Mn, Ni, Cu, Zn, As, Cd, and Pb) were analyzed by ICP-MS.

[Physico-chemical Characteristic Analysis of PM in the Highway Tunnel in the Plateau City of Kunming].

This research aimed to determine the characteristics of the motor vehicle exhaust emissions in the plateau area. The PM was evaluated in the Caohai Tunnel of Kunming for this study by measuring the water-soluble inorganic ions, carbonaceous species, polycyclic aromatic hydrocarbons (PAHs), and inorganic elements in air samples. The results showed that the mass concentration of PM in the tunnel was about 225.

Molecular cloning, sequence analysis, and cadmium stress-rated expression changes of BTG1 in freshwater pearl mussel (Hyriopsis schlegelii).

The B cells translocation gene 1 (BTG1) is a member of the BTG/TOB family of anti-proliferative genes, which have recently emerged as important regulators of cell growth and differentiation among verteates. Here, for the first time we cloned the full-length cDNA sequence of Hyriopsis schlegelii (Hs-BTG1), an economically important freshwater shellfish and potential indicator of environmental heavy metal pollution, for the first time. Using rapid amplification of cDNA ends (RACE) together with splicing the EST sequence from a haemocyte cDNA liary, we found that Hs-BTG1 contains a 525 bp open reading frame (ORF) encoding a 174 amino-acid polypeptide, a 306 bp 5' untranslated region (5' UTR), and a 571 bp 3' UTR with a Poly(A) tail as well as a transcription termination signal (AATAAA).

[Effects of fluid resuscitation programs on the levels of inflammatory mediators during burn shock stage].

Objective: To explore the effects of different methods of fluid resuscitation on the levels of inflammatory mediators during burn shock stage.

Methods: Twenty-four miniature swine were numbered from 1 to 24 and randomly divided by EXCEL 2007 into 4 groups of succinylated gelatin, hydroxyethyl starch, Parkland and allogeneic plasma (n = 6 each). Severe burn shock model was established.

[Effects of resuscitation with different kinds of colloids on pulmonary edema in swine in shock stage of severe burn injury].

Objective: To observe and compare the effects of natural colloid and artificial colloid on pulmonary edema of swine during shock stage of severe burn injury.

Methods: Twelve Guangxi Bama miniature swine were inflicted with 40% TBSA full-thickness burn on the back, and then they were divided into natural colloid group (N) and artificial colloid group (A) according to the random number table, with six swine in each group. At post injury hour (PIH) 2, fluid resuscitation was begun.

Effect of artificial colloids on blood coagulation during shock stage of severe burn injury.

Background: There are controversies about the use of artificial colloids. This research was aimed to determine the effect of various artificial colloids on blood coagulation in the shock stage of severe burn injury.

Methods: Totally, 18 female Ba-Ma mini-pigs were subjected to a 40% total body surface third-degree flame burn under anesthesia.

[Shock modeling of large area third-degree burn in miniature swine].

Objective: To report a novel method of establishing a stable shock model of swine.

Methods: A total of 12 Guangxi BA-MA female miniature swines (aged 4-6 months) into A and B groups according to a random number table. Total body surface area (TBSA) was calculated according to the Meeb-Rubner formula.

[Combination of compound Xuanju Capsule and sildenafil for erectile dysfunction that progressively fails to respond to sildenafil].

Objective: To explore the clinical effects of Compound Xuanju Capsule (CXC) combined with sildenafil on erectile dysfunction (ED) that progressively fails to respond to sildenafil.

Methods: Totally, 36 ED patients who progressively failed to respond to sildenafil were randomly divided into a trial and a control group of equal number, the former treated with oral CXC at 3 g tid plus sildenafil at 50 mg an hour before sexual activity, while the latter with sildenafil alone at 100 mg an hour before sexual activity. After 2 months of treatment, we compared the patients' scores on IIEF-5 and their partners' scores on Treatment Satisfaction Scale (TSS) between the two groups.

[Comparison among several foam dressings in the properties of water-absorption, water-locking and air permeability].

Objective: To compare the properties of water-absorption, water-locking, and air permeability among several foam dressings, and to provide references for clinician in choosing dressings for different types of wounds.

Methods: The comparison was made among Allevyn foam dressing, Mepilex foam dressing, and Biatain foam dressing that were commonly used in clinic. NaCl and CaCl2·H2O respectively in the weight of 8.

[Analysis and prevention of postoperative complications of tubularized incised plate urethroplasty for hypospadia].

Objective: To explore the reasons and preventions of complications of tubularized incised plate urethroplasty technique for hypospadia repair.

Methods: In the study, 54 patients of 247 hypospadia patients were analyzed who had complications, using SPSS 13.0 statistics to compare their complications with anatomy reasons including positions of the meatus, development of urethral mucous membrane and glans penis and degrees of ventral curvature.

[Tangential excision of deep partial thickness burn wound during an early stage without tourniquet].

Objective: To explore the clinical efficacy of tangential excision of deep partial thickness burn wound without tourniquet during an early stage.

Methods: A total of 32 patients with a large area of deep partial thickness burn wounds were operated within 24 h post-burn. The lamellar tangential excision was on the basis of stable index of resuscitation from shock without tourniquet.

[Characterization of atmospheric volatile organic compounds in Shenyang, China].

From April, 2008 to July, 2009, 187 atmospheric samples in four different seasons were collected from five sites in Shenyang. The 108 species of VOCs were measured by using the method of pre-concentration-GC-MS. The objectives of this study were to investigate the VOCs pollution level as well as its spatial and temporal distribution, and to identify the main source in Shenyang city.

[Evaluation of the clinical curative effect of applying vacuum sealing drainage therapy in treating deep partial-thickness burn wound at the initial stage].

Objective: To evaluate the clinical curative effect of applying vacuum sealing drainage (VSD) therapy in treating deep partial-thickness burn wound at the initial stage prospectively, and to provide the basis for its clinical application.

Methods: Twenty-two patients with about 10% TBSA burn of the lower limbs, and in which partial-thickness wound exceeded 1% TBSA in each limb, were admitted to our hospital within 3 hours after burn from May 2009 to March 2010. Wounds in each patient were divided into VSD treatment group (treated with VSD therapy) and control group (treated with 10 g/L silver sulfadiazine cream) based on the principles of symmetry of location, identical deepness, and similarity in size etc.

[The relationship between rhGH and blood sugar on different ages of severe degree burned patients].

Objective: Probe the effects of rhGH on severe degree burned patients' blood sugar in different age of years.

Methods: Elected 210 patients hospitalized in the Third Affiliated Hospital of Wenzhou Medical College from January 2005 to December 2008, who were burned in 48 h, older than 18 years, ever had no diabetes and tumor history and placidly pull through shock stage. Among the patients there were 132 males and 78 females.

[Effect of different dressing materials in various combinations on wound microenvironment of donor site in burn patients].

Objective: To investigate the effect of dressing materials in various combinations on burn wound microenvironment and healing condition.

Methods: Two hundred donor sites with wounds of 0.3 mm in depth in 186 burn patients, who needed skin grafting and admitted to our ward were enrolled in study, and they were divided into A (with dressing composed of alginate + cotton pad for donor area), B (with dressing composed of vaseline gauze + cotton pad for donor area), C (with dressing composed of alginate + foam dressing for donor area), D (with dressing composed of vaseline gauze + foam dressing for donor area) groups according to random table method.

1(2,3),2(3,2),3(2,3),4(3,2)-Tetra-kis(1-benzothio-phena)cyclo-tetra-phane benzene sesquisolvate.

In the title compound, C(32)H(16)S(4)·1.5C(6)H(6), the substituted cyclo-octa-tetra-ene (COT) ring compound has approximate local D(2) point symmetry of the so-called 'saddle' form: the dihedral angles between neighboring benzo[b]thio-phene units are 61.33 (4), 61.