[Effects of MSP on Cell Cycle and EMT of Non-Small Cell Lung Cancer PC14].

Objective: To study the effect of macrophage stimulating protein (MSP) on the cell cycle of non-small cell lung cancer PC14 cells without expression of recepteur d'originenanta (RON) and MSP,and analyse its effect on PC14's epithelial mesenchymal transition (EMT) capacity.

Methods: Vitro culture PC14 (blank control),PC14-Mst1-pEGFP-N1 (stablely expressed MSP) and PC14-pEGFP-N1. Cell cycles were detected by flow cytometry and the gaps between cells during growth were measured by transmission electron microscope (TEM); RT-PCR and Western blot were used to figure out the shifts of EMT related gene expression in PC14-Mst1-pEGFP-N1 cells.

[The Correlation Between MicroRNAs in Serum and the Extent of Liver Injury].

Objectives: To investigate the correlation between the absolute quantification of the microRNAs (miR-122, miR-451, miR-92a, miR-192) in serum during acute liver injury and the extent of liver injury on rat models of CCl induced acute liver injury and mice models of acetaminophen (APAP) induced acute liver injury. Furthermore, to investigate the correlation between the absolute quantification of microRNAs in serum and the drug induced liver injury pathological scoring system (DILI-PSS).

Methods: The acute liver injury model in rat by CCl (1.

[Effects of Msp on the Proliferation, Migration and Invasion of Human Non-small Cell Lung Cancer Cells].

Objectives: To determine the effects of macrophage stimulating protein (Msp) on the proliferation, migration and invasion of human non-small cell lung cancer cells PC14.

Methods: The eukaryotic expression vector for was constructed and transfected into Msp(-)and RON(-)human non-small cell lung cancer cells PC14. The expression of mRNA in PC14 cells was observed by RT-PCR.