Objective: To investigate the role of the pannexin-1 (Panx1) protein in the invasion and migration of testicular cancer Tcam-2 cells and its possible action mechanism.
Methods: Tcam-2 cells were treated with carbenoxolone (CBX) at 100 μmol/L and probenecid (PBN) 200 μmol/L. Then the intercellular fluorescence transmission was assessed by real-time fluorescence assay, the extracellular ATP concentration measured by chemi-luminescence immunoassay, the invasive and migratory abilities of the Tcam-2 cells detected by Transwell assay, and the expressions of the proteins Panx1, p-ERK1/2, ERK1/2, vimentin, MMP-9 and E-cadherin in the TM3 Leydig cells and testicular cancer Tcam-2 cells determined by Western blot.
Sichuan Da Xue Xue Bao Yi Xue Ban
May 2018
Nan Fang Yi Ke Da Xue Xue Bao
June 2018
Objective: To investigate the effect of acupuncture pretreatment at specific acupoints on action potential of cerebellar Purkenje cells in rats early after cerebral ischemia.
Methods: Forty male SD rats were randomized into control group, ischemia group, acupuncture pretreatment group and acupuncture pretreatment plus ischemia group. The rats in acupuncture groups received acupuncture pretreatment at Baihui and bilateral Zusanli twice daily for 7 consecutive days, after which brain slices were prepared and perfused at a lowered rate to simulate in vivo ischemic stroke.
Limited by the tiny structure of axons, the effects of these axonal hyperpolarizing inputs on neuronal activity have not been directly elucidated. Here, we imitated these processes by simultaneously recording the activities of the somas and proximal axons of cortical pyramidal neurons. We found that spikes and subthreshold potentials propagate between somas and axons with high fidelity.
View Article and Find Full Text PDFObjective: To compare the clinical utility of serum and cerebrospinal fluid (CSF) procalcitonin (PCT) for the diagnosis of bacterial meningitis (BM) among patients with suspected meningitis.
Methods: Patients with meningitis-like symptoms (n=120), admitted to the Second People's Hospital of Wuxi or the Changhai Hospital of Shanghai between January 2011 and December 2013, were prospectively and consecutively enrolled in this study. BM was finally diagnosed by CSF culture, Gram staining, quantitative polymerase chain reaction (qPCR), and treatment response.