The association between unilateral high-riding vertebral artery and atlantoaxial joint morphology: a multi-slice spiral computed tomography study of 396 patients and a finite element analysis.

Background Context: A high-riding vertebral artery (HRVA) can deviate too medially, too posteriorly, or too superiorly to allow the safe insertion of screws. However, it is unknown whether the presence of a HRVA is associated with morphological changes of the atlantoaxial joint.

Purpose: To investigate the association between HRVA and atlantoaxial joint morphology in patients with and without HRVA.

Adsorbable and self-supported 3D AgNPs/G@Ni foam as cut-and-paste highly-sensitive SERS substrates for rapid in situ detection of residuum.

We have proposed a synthetic approach to produce self-supported and bendable surface-enhanced Raman scattering (SERS)-based 3D chemical sensors with high adsorptivity. Such 3D substrates consist of foam-like graphene macrostructures obtained by template-directed chemical vapour deposition on nickel foams (interconnected 3D scaffold of nickel) and uniform and high-density Ag nanoparticles wrapping around the foam graphene, via seed-mediated in situ growth process. Such 3D AgNPs/G@Ni foam substrates show high-quality SERS performance in terms of Raman signal reproducibility and sensitivity for the analyte, resulting from the high density and homogeneity of "hot spots" on AgNPs/G@Ni foam, multiple cascaded amplication (localized surface plasmon mode and optical standing waves or optical refraction) of incident laser to the 3D foam structures and powerful support from nickel scaffold.

High-performance SERS substrate based on hybrid structure of graphene oxide/AgNPs/Cu film@pyramid Si.

We present a novel surface-enhanced Raman scattering (SERS) substrate based on graphene oxide/silver nanoparticles/copper film covered silicon pyramid arrays (GO/AgNPs/PCu@Si) by a low-cost and simple method. The GO/AgNPs/PCu@Si substrate presents high sensitivity, good homogeneity and well stability with R6G molecules as a probe. The detected concentration of Rhodamine 6 G (R6G) is as low as 10 M.

[Study on artificial compound feed for Buthus martensii].

Objective: To solve the problem that the single feed causing malnutrition, extension of the life cycle and low survival rates of Buthus martensii.

Methods: By using Minitab (R) 15.1.

[Study on spatial distribution pattern and seasonal distribution of Buthus martensii in yan'an].

Objective: To study population dynamics of Buthus martensii and its wild spatial distribution pattern in Yan'an.

Methods: Every month, the 1st, 10th and 20th day were designated to collect Buthus martensii samples and count on the collection by using expansion pattern target, Taylor power law and Lwao m-x regression analysis.

Results: Taylor power law regression equation was Ig S2 = 1.

[Interferon-alpha-2b induces molecular responses of patients with polycythemia vera and its post-polycythemic myelofibrosis].

To evaluate the efficacy and safety of interferon-alpha-2b (IFN-α-2b) in polycythemia vera patients(PV patient) with or without post-polycythemic myelofibrosis (post-PV MF), 30 patients with mutated JAK2V617F were enrolled in this study, from which 29 patients were evaluable. The percentage of mutated JAK2V617F allele (V617F%) was evaluated by real-time polymerase chain reaction (RT-PCR) before and after treatment with IFN-α-2b. The correlation of V617F allele burden with the major clinical outcomes was studied.

[Relation of HLA-DRB1*15 with pathogenesis in 162 childhood cases of acute lymphoblastic leukemia].

To unravel the relation of HLA-DRB1*15 with childhood acute lymphoblastic leukemia (ALL), 162 childhood patients with ALL were selected for this investigation. 1 000 normal umbilical cord blood samples were used as control.HLA-DRB1*15 and HLA-DRB5* were typed by polymerase chain reaction (PCR) analysis.

[Clinical study on relationship between JAK2 V617F mutation and chronic myeloproliferative disorders].

Objective: To investigate JAK2V617F mutation and its clinical significance in patients with chronic myeloproliferative disorders (cMPD).

Methods: A retrospective study was performed on 523 cMPD patients diagnosed according to the current World Health Organization (WHO) criteria. Allele-specific PCR (ASP) was used to identify JAK2V617F mutation, the mutation status was analyzed by PCR-RFLP, and the results were confirmed by sequence analysis.

[Studying of clinical and laboratory features of chronic eosinophilic leukemias /hypereosinophilic syndrome].

Objective: To investigate the clinical and laboratory features of chronic eosinophilic leukemias (CEL) and hypereosinophilic syndrome (HES).

Methods: The clinical manifestations, laboratory parameters were retrospectively analyzed in 20 patients with HES/CEL. Detection of the FIP1L1-PDGFRA fusion gene was performed by nested RT-PCR.

[Monitoring IgH levels in patients with B-cell malignancy by real-time quantitative PCR after hematopoietic stem cell transplantation and its significance].

The study was purpose to evaluate the value of real time quantitative-PCR for monitoring IgH level in patients with B-cell malignancy after hematopoietic stem cell transplantation (HSCT). Quantification of IgH levels was performed on bone marrow mononuclear cells from 9 patients with B-cell malignancy before and after HSCT by PCR using the consensus JH TaqMan probe in combination with an allele-specific oligonucleotide (ASO) upstream primer. The IgH levels was normalized by control gene GAPDH.

[Long-term therapeutic outcome of patients with acute promyelocytic leukemia].

Objective: To analyze the long-term therapeutic outcome of patients with acute promyelocytic leukemia(APL).

Methods: Newly diagnosed APL patients were treated with ATRA as induction therapy followed by 3-4 courses of combined consolidation chemotherapy and 2 year maintenance therapy with ATRA and 6-MP + methrotrexate, alternatively. Patients were regularly monitored with nested RT-PCR for PML-RARalpha fusion transcript at the end of consolidation chemotherapy and in the following 4 to 5 years.

[Detection of CBFbeta/MYH11 fusion transcripts and study of the mechanism of leukemogenesis of CBFbeta/SMHHC fusion protein].

Objective: To explore CBFbeta/MYH11 fusion transcripts and its expressing product CBFbeta/SMHHC fusion protein in mechanism of leukemogenesis.

Methods: CBFbeta/MYH11 fusion transcripts were detected by combined RT-PCR with sequencing. Transcription assays were examined using pM-CSFR-Luc as reporting plasmid, and subcellular localization of encoding proteins were assayed by double immunofluorescent staining and Western blot.

[In vitro expansion of cord blood megakaryocyte progenitor].

Objective: To expand cord blood megakaryocyte progenitor cells in vitro.

Methods: Cord blood CD34+ cells were selected by magnetic cell sorting (MACS), and thrombopoietin (TPO), interleukin-11 (IL-11), and heparin were used in the expansion system of megakaryocyte progenitor. The expansion efficiency was measured by fluorescence-activated cell sorting (FACS) using the megakaryocytic specific monoclonal antibodies (CD34+, CD41a+, CD61+, CD34+CD41a+, CD41a+CD61+) and colony-forming units-megakaryocyte (CFU-MK) analysis.

[Activation of auto-mesenchymal stem cells of skeletal muscle by bone morphogenetic protein for rescuing bone marrow failure].

Objective: To explore the effect of bone morphogenetic protein (BMP) to activate mesenchymal stem cells of skeletal muscle for rescuing bone marrow failure.

Methods: The study was performed on lethal rat acute aplastic anemia model induced by combined 5-fluorouracil (5-FU) and busulfan. The rh-BMP-2 was implanted into the thigh muscle of the rats at 3 days before aplastic anemia was induced.