Spatial transcriptome and single-cell RNA sequencing reveal the molecular basis of cotton fiber initiation development.

Recent advances in single-cell transcriptomics have greatly expanded our knowledge of plant development and cellular responses. However, analyzing fiber cell differentiation in plants, particularly in cotton, remains a complex challenge. A spatial transcriptomic map of ovule from -1 DPA, 0 DPA, and 1 DPA in cotton was successfully constructed, which helps to explain the important role of sucrose synthesis and lipid metabolism during early fiber development.

Erratum: Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma: Erratum.

[This corrects the article DOI: 10.7150/thno.90322.

AMPK activation by hepatitis E virus infection inhibits viral replication through attenuation of autophagosomes and promotion of innate immunity.

Hepatitis E virus (HEV) infection is generally asymptomatic or leads to acute and self-limiting hepatitis. The mechanisms orchestrating such an infection course remain to be elucidated. AMP-activated protein kinase (AMPK) is a pivotal cellular sensor for maintaining metabolic homeostasis.

Efficacy and safety of sintilimab plus bevacizumab and CAPOX as first-line treatment for patients with RAS-mutant, microsatellite stable, metastatic colorectal cancer.

Background: This research aimed to assess the efficacy and safety of combining sintilimab with bevacizumab, oxaliplatin, and capecitabine as a primary therapy for patients with RAS-mutated, microsatellite stable (MSS), and metastatic colorectal cancer (mCRC).

Methods: In this prospective, open-label, single-arm, phase II trial, eligible patients received up to 8 cycles of capecitabine and oxaliplatin/bevacizumab plus sintilimab, followed by maintenance therapy with capecitabine, bevacizumab, and sintilimab every three weeks until disease progression. Treatment response was evaluated every 2 cycles (6 weeks) according to the Response Evaluation Criteria in Solid Tumors version 1.

Rhodium-Catalyzed Asymmetric Access to P-Chiral Triarylphosphine Oxides via Insertion of P-H Bonds into Carbenes.

While significant progress has been made in enantioselective insertion of carbenes into heteroatom-hydrogen bonds, this chemistry is predominantly restricted to construction of carbon-based chirality. The asymmetric insertion of carbenes into P-H that delivers P-chiral products remains untouched. We herein report rhodium(I)-catalyzed enantioselective synthesis of P-chiral triarylphosphine oxides via asymmetric C-P coupling of a secondary phosphine oxide (SPO) with diazonaphthoquinone.

Efficient Electromyography-Based Typing System: Towards a Novel Approach to HCI Text Input.

While electromyography (EMG) excels in static gesture recognition and medical diagnoses, its application to real-time interactions like typing is hampered by the difficulty of reconciling continuous EMG signals with discrete output decisions. This paper presents a novel EMG typing system that tackles this challenge by utilizing Connectionist Temporal Classification (CTC) for efficient continuous recognition and a parallel inference approach for improved accuracy. This system enables rapid feedback and accurate word recognition, with experimental results demonstrating a character error rate of 3.

Achieving High-Temperature Resistant Afterglow by Modulating Dual-Mode Emission of Organic Emitters through Defects Engineering.

Dual-mode afterglow, integrating ultralong phosphorescence and delayed fluorescence, offers promising opportunities for developing high-temperature resistant luminescent materials. However, there is an obvious gap between the emission lifetime of delayed fluorescence and phosphorescence. In this work, a dehydration-induced doping strategy is proposed to achieve high-temperature resistant afterglow by balancing phosphorescence and delayed fluorescence lifetime.

A novel and promising therapeutic approach for treating pancreatic cancer: Nectin‑4‑targeted antibody‑drug conjugates alone or combined with autophagy inhibitors.

Antibody‑drug conjugates (ADCs) are rapidly advancing the treatment of solid tumors, and Nectin‑4‑targeted ADCs have been approved by the FDA to treat certain cancers. Although Nectin‑4 is overexpressed in the tissues of patients with pancreatic cancer, whether Nectin‑4‑targeted ADCs can effectively treat pancreatic cancer remains unclear. The present study evaluated the therapeutic effects and mechanisms of Nectin‑4‑targeted ADCs in pancreatic cancer.

Transcriptome‑wide excavation and expression pattern analysis of the NAC transcription factors in methyl jasmonate- and sodium chloride-induced Glycyrrhiza uralensis.

The NAC family is among the most extensive sets of plant-exclusive transcription factors (TFs), which are crucial for various plant development and stress response processes. Although a growing number of studies have been carried out on the NAC family in different species, it has not been characterized in Glycyrrhiza uralensis. To thoroughly understand the effects of methyl jasmonate (MeJA) and sodium chloride (NaCl) inductions on NAC TFs and investigate the underlying regulatory mechanism of NAC TFs in response to MeJA and NaCl on the biosynthesis of metabolites, we used transcriptome sequencing combined with qRT‒PCR to explore differential gene expression.

PnNAC03 from Panax notoginseng functions in positively regulating saponins and lignin biosynthesis during cell wall formation.

PnNAC03 positively regulates saponin biosynthesis and lignin accumulation during secondary cell wall formation by directly binding to the promoters of key saponin and lignin biosynthetic genes. The NAC transcription factor family plays a crucial role in the regulation of secondary metabolites biosynthesis. Saponins are the major bioactive compounds for Panax notoginseng, which is a world-globally recognized medicinal plant and possesses multiple pharmacological activities.

Adverse prognosis gene expression patterns in metastatic castration-resistant prostate cancer.

Metastatic castration-resistant prostate cancer (mCRPC) is a heterogeneous disease. Several studies have identified transcriptional subtypes of mCRPC, but comprehensive analysis of prognostic gene expression pathways has been limited. Therefore, we aggregated a cohort of 1012 mCRPC tissue samples from 769 patients and investigated the association of gene expression-based pathways with clinical outcomes and intrapatient and intratumor heterogeneity.

CuPt Alloy Enabling the Tandem Catalysis for Reduction of HCOOH and NO to Urea at High Current Density.

The formation of urea by electrocatalytic reduction of C-reactants and NO is an attractive way to store renewable electricity, close the carbon cycle, and eliminate nitrate contaminants from wastewater. Involving insufficient supply of C reactants and multiple electron transfers makes the reaction difficult to achieve high Faraday efficiency and high yield at high current density. Here, a urea synthesis approach is presented via electrocatalytic reductive coupling between liquid HCOOH and NO on copper foam (CF) loaded CuPt catalyst with optimized ratios.

Intraoperative Low-Dose Glucocorticoids in Surgical Patients With Abdominal Sepsis: A Multicenter Retrospective Cohort Study.

Background And Aims: Abdominal sepsis refers to a severe and potentially life-threatening condition characterized by the presence of infection, inflammation, and tissue damage within the abdominal cavity. Glucocorticoids (GCs) play an important role in regulation of the host immune and inflammation responses involved in sepsis and surgery. This study aimed to investigate the potential impact of intraoperative GCs administration on the clinical outcome of surgical patients with abdominal sepsis.

Charlson syndrome index predicted survival in pancreatic cancer patients received immunotherapy.

Objective: The Charlson Comorbidity Index (CCI) is a widely utilized assessment tool for evaluating the mortality rate among patients with chronic diseases and tumors. Currently, there is a dearth of research investigating the correlation between CCI and survival rates in advanced pancreatic cancer patients received immunotherapy. Therefore, this study aims to elucidate the association between CCI and survival rates in real-world settings for pancreatic cancer patients received immunotherapy.

[Research progress of acupuncture regulating enteric nervous system in Parkinson's disease].

Parkinson's disease (PD) is a neuro-degenerative disease that is common in middle-aged and elderly people, and its incidence is increasing year by year. Studies have shown that pathological products produced by a series of pathological cascade reactions in the enteric nervous system (ENS) of PD patients travel to the brain through the vagus nerve pathway in the microbial-gut-brain axis, and ultimately lead to the occurrence and development of PD. Acupuncture plays an important role in interfering with pathological products of ENS, restoring ecological balance of intestine and delaying PD process.

Genomic characterization of healthcare-associated ST5 MRSA causing severe pseudomembranous enteritis in intensive care unit.

Background: Methicillin resistant Staphylococcus aureus (MRSA) is one of the main causes of hospital-acquired infections, but the diagnosis of MRSA pseudomembranous enteritis has faded in recent years. Here, we reported a pseudomembranous enteritis case in a young male patient caused by ST5 MRSA.

Methods: Clinical data of the patient were collected from medical records.

Association of blood pressure parameters on early neurological deterioration in patients with mild stroke and large vessel occlusion following medical management.

Objective: To explore the association between blood pressure (BP) metrics and early neurological deterioration of ischemic origin (END) in patients with mild stroke and large vessel occlusion (LVO) undergoing best medical management (BMM).

Methods: Data were collected from consecutive patients with mild stroke and LVO treated with BMM from January 2019 to December 2023. Admission systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) and 24-h SBP variability were calculated.

Diet and Living Environment as Novel Etiological Factors for Melasma: The Results Form a Retrospective Case-Control Study of 150 Chinese Patients.

Background: Melasma, a condition with complex and multifactorial pathogenesis, has traditionally been linked to factors such as ultraviolet (UV) exposure, hormonal changes, and genetic predisposition. Despite societal advances and shifts in lifestyle, updates on causal factors remain limited. Our study aims to address this gap by investigating emerging pathogenic factors that differ from those conventionally reported.

Epidemiological and Spatiotemporal Clustering Analysis of Human Brucellosis - China, 2019-2023.

What Is Already Known About This Topic?: The number of reported cases of human brucellosis significantly increased from 45,046 (3.25/100,000) in 2019 to 70,439 (4.99/100,000) in 2023.

Identification of serum phosphatidylinositol glycan anchor biosynthesis class U protein as diagnostic biomarker for breast cancer.

Background: Finding markers of breast cancer that are strong specificity and high. Sensitivity is important. The expression of phosphatidylinositol U（PIGU） protein is increased in a variety of tumor cells, but the role of its secreted fragment in breast cancer is lacking.

Anticoagulants utilization in eight hospitals within the Luzhou region from 2019 to 2023.

Background: With the increasing utilization of anticoagulants, the selection of appropriate anticoagulants has emerged as a significant quandary. The objective of this study was to evaluate recent trend in the utilization and expenditure of anticoagulants within a specific region, aiming to provide valuable insights into the optimal choice of anticoagulants across other healthcare facilities.

Methods: The utilization of anticoagulants was retrospectively analyzed.

Analysis and validation of serum biomarkers in brucellosis patients through proteomics and bioinformatics.

Introduction: This study aims to utilize proteomics, bioinformatics, and machine learning algorithms to identify diagnostic biomarkers in the serum of patients with acute and chronic brucellosis.

Methods: Proteomic analysis was conducted on serum samples from patients with acute and chronic brucellosis, as well as from healthy controls. Differential expression analysis was performed to identify proteins with altered expression, while Weighted Gene Co-expression Network Analysis (WGCNA) was applied to detect co-expression modules associated with clinical features of brucellosis.

Intraspecific identification and genetic diversity analysis of Paeonia lactiflora based on chloroplast genes rpoB and psbK-psbI.

Background: Paeonia lactiflora Pall., a member of Paeoniaceae family, is a medicinal herb widely used in traditional Chinese medicine. Chloroplasts are multifunctional organelles containing distinct genetic material.

Using peer narrative support to promote return to work for cancer survivors: a protocol study of action research.

Background: Chinese cancer survivors are not doing well in returning to work. Peer support, as an external coping resource to help cancer survivors return to work, brings together members of the lay community with similar stressors or problems for mutual support. Peer volunteers have not received systematic training, so inappropriate language in the support process can often cause secondary damage to both the peer and the cancer survivor.

Identification of germplasm resources based on metabolomics and DNA barcoding.

Introduction: radix et rhizoma (Danshen) is a crucial medicinal material for treating cardiovascular and cerebrovascular diseases. However, the presence of adulterants and intraspecific variability poses challenges to its clinical safety.

Methods: This study collected samples of from various regions and commonly encountered adulterants.