Histochemical assessment of the femora of Spontaneously Diabetic Torii-Lepr (SDT-fa/fa) rats that mimic type II diabetes.

Objective: To elucidate the mechanisms underlying diabetic osteoporosis, we conducted a comprehensive histological examination of the femora of Spontaneously Diabetic Torii-Lepr (SDT-fa/fa) rats, an established model of obesity-related type 2 diabetes.

Materials And Methods: Femora from 12 30-week-old male SDT-fa/fa rats and age-matched Sprague-Dawley (SD) rats (controls) were used for detailed histochemical analyses, including tartrate-resistant acid phosphatase (TRAP), cathepsin K, alkaline phosphatase (ALP), phosphoethanolamine/ phosphocholine phosphatase 1 (PHOSPHO1), dentin matrix protein (DMP)-1, matrix extracellular phosphoglycoprotein (MEPE), sclerostin, osteocalcin staining, silver impregnation, von Kossa staining, and micro-computed tomography (CT).

Results: Micro-CT and hematoxylin-eosin staining demonstrated significantly reduced trabecular bone volume in the femoral metaphyses of SDT-fa/fa rats.

Individual and joint associations between sleep duration and physical activity with cognitive function: A longitudinal analysis among middle-aged and older adults in China.

Introduction: Studies using cross-sectional data or with a short follow-up period fail to distinguish whether the associations between sleep duration and physical activity with cognitive function result from reverse causation.

Methods: The longitudinal study examined the individual and joint associations, with specific temporality, between sleep duration and physical activity with cognitive function, using time-lagged linear mixed models and generalized additive mixed models.

Results: A total of 14,694 participants aged ≥ 50 years were included, with an average lagged time of 4.

Epidermal stem cell-derived exosomes improve wound healing by promoting the proliferation and migration of human skin fibroblasts.

Background: Epidermal stem cells (ESCs) are primarily located in the basal layer of the epidermis and play a crucial role in wound healing. ESCs-derived exosomes (ESCs-Exo) are emerging as promising candidates for skin regeneration and wound healing. However, the underlying mechanisms remain unclear.

The case report of the impact of coach self-supportive behaviors on the communication effectiveness with depressed athletes: The mediating role of intrinsic motivation.

Rationale: Coach autonomy-supportive behaviors are a crucial factor in the communication effectiveness with athletes experiencing depressive moods. Therefore, clarifying the relationship between coach autonomy-supportive behaviors and the communication effectiveness with athletes experiencing depressive moods is of significant importance for promoting effective communication between coaches and athletes. This study employs in-depth interviews, questionnaires, and stepwise regression analysis, using purposive sampling to investigate coaches and athletes experiencing depressive moods from various regions, levels, genders, and sports in China.

TEAD3 and TEAD4 play overlapping role in bovine preimplantation development.

During mammalian preimplantation development, the transition from morula to blastocyst is a critical biological event. This process involves polarization and initial specification of lineages, regulated by various transcription factors that have been extensively studied in mice. Our single-cell RNA sequencing analyses revealed that TEAD3 is specifically expressed in the trophectoderm cells of bovine preimplantation embryos, unlike in mice.

Study on the fusion of improved YOLOv8 and depth camera for bunch tomato stem picking point recognition and localization.

When harvesting bunch tomatoes, accurately identifying certain fruiting stems proves challenging due to their obstruction by branches and leaves, or their similarity in colour to the branches, main vines, and lateral vines. Additionally, irregularities in the growth pattern of the fruiting pedicels further complicate precise picking point localization, thus impacting harvesting efficiency. Moreover, the fruit stalks being too short or slender poses an obstacle, rendering it impossible for the depth camera to accurately obtain depth information during depth value acquisition.

Development and Validation of the Predictive and Prognostic ChemoResist Signature in Resected Pancreatic Ductal Adenocarcinoma: Multicenter Study.

Objective: To develop and validate a signature to precisely predict prognosis in pancreatic ductal adenocarcinoma (PDAC) undergoing resection and adjuvant chemotherapy.

Summary Background Data: PDAC is largely heterogeneous, and responds discrepantly to treatment.

Methods: 551 consecutive patients with PDAC from 3 tertiary centers were initially enrolled.

Genetic profile in primary tumor tissue of advanced lung adenocarcinoma patients with adrenal metastasis.

The aim of this study was to examine the genomic characteristics and explore the molecular mechanisms underlying adrenal metastases in lung adenocarcinoma. 57 patients diagnosed with lung adenocarcinoma (LUAD) and adrenal metastases (AM) were enrolled, alongside 33 controls diagnosed with non-adrenal metastases (non-AM) at the time of diagnosis. The primary lung cancer tissue sample were analyzed using next-generation sequencing.

Radiolabelled anti-PD-L1 peptide PET/CT in predicting the efficacy of neoadjuvant immunotherapy combined with chemotherapy in resectable non-small cell lung cancer.

Background: This study aimed to evaluate the predictive value of baseline PD-L1 targeted peptide Ga-NOTA-WL12 PET/CT in neoadjuvant immunotherapy combined with chemotherapy of resectable NSCLC.

Methods: Patients with resectable NSCLC (n = 20) enrolled in this prospective study received baseline paired Ga-NOTA-WL12 PET/CT and F-FDG PET/CT. After 2-4 cycles of toripalimab plus nab-paclitaxel and cisplatin, surgery was performed if R0 resection was available.

Pb alleviates As mobilization during the biological reductive dissolution of Pb-As jarosite.

The biological dissolution of jarosite accelerates As mobilization in acid mine drainage environments, which can be influenced by coexisting metals. However, few studies have focused on the effects of coexisting Pb on this biogeochemical process. Here, we investigated the behavior of As during the reductive dissolution of Pb-As jarosite (PbFe(SO, AsO)(OH)) by a sulfate-reducing bacterium (SRB) of Desulfovibrio desulfuricans.

Construction of Pt─O Sites on Pt Nanoclusters in Silicalite-1 Zeolite for Efficient Catalytic Oxidation of Hydrogen Isotope Gases.

The construction, use, and maintenance of tritium-related equipment will inevitably produce tritium-containing radioactive waste gas, and the production of efficient catalysts for tritium removal remains a difficult problem. Herein, silicalite-1 zeolite with entrapped Pt nanoclusters is skillfully post-oxidized at an appropriate temperature, building highly active Pt─O sites on the nanoclusters to achieve efficient oxidation of hydrogen isotopes at low temperatures. The designed Pt─O sites can directly participate in the oxidation reaction of hydrogen isotopes.

Branched-chain amino acids deficiency promotes diabetic cardiomyopathy by activating autophagy of cardiac fibroblasts.

More than half of the patients with type II diabetes mellitus (T2D) develop diabetic cardiomyopathy (DCM). Glycemic control alone cannot effectively prevent or alleviate DCM. Herein, we concentrated on the variations in levels of metabolites between DCM and T2D patients without cardiomyopathy phenotype.

Impact of COVID-19 on acute angle-closure attack: A retrospective study.

To systematically review the characteristics of patients experiencing acute angle closure (AAC) attacks during the COVID-19 outbreak in Beijing. Patients with AAC attacks during the COVID-19 epidemic and those in the same period the following year were recruited. Demographic characteristics, ocular biometry, ocular signs, sequential relationships, and the prognosis of operative management outcome were recorded and compared between the 2 groups.

Preparation of polyclonal antibody to CHOP protein and its application in heat stress of chickens.

Heat stress (HS) is a stress response of organisms to temperature changes, which can result in organ damage and increased chicken mortality in high-temperature environments. The CHOP protein, also known as GADD 135, plays a crucial role in endoplasmic reticulum stress. However, there are fewer studies related to whether CHOP proteins are involved in heat stress-induced organ damage.

A novel T2-weighted series-based modified vertebral bone quality score for evaluating bone mineral density.

Objective: To evaluate the modified vertebral bone quality (VBQ) method on the magnetic resonance imaging (MRI) T1-weighted (T1w), T2-weighted (T2w), and fat suppression (FS) series in evaluating bone mineral density (BMD) for patients with degenerative lumbar disease.

Methods: We reviewed preoperative data of postmenopausal female patients aged ≥ 50 years hospitalized to undergo lumbar surgery for degenerative lumbar disease with available MRI and dual-energy X-ray absorptiometry (DEXA). Patients were categorized into three groups according to T-score.

Histological assessments for anabolic effects in teriparatide/abaloparatide administered rodent models.

Parathyroid hormone (PTH) is thought to induce remodeling-based bone formation by promoting osteoclastic activity, a process known as cellular coupling. Our research has shown that the frequency of PTH administration affects trabecular number and thickness. High-frequency PTH administration induced remodeling-based bone formation, while less frequent administration induced both remodeling-based and modeling-based bone formation.