Establishment of a Real-Time Reverse Transcription Recombinase-Aided Isothermal Amplification (qRT-RAA) Assay for the Rapid Detection of Bovine Respiratory Syncytial Virus.

Background: Bovine respiratory syncytial virus (BRSV) is a significant cause of bovine respiratory disease, resulting in significant losses to the cattle industry. For rapid detection of BRSV, a real-time recombinase-aided isothermal amplification assay (qRT-RAA) based on the gene of BRSV was developed in this study.

Results: The developed qRT-RAA assay showed good exponential amplification of the target fragment in 20 min at a constant temperature of 39 °C.

Control of Postharvest Green Mold in Citrus by the Antimicrobial Peptide BP15 and Its Lipopeptides.

This study examined the efficacy and mechanisms of action of the antimicrobial peptide BP15 and its lipopeptides, HBP15 and LBP15, against , the primary causative agent of green mold in citrus fruits. The findings revealed that all three antimicrobial peptides markedly inhibited the spore germination and mycelial growth of , with minimum inhibitory concentrations (MICs) of 3.12 μM for BP15, HBP15, and LBP15.

Serum proteome profiling of plateau acclimatization in men using Olink proteomics approach.

Plateau acclimatization involves adaptive changes in the body's neurohumoral regulation and metabolic processes due to hypoxic conditions at high altitudes. This study utilizes Olink targeted proteomics to analyze serum protein expression differences in Han Chinese individuals acclimatized for 6 months-1 year at 4500 and 5300 m altitudes, compared to those residing at sea level. The objective is to elucidate the proteins' roles in tissue and cellular adaptation to hypoxia.

Lignin and copper nanocomposite coating for antibacterial mask.

Lignin is a natural phenolic polymer characterized with renewable, sustainable and biocompatible, but yet remain underutilized. In the post-pandemic era, people are conventionally reusing mask but without any disinfections to prevent infection of virus in public places, which could lead to accumulation of bacteria and secondary infections. The development of antibacterial mask from lignin would simultaneously address the hygiene issues of used mask due to microbe accumulation and provide novel approach for lignin valorization.

Physalins and neophysalins from the calyx of Physalis alkekengi: Structures and anti-inflammatory efficacy.

To explore potential anti-inflammatory lead compounds, ten new physalin steroids, including three neophysalins (1, 4, and 9) and seven physalins (2, 3, 5-8, and 10), along with eleven known analogs, were isolated from an ethanol extract of the calyx of Physalis alkekengi. The new structures were rigorously determined through comprehensive HRESIMS, 1D/2D-NMR, and X-ray diffraction analysis. Among these compounds, 1 was identified as a new 1,10-seco-neophysalin, and 2 was identified as a new 11,15-cyclo-9,10-seco-physalin characterized by an aromatic A-ring.

High-Efficiency Fluorescent-Coupled Optical Fiber Passive Tactile Sensor with Integrated Microlens for Surface Texture and Roughness Detection.

Integrating ZnS:Cu@AlO/polydimethylsiloxane (PDMS) flexible matrices with optical fibers is crucial for the development of practical passive sensors. However, the fluorescence coupling efficiency is constrained by the small numerical aperture of the fiber, leading to a reduction in sensor sensitivity. To mitigate this limitation, a microsphere lens was fabricated at the end of the multimode fiber, which resulted in a 21.

To kill or to piggyback: Switching of viral lysis-lysogeny strategies depending on host dynamics.

Viruses wield significant influence over microbial communities and ecosystem function in marine environments. However, the selection of viral life strategies and their impacts on microbial communities remains enigmatic. In this study, we utilized a large-scale macrocosm, established using water samples from a marine coastal region, to enable community-level investigation.

DEK::AFF2 Fusion Sinonasal and Skull Base Nonkeratinizing Squamous Cell Carcinoma: A Clinical Outcome Study Compared With Conventional Sinonasal Squamous Cell Carcinoma.

DEK::AFF2 fusion nonkeratinizing squamous cell carcinoma (NKSCC) is an emerging entity in the sinonasal tract, temporal bone, and skull base. However, the clinical behavior of these tumors has not been well studied. Here, we report the largest cohort of DEK::AFF2 carcinomas to determine if morphology, mitotic rate, and/or Ki-67 IHC are associated with patient outcomes, including a comparison with high-risk human papillomavirus (HPV)-associated and independent patients.

Correlation analysis of key genes and immune infiltration in visceral adipose tissue and subcutaneous adipose tissue of patients with type 2 diabetes in women.

Immune cell infiltration into adipose tissue (AT) is a key factor in type 2 diabetes (T2DM). However, research on the impact of fat distribution on immune cells and immune responses in women is still lacking. This study used enrichment, protein-protein interaction network, immune cell infiltration, and correlation analysis to compare the similarities and differences between the transcriptome data of visceral AT (VAT) and subcutprotein-proteinaneous AT (SAT) obtained from the omprehensive database of gene expression in women with non-T2DM and T2DM.

Dual-mode colorimetric and chemiluminescence aptasensor for organophosphorus pesticides detection using aptamer-regulated peroxidase-like activity of TA-Cu.

The residues of organophosphorus pesticides (OPs) in food pose a huge threat to human health. Therefore, the development of detection methods with simple design and high sensitivity is urgently needed. Here, a colorimetric/chemiluminescence (CL) dual-mode aptasensor strategy with high selectivity and sensitivity for detecting Parathion-methyl (PM) was designed based on aptamer-regulated nanozyme activity.

Indium Mediated Reformatsky-Type Allylation of N--Butanesulfinyl Iminoester with 1,3-Butadiene.

This study presents the indium-mediated three-component radical Reformatsky-type allylation of --butanesulfinyl iminoester with 1,3-butadiene. This novel approach offers a rapid synthesis pathway to valuable homoallylic noncanonical amino acids, demonstrated with over 30 examples showing nice regio- and diastereoselectivity. Mechanism studies revealed that allylindium complexes served as key intermediates, formed through a single-electron reduction of allylic radicals by Indium species.

A chromosome-scale reference genome assembly for Triplophysa lixianensis.

In this study, we constructed a chromosome-scale reference genome assembly for Lixian plateau loach, Triplophysa lixianensis, by integration of MGI short-read, PacBio HiFi long-read and Hi-C sequencing technologies. A 668-Mb haplotypic genome assembly was obtained for a female T. lixianensis, and 98.

Zinc-based radioenhancers to activate tumor radioimmunotherapy by PD-L1 and cGAS-STING pathway.

Radiotherapy and immunotherapy have already become the primary form of treatment for non-small-cell lung cancer (NSCLC), but are limited by high radiotherapy dose and low immune response rate. Herein, a multi-pronged strategy using a radio-immuno-enhancer (ZnO-Au@mSiO) is developed by inducing tumor cells apoptosis and reprograming the immunosuppressive tumor microenvironment (TME). The radio-immuno-enhancer employed Au as a radiosensitizer, transition Zn ions as immune activators, which not only tremendously enhances the anti-proliferative activity of radiotherapy toward cancer cells, but also activates the immune response with multi-targets to let "exhausted" T cells "back to life" by triggering immunogenic cell death (ICD), immune checkpoint blockade (ICB) that target PD-1/PD-L1 and cGAS-STING under X-ray irradiation with a low dosage.

[Anti-tumor mechanism of traditional Chinese medicine via Hedgehog signaling pathway: a review].

The Hedgehog(Hh) signaling pathway has the functions of improving embryogenesis and maintaining tissue homeostasis. By influencing the tumor microenvironment, intervening in cell apoptosis, and regulating angiogenesis, it is pivotal in the occurrence, progression, and recovery of various tumors. Therefore, targeting and inhibiting the abnormal activation of the Hh pathway has become a potential research strategy for alleviating and treating cancer.

Selective acceleration and inhibition of crystal growth of glass carbamazepine by low-concentration poly(ethylene oxide):effects of drug polymorph.

Drug polymorphism attracts considerable interest within the pharmaceutical field. Herein, we investigate the impact of low-concentration poly(ethylene oxide) (PEO) on the crystal growth of carbamazepine (CBZ) polymorphs in the glassy state. The addition of 3%(w/w) PEO increases 5.

DNA-Assisted Assays for Studying Lipid Transfer Between Membranes.

Extended-synaptotagmins (E-Syts) are proteins located on the endoplasmic reticulum (ER) that tether the ER to the plasma membrane (PM) and regulate their lipid homeostasis via its lipid transfer module, the synaptotagmin-like mitochondrial lipid-binding protein (SMP) domain. Here, we describe in vitro DNA nanostructure-assisted lipid transfer assays investigating how the SMP domain transports lipids between membranes and associates with the membranes to extract and release lipids. The lipid transfer signal was detected through fluorescence resonance energy transfer (FRET).

Design, Synthesis, and Biological Evaluation of 2-Arylaminopyrimidine Derivatives as Dual Cathepsin L and JAK Inhibitors for the Treatment of Acute Lung Injury.

Acute lung injury (ALI) is a disease characterized by pulmonary inflammation, blood barrier functional disorder, and hypoxemia. Herein, a series of 2-aminopyrimidine derivatives were synthesized. Most of them exhibited inhibitory effects on inflammatory cytokines IL-6 and IL-8 in human bronchial epithelial (HBE) cells at a concentration of 5 μM without significant cytotoxicity.

Catalysis under electric-/magnetic-/electromagnetic-field coupling.

The ultimate goal of catalysis is to control the cleavage and formation of chemical bonds at the molecular or even atomic level, enabling the customization of catalytic products. The essence of chemical bonding is the electromagnetic interaction between atoms, which makes it possible to directly manipulate the dynamic behavior of molecules and electrons in catalytic processes using external electric, magnetic and electromagnetic fields. In this tutorial review, we first introduce the feasibility and importance of field effects in regulating catalytic reaction processes and then outline the basic principles of electric-/magnetic-/electromagnetic-field interaction with matter, respectively.

Transcriptomic and proteomic sequencing unveils the role of vitamin D and metabolic flux shifts in the induction of human hepatic organoids.

Background: Hepatic organoids (HOs), validated through comparative sequencing with human liver tissues, are reliable models for liver research. Comprehensive transcriptomic and proteomic sequencing of HOs throughout their induction period will enhance the platform's utility, aiding in the elucidation of liver development's molecular mechanisms.

Methods: We developed hepatic organoids (HOs) from embryonic stem cells (ESCs) through a de novo induction protocol, mimicking the stages of fetal liver development: ESCs to definitive endoderm (DE), then to foregut (FG), hepatoblasts (HB), and finally to HOs stage 1 (HO1), culminating in self-organizing HOs stage 2 (HO2) via dissociation and re-inoculation.

The impact of perceived stress on the prognosis in patients undergoing percutaneous coronary intervention.

Objective: To assess the impact of perceived stress levels in patients undergoing percutaneous coronary intervention (PCI) on the occurrence of major adverse cardiovascular events (MACEs) within a 6-month follow-up period postprocedure.

Methods: A cohort of 339 hospitalized patients with coronary heart disease undergoing initial PCI was prospectively enrolled in the Department of Cardiology at two branches of Zhongda Hospital Affiliated to Southeast University between January 2022 and July 2022. Baseline data including demographic and clinical characteristics, along with the Chinese Perceived Stress Scale (CPSS), were collected.

Optimization of the extraction process of goji berry pectin using response surface methodology and its suitability as thickener for yogurt.

Although goji berry with many main biological activity components is a nutritional medicinal and edible plan and widespread in the northeast of China, its current development also faces a new challenge of overcapacity. So this study was largely focused to investigate the optimal extraction process conditions (extraction water proportion, acidic pH regulating reagent, adjusted pH, extraction temperature, extraction time) for goji berry with increased yield. Box Behnken experimental design (BBD) was used to model the mathematical model of the relationship between the three main influence of pivotal extraction parameters (extraction pH, extraction time and extraction temperature) selected by the single factor experiments on the responses of goji berry pectin yield (%).

Novel homozygous SPAG17 variants cause human male infertility through multiple morphological abnormalities of spermatozoal flagella related to axonemal microtubule doublets.

Male infertility can result from impaired sperm motility caused by multiple morphological abnormalities of the flagella (MMAF). Distinct projections encircling the central microtubules of the spermatozoal axoneme play pivotal roles in flagellar bending and spermatozoal movement. Mammalian sperm-associated antigen 17 (SPAG17) encodes a conserved axonemal protein of cilia and flagella, forming part of the C1a projection of the central apparatus, with functions related to ciliary/flagellar motility, skeletal growth, and male fertility.

Permeability-Engineered Compartmentation System-Enabled Digital PCR (PECS-dPCR): A Digital Platform toward Multistep Biomolecular Assays.

Droplet-based digital PCR has emerged as a powerful platform for nucleic acid-based detection. However, the formation of droplet compartments and the subsequent amplification process in oil present significant drawbacks: instability under harsh thermal conditions, high background fluorescent noise inside droplets, and major difficulty in supporting multistep assays. Alternatively, droplets made of a hydrogel, or other advanced materials, have been adopted and demonstrate promising improvement over conventional droplet-based platforms.

An Oriented Recrystallization Nucleation Mechanism of a Cold-Rolled Pure Ti with Electric-Pulse Treatment.

The effect of electric-pulse treatment (EPT) on the nucleation behavior of a cold-rolled pure Ti was investigated. The specimens are subjected to EPT and then annealed at 650 °C within 10 min. Both the electron backscatter diffraction (EBSD) and transmission electron microscope (TEM) techniques were used for detailing the microstructural evolution of the specimens at the initial stage of recrystallization processing during annealing.