A Near-Infrared Imaging System for Robotic Venous Blood Collection.

Venous blood collection is a widely used medical diagnostic technique, and with rapid advancements in robotics, robotic venous blood collection has the potential to replace traditional manual methods. The success of this robotic approach is heavily dependent on the quality of vein imaging. In this paper, we develop a vein imaging device based on the simulation analysis of vein imaging parameters and propose a U-Net+ResNet18 neural network for vein image segmentation.

Effects of saponins R and R in intervention on intestinal microbiota of aging model.

Aging brings about physiological dysfunction, disease, and eventual mortality. An increasing number of studies indicate that aging can easily lead to dysbiosis of the gut microbiota, which can further affect digestion, nerves, cognition, emotions, and more. Therefore, gut bacteria play an important role in regulating the physical functions of aging populations.

Physicochemical and antioxidant properties of pectin from Sieb.et Zucc () extracted by ultrasonic.

Pectin was extracted from Sieb. et Zucc () using the ultrasound-assisted acid method and the single acid method. The physicochemical properties, structure, and antioxidant properties of two different pectins were investigated.

Effects of saponins Rb and Re in combined intervention on immune system of aging model.

Aging is a major risk factor for the development of many pathological processes, such as reduced immunity, cancer, cardiovascular diseases or neurodegenerative diseases, while age-related chronic diseases are the most common causes of death. This paper studies the effects of saponin Rb and Re alone and combined intervention on the immune system of aging mouse models, by using 30 mg/kg Rb, 15 mg/kg Re, and Rb + Re (30 mg/kg Rb and 15 mg/kg Re (co-intervention) was used to intervene in the aging model, and immune indicators such as thymus index, spleen index, interleukin and interferon were detected to evaluate the impact of Rb and Re on immune function. The results show that Rb and Re intervention alone can increase the spleen index by 7%-12% and the thymus index by 12%-19% in the aging model.

[Research on Vessel Segmentation and Extraction Method Based on Improved 2D Gabor Features].

This study proposed a vessel segmentation method based on Gabor features. According to the eigenvector of Hessian matrix of each pixel in the image, the vessel direction of each point was obtained to set the direction angle of Gabor filter, and the Gabor features of different vessel width at each point were extracted to establish the 6D vectors of each point. By reducing the dimension of the 6D vector, the 2D vector of each point was obtained and fused with the original image G channel.

GigaMVS: A Benchmark for Ultra-Large-Scale Gigapixel-Level 3D Reconstruction.

Multiview stereopsis (MVS) methods, which can reconstruct both the 3D geometry and texture from multiple images, have been rapidly developed and extensively investigated from the feature engineering methods to the data-driven ones. However, there is no dataset containing both the 3D geometry of large-scale scenes and high-resolution observations of small details to benchmark the algorithms. To this end, we present GigaMVS, the first gigapixel-image-based 3D reconstruction benchmark for ultra-large-scale scenes.

Pollution level and risk assessment of lead, cadmium, mercury, and arsenic in edible mushrooms from Jilin Province, China.

To evaluate the pollution level of toxic elements in edible mushrooms from Jilin Province, China, the lead, cadmium, mercury, and arsenic content present in them were monitored and evaluated. A total of 610 edible mushroom samples, including fresh and dried, were collected from nine cities in the Jilin Province. The concentrations of lead, cadmium, mercury, and arsenic ranged from 0.

The values of coagulation function in COVID-19 patients.

Objective: To investigate the blood coagulation function in COVID-19 patients, and the correlation between coagulopathy and disease severity.

Methods: We retrospectively collected 147 clinically diagnosed COVID-19 patients at Wuhan Leishenshan Hospital of Hubei, China. We analyzed the coagulation function in COVID-19 patients through the data including thrombin-antithrombin complex (TAT), α2-plasmininhibitor-plasmin Complex (PIC), thrombomodulin (TM), t-PA/PAI-1 Complex (t-PAIC), prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (APTT), fibrinogen (FIB), thrombin time (TT), D-Dimer (DD), and platelet (PLT).

Simultaneous determination of succinylcholine and its metabolite in animal-derived foods by solid-phase extraction combined with ultra-performance liquid chromatography/mass spectrometry.

Rationale: Succinylcholine has been increasingly used in the theft of animals. Because of the presence of residual levels of succinylcholine in poisoned animals, it is harmful for people to eat foods derived from these animals. Therefore, a method should be immediately established to determine succinylcholine and its metabolite in animal-derived foods.

Statistical optimization of a cellulase from Aspergillus glaucus CCHA for hydrolyzing corn and rice straw by RSM to enhance yield of reducing sugar.

Objective: The unique GH5 cellulase, AgCMCase, from Aspergillus glaucus CCHA was identified and characterized as having high cellulose and straw hydrolysis activities that were thermostable, pH stable and salt-tolerant. Therefore, it is a potential straw-degradation enzyme that can release reducing sugars in industrial applications. To increase the efficiency of the AgCMCase' hydrolysis of straw to release simple sugars, response surface methodology (RSM) was introduced to optimize hydrolysis parameters such as pH, temperature, reaction time and enzyme dose.

An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.

Numerous thermostable enzymes have been reported from the hyperthermophilic archaeon KOD1, which made it an attractive resource for gene cloning. This research reported a glycosyl hydrolase (Tk-ChiA) form with dual hydrolytic activity due to the presence of three binding domains with affinity toward chitin and cellulose. The gene was cloned and expressed on GS115.

Myeloid-derived suppressor cells are essential for maintaining feto-maternal immunotolerance via STAT3 signaling in mice.

Maternal immune system tolerance to the semiallogeneic fetus is essential for a successful pregnancy; however, the mechanisms underlying this immunotolerance have not been fully elucidated. Here, we demonstrate that myeloid-derived suppressor cells play an important role in maintaining feto-maternal tolerance. A significant expansion of granulocytic myeloid-derived suppressor cells was observed in multiple immune organs and decidual tissues from pregnant mice.

Co-metabolic transformation of the neonicotinoid insecticide imidacloprid by the new soil isolate Pseudoxanthomonas indica CGMCC 6648.

A new imidacloprid (IMI) degrading bacterium Z-9 (deposited number CGMCC 6648) was isolated and identified as Pseudoxanthomonas indica by 16S rRNA gene analysis. Two metabolites were identified as olefin and 5-hydroxy IMI by liquid chromatography-mass spectrometry and nuclear magnetic resonance analysis. P.

Probing dynamics and mechanism of exchange process of quaternary ammonium dimeric surfactants, 14-s-14, in the presence of conventional surfactants.

In this Article, we investigated effects of different types of conventional surfactants on exchange dynamics of quaternary ammonium dimeric surfactants, with chemical formula C(14)H(29)N(+)(CH(3))(2)- (CH(2))(s)-N(+)(CH(3))(2)C(14)H(29)·2Br(-), or 14-s-14 for short. Two nonionic surfactants, TritonX-100 (TX-100) and polyethylene glycol (23) laurylether (Brij-35), and one cationic surfactant, n-tetradecyltrimethyl ammonium bromide (TTAB), and one ionic surfactant, sodium dodecyl sulfate (SDS) were chosen as typical conventional surfactants. Exchange rates of 14-s-14 (s = 2, 3, and 4) between the micelle form and monomer in solution were detected by two NMR methods: one-dimensional (1D) line shape analysis and two-dimensional (2D) exchange spectroscopy (EXSY).

Mixed micelles of sodium 4-decyl naphthalene sulfonate with Triton X-100 and sodium dodecyl sulfonate analyzed by 1H NMR.

The characteristics of sodium 4-decyl naphthalene sulfonate (SDNS)/Triton X-100 (TX-100) and sodium dodecyl sulfonate (SDSN)/SDNS mixed micelles with different molar ratios were studied by 1D and 2D 1H NMR. In the mixed micelle of SDNS/TX-100 the phenoxy rings of the TX-100 are embedded in the near vicinity of the alkyl chains of SDNS and its polyoxyethylene segments, but the first oxyethylene group, to which the phenoxy ring is adjacent, are located near the naphthyl rings. In the mixed micelle of the SDNS/SDSN system the sulfonate groups of SDSN are embedded in the naphthyl rings of SDNS; i.

Miniaturizable homogenous time-resolved fluorescence assay for carboxypeptidase B activity.

An epitope-unmasking, homogeneous time-resolved fluorescence (HTRF) assay has been developed for measuring carboxypeptidase B (CPB) activity in a miniaturized high-throughput screening format. The enzyme substrate (biotin-RYRGLMVGGVVR-OH) is cleaved by CPB at the C terminus, causing release of the C-terminal Arg residue. The product (biotin-RYRGLMVGGVV-OH) is recognized specifically by a monoclonal antibody (G2-10) which is labeled with Eu(3+)-cryptate ([Eu(3+)]G2-10 mAb), and the complex is detected by fluorescence resonance energy transfer using streptavidin labeled with allophycocyanin ([XL665]SA).