Publications by authors named "Shi Lihong"

The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D and 3D immunofluorescence imaging methods. The design of the Knowledge-Base is modeled after efforts in the open-source software community and includes three facets: a development platform (GitHub), static website, and service for data archiving. The Knowledge-Base facilitates the practice of open science throughout the research life cycle by providing validation data for recommended and non-recommended reagents, e.

View Article and Find Full Text PDF

Ammonia oxidation plays a vital role in regulating soil nitrogen (N) cycle in agricultural soil, which is significantly influenced by different fertilizer regimes. However, there is still need to further investigate the effects of different fertilizer managements on rhizosphere soil ammonia-oxidizing archaea (AOA) and bacteria (AOB) community in the double-cropping rice field. Therefore, the effects of different long-term (37 years) fertilizer managements on rhizosphere soil potential nitrification activity (PNA), AOA and AOB community structure, and its relationship under the double-cropping rice system in southern of China were studied in the present paper.

View Article and Find Full Text PDF

Within the intricate milieu of colorectal cancer (CRC) tissues, cancer-associated fibroblasts (CAFs) act as pivotal orchestrators, wielding considerable influence over tumor progression. This review endeavors to dissect the multifaceted functions of CAFs within the realm of CRC, thereby highlighting their indispensability in fostering CRC malignant microenvironment and indicating the development of CAFs-targeted therapeutic interventions. Through a comprehensive synthesis of current knowledge, this review delineates insights into CAFs-mediated modulation of cancer cell proliferation, invasiveness, immune evasion, and neovascularization, elucidating the intricate web of interactions that sustain the pro-tumor metabolism and secretion of multiple factors.

View Article and Find Full Text PDF

This article explores how the parent-child bond is continued in an emergent deathscape in urban China where graves designated for deceased children are being created. This new child-centered deathscape stands in stark contrast to the dominant Chinese deathscape which is embedded in the longstanding practice of ancestor worship. Based on data collected through cemetery visits and interviews with bereaved parents, this article delves into the making of this new deathscape manifested at a cemetery in urban China to demonstrate the process of bereaved parents building and maintaining bonds with their deceased children.

View Article and Find Full Text PDF

Smartphone-assisted visual assay not only expands quantitative analysis but also enhance real-time on-site sensing capabilities. Herein, lysosome-targeted dual-emissive carbon dots (L-CDs) can not only recognize Hg and SO by ratiometric fluorescence and ratiometric absorption, but also visually quantify Hg and SO by smartphone-assisted method. With monitoring of intrinsic ratiometric fluorescent variation (I/I), L-CDs are developed as an effective sensing platform for ratiometric fluorescent successive identification of Hg and SO accompanying with continuous fluorescence variation of blue, purplish pink, pink, and light yellow.

View Article and Find Full Text PDF
Article Synopsis
  • The study investigates extrachromosomal circular DNAs (eccDNAs) in breast cancer tissues using Circle-seq, revealing that eccDNAs are present in all chromosomes and are concentrated in specific breast cancer-related genes.* -
  • Researchers identified eccDNAs that contain entire genes (eccGenes) and microRNAs (eccMIRs) linked to cancer pathways, with synthetic eccMIR6748 shown to enhance miRNA expression in breast cancer cells, affecting cell behavior and tumor suppressor activity.* -
  • The findings indicate that eccDNAs contribute to breast cancer initiation and progression, presenting a new area of genomic variation that could lead to potential biomarkers and treatment options for breast cancer.*
View Article and Find Full Text PDF

Lysine succinylation (Ksu) has recently emerged as a protein modification that regulates diverse functions in various biological processes. However, the systemically and precise role of lysine succinylation in erythropoiesis remains to be fully elucidated. In this study, we noted a prominent increase of succinyl-CoA and lysine succinylation during human erythroid differentiation.

View Article and Find Full Text PDF

Peptostreptococcus anaerobius plays an important role in the development of colorectal cancer, and previous studies by our group have demonstrated that Peptostreptococcus anaerobius promotes resistance to 5-Fu chemotherapy in animal models of colorectal cancer. In this study, the effects of Peptostreptococcus anaerobius on chemotherapy resistance in colorectal cancer and its possible mechanism of action were investigated from the clinical point of view. Patients were selected according to exclusion and inclusion criteria and divided into sensitive and chemotherapy groups (n = 20/group).

View Article and Find Full Text PDF

In this study, the N-Acetyl-l-Cysteine (NAC)-capped gold and silver bimetallic nanoclusters (NAC@Au-Ag NCs) was synthesized by reflux method. Due to the silver effect, the NAC@Au-Ag NCs exhibited strong photoluminescence at far-red/near-infrared regions and better catalytic performance than Au or Ag NCs. Upon addition of HS, the Au-Ag NCs exhibited obvious fluorescence quench and color changes through the generation of metal sulfides and a static quenching process.

View Article and Find Full Text PDF

Iterative Bleaching Extends multipleXity (IBEX) is a versatile method for highly multiplexed imaging of diverse tissues. Based on open science principles, we created the IBEX Knowledge-Base, a resource for reagents, protocols and more, to empower innovation.

View Article and Find Full Text PDF

Gangliocytic paraganglioma (GP) is a rare neuroendocrine tumor primarily found in the duodenum, most commonly in the second and third sections of the duodenum. Diagnosis of GP is based on its distinctive histopathological characteristics, which include three types of tumor cells in varying proportions: i) Epithelioid, ii) spindle-like and iii) ganglion-shaped cells. The distribution of the three tumor cell components varies from case to case and a patient may be easily misdiagnosed if one of the components is predominant.

View Article and Find Full Text PDF
Article Synopsis
  • * The researchers found that when TMEM56 is lost, it disrupts the production of haem (a component crucial for hemoglobin) and negatively affects the differentiation of erythroid cells derived from umbilical cord blood stem cells.
  • * In mouse models, deleting the TMEM56 gene in the erythroid lineage led to problems with erythroid progenitor cells in the bone marrow, highlighting its essential function in maintaining healthy red blood cell production and suggesting it might be a new target for treating blood disorders.
View Article and Find Full Text PDF

Bacterial virulence plays an important role in infection. Antibacterial virulence factors are effective for preventing crop bacterial diseases. Resin acid copper salt as an effective inhibitor exhibited excellent anti- pv.

View Article and Find Full Text PDF

Cereblon (CRBN) has been successfully co-opted to affect the targeted degradation of "undruggable" proteins with immunomodulatory imide drugs (IMiDs). IMiDs act as molecule glues that facilitate ternary complex formation between CRBN and a target protein, leading to ubiquitination and proteasomal degradation. Subtle structural modifications often cause profound and sometimes unpredictable changes in the degradation selectivity.

View Article and Find Full Text PDF

Soil organic carbon (SOC) plays a vital role in maintaining or enhancing soil fertility and quality of paddy field, but there is still limited information about how SOC mineralization responds to different tillage managements under the double-cropping rice (Oryza sativa L.) system in southern of China. Therefore, this study was designed to explore the changes in SOC content, soil enzyme activities (invertase, cellulose and urease), SOC mineralization at 0-10 cm and 10-20 cm layers and its relationship with 7-years tillage management under the double-cropping rice system of southern China.

View Article and Find Full Text PDF

User-friendly in-field sensing protocol is crucial for the effective tracing of intended analytes under less-developed countries or resources-limited environments. Nevertheless, existing sensing strategies require professional technicians and expensive laboratory-based instrumentations, which are not capable for point-of-care on-site analyses. To address this issue, artificial intelligence handheld sensor has been designed for direct reading of Ni and EDTA in food samples.

View Article and Find Full Text PDF
Article Synopsis
  • Lymphoma is a type of cancer that originates in the lymphatic system and can affect various tissues and organs, showing significant diversity in treatment response and prognosis based on its type and stage.
  • Early detection of lymphoma is crucial for improving patient outcomes, leading to a study that compared the diagnostic effectiveness of CT, ultrasound, and PET-CT in identifying lymphoma.
  • The study found that PET-CT and its combination with other imaging techniques had significantly higher sensitivity and specificity for diagnosing lymphoma compared to CT and ultrasound alone, indicating that PET-CT is a valuable tool for early detection.
View Article and Find Full Text PDF

Background: Identifying possible influencing factors is crucial for the depression symptoms of women experiencing infertility. This study aims to explore the association between polyunsaturated fatty acids (PUFAs) and the odds of depression symptoms in women experiencing infertility.

Methods: This is a cross-sectional study based on the National Health and Nutrition Examination Survey (NHANES).

View Article and Find Full Text PDF

Soil extracellular enzyme plays a vital role in changing soil nitrogen (N) mineralization of rice field. However, the effects of soil extracellular enzyme activities (EEA) and microbial community composition response to N mineralization of rice field under short-term tillage treatment needed to be further explored. In this study, we investigated the impact of short-term (8-year) tillage practices on rhizosphere soil N transformation rate, soil enzyme activities, soil microbial community structure, and the N mineralization function gene abundances in double-cropping rice field in southern China.

View Article and Find Full Text PDF

Inherited non-hemolytic anemia is a group of rare bone marrow disorders characterized by erythroid defects. Although concerted efforts have been made to explore the underlying pathogenetic mechanisms of these diseases, the understanding of the causative mutations are still incomplete. Here we identify in a diseased pedigree that a gain-of-function mutation in toll-like receptor 8 (TLR8) is implicated in inherited non-hemolytic anemia.

View Article and Find Full Text PDF

Erythroid cells, the most prevalent cell type in blood, are one of the earliest products and permeate through the entire process of hematopoietic development in the human body, the oxygen-transporting function of which is crucial for maintaining overall health and life support. Previous investigations into erythrocyte differentiation and development have primarily focused on population-level analyses, lacking the single-cell perspective essential for comprehending the intricate pathways of erythroid maturation, differentiation, and the encompassing cellular heterogeneity. The continuous optimization of single-cell transcriptome sequencing technology, or single-cell RNA sequencing (scRNA-seq), provides a powerful tool for life sciences research, which has a particular superiority in the identification of unprecedented cell subgroups, the analyzing of cellular heterogeneity, and the transcriptomic characteristics of individual cells.

View Article and Find Full Text PDF

Continuous rotation of a fragile, photosensitive microrod in a safe, flexible way remains challenging in spite of its importance to microelectro-mechanical systems. We propose a photovoltaic strategy to continuously rotate a fragile, fluorescent microrod on a LiNbO/Fe (LN/Fe) substrate using a continuous wave visible (473 nm) laser beam with an ultralow power (few tens of μW) and a simple structure (Gaussian profile). This strategy does not require the laser spot to cover the entire microrod nor does it result in a sharp temperature rise on the microrod.

View Article and Find Full Text PDF

Osteoarthritis (OA) is increasing worldwide, and previous work found that OA increases systemic cartilage oligomeric matrix protein (COMP), which has also been implicated in prostate cancer (PCa). As such, we sought to investigate whether OA augments PCa progression. Cellular proliferation and migration of RM1 murine PCa cells treated with interleukin (IL)-1α, COMP, IL-1α + COMP, or conditioned media from cartilage explants treated with IL-1α (representing OA media) and with inhibitors of COMP were assessed.

View Article and Find Full Text PDF