Sonography on injury of the medial patellofemoral ligament after acute traumatic lateral patellar dislocation: Injury patterns and correlation analysis with injury of articular cartilage of the inferomedial patella.

Objective: The purpose of this study was to investigate the accuracy of high-frequency ultrasonography in the diagnosis of injuries of medial patellofemoral ligaments (MPFLs), analyse the characteristics of MPFL injury and correlations between injury of the MPFL and articular cartilage of the inferomedial patella in patients with acute traumatic lateral patellar dislocation.

Methods: High-frequency sonographic images of 49 patients with acute traumatic lateral patellar dislocations treated surgically were reviewed. The χ(2) tests were performed for statistical analysis.

miR-141 Regulates colonic leukocytic trafficking by targeting CXCL12β during murine colitis and human Crohn's disease.

Objective: Emerging evidence suggests that microRNA (miRNA)-mediated gene regulation influences a variety of autoimmune disease processes, including Crohn's disease (CD), but the biological function of miRNAs in CD remains unclear. We examine miRNA level in colon tissues and study the potential functions of miRNAs that regulate pathological genes during the inflammation process.

Design: miRNA levels were assayed in the inflamed colon of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced and IL-10 knockout (KO) chronic colitis mice and CD patients by microarray or qRT-PCR.

Application of RIFLE criteria in patients with multiple myeloma with acute kidney injury: a 15-year retrospective, single center, cohort study.

Abstract Although there have been numerous studies of patients with multiple myeloma (MM) and acute kidney injury (AKI), the results from these studies have varied greatly because of inconsistent definitions of AKI. The RIFLE criteria, which were designed to standardize the staging of AKI, have been extensively validated worldwide, but rarely in patients with MM. We retrospectively analyzed the natural history of 78 patients with MM and AKI between July 1995 and December 2010.

The pilot study of dual-energy CT gemstone spectral imaging on the image quality of hand tendons.

Purpose: The aim of this study was to evaluate the image quality of hand tendons using dual-energy computed tomographic gemstone spectral imaging (DECT GSI) compared with conventional CT images.

Materials And Methods: Forty patients scanned with GSI mode on DECT were enrolled. The 65-keV optimal contrast-to-noise ratio for viewing hand tendons was selected.

Evaluation of anemia and serum iPTH, calcium, and phosphorus in patients with primary glomerulonephritis.

Glomerulonephritis (GN) remains a major cause of morbidity and mortality in chronic kidney disease (CKD). Our study aimed to investigate the prevalence of anemia, abnormal serum intact parathyroid hormone (iPTH), calcium, and phosphorus in a Chinese patient population with primary GN. Medical histories and laboratory test results were collected from 2,924 patients with primary GN hospitalized in Ruijin Hospital of Shanghai between January 2003 and August 2009.

Acquired hemophilia a: retrospective analysis of 49 cases from a single Chinese hemophilia center.

Acquired hemophilia A (AHA) is a rare bleeding disorder caused by the autoantibody directed against factor VIII in patients without previous history of a bleeding disorder. We retrospectively analyzed the characteristics and outcomes of 49 patients with AHA diagnosed in our center from February 1994 to October 2012. Twenty-four patients with acute bleeding episodes were treated with prothrombin complex concentrate (PCC) at a relative low dose of 30 to -50 U/kg/d and achieved good outcomes without any adverse reaction.

Improved regenerative myogenesis and muscular dystrophy in mice lacking Mkp5.

Duchenne muscular dystrophy (DMD) is a degenerative skeletal muscle disease caused by mutations in dystrophin. The degree of functional deterioration in muscle stem cells determines the severity of DMD. The mitogen-activated protein kinases (MAPKs), which are inactivated by MAPK phosphatases (MKPs), represent a central signaling node in the regulation of muscle stem cell function.

Biochemical properties of a novel thermostable and highly xylose-tolerant β-xylosidase/α-arabinosidase from Thermotoga thermarum.

Background: β-Xylosidase is an important constituent of the hemicellulase system and it plays an important role in hydrolyzing xylooligosaccharides to xylose. Xylose, a useful monose, has been utilized in a wide range of applications such as food, light, chemical as well as energy industry. Therefore, the xylose-tolerant β-xylosidase with high specific activity for bioconversion of xylooligosaccharides has a great potential in the fields as above.

A novel highly thermostable xylanase stimulated by Ca2+ from Thermotoga thermarum: cloning, expression and characterization.

Background: Xylanase is an important component of hemicellulase enzyme system. Since it plays an important role in the hydrolysis of hemicellulose into xylooligosaccharides (XOs), high thermostable xylanase has been the focus of much recent attention as powerful enzyme as well as in the field of biomass utilization.

Results: A xylanase gene (xyn10A) with 3,474 bp was cloned from the extremely thermophilic bacterium Thermotoga thermarum that encodes a protein containing 1,158 amino acid residues.

Abnormal glucose metabolism and insulin sensitivity in Chinese patients with Gitelman syndrome.

Background: Gitelman syndrome (GS) is an autosomal recessive disease of renal tubulopathy, primarily characterized by hypokalemic metabolic alkalosis with significant hypomagnesemia, low urinary calcium, secondary aldosteronism and normal blood pressure. Both hypokalemia and hypomagnesemia were reported to cause impaired glucose tolerance and/or insulin resistance, but it is unclear whether impaired glucose tolerance and/or insulin resistance are common in GS patients.

Methods: An oral glucose tolerance test was performed among 16 GS patients and 12 healthy adults as control subjects.

Particle-induced osteolysis mediated by endoplasmic reticulum stress in prosthesis loosening.

We hypothesized that endoplasmic reticulum (ER) stress in macrophages induced by wear particles was one of the reasons for particle-induced osteolysis (PIO) in total hip arthroplasty (THA) failure. In the present study, the expression of ER stress markers was examined by Western blot in macrophages treated with particles from materials used in prosthetics, specimens from PIO animal models and patients suffering from aseptic loosening. To address whether ER stress triggers these inflammatory responses, the effect of an ER stress blocker on the expression of inflammatory cytokines in particle-treated macrophages and PIO animal models was tested.

Antibiotics in the surface water of the Yangtze Estuary: occurrence, distribution and risk assessment.

The occurrence and distribution of five groups of antibiotics were investigated in the surface water of Yangtze Estuary over four seasons. Of the 20 antibiotics, only sulfamerazine was not detected at all sampling sites, indicating widespread occurrence of antibiotic residues in the study area. Detection frequencies and concentrations of antibiotics were generally higher in January, indicating that low flow conditions and low temperature might enhance the persistence of antibiotics in water.

Predicting progression of IgA nephropathy: new clinical progression risk score.

IgA nephropathy (IgAN) is a common cause of end-stage renal disease (ESRD) in Asia. In this study, based on a large cohort of Chinese patients with IgAN, we aim to identify independent predictive factors associated with disease progression to ESRD. We collected retrospective clinical data and renal outcomes on 619 biopsy-diagnosed IgAN patients with a mean follow-up time of 41.

Diversity and specificity of the mitogen-activated protein kinase phosphatase-1 functions.

The balance of protein phosphorylation is achieved through the actions of a family of protein serine/threonine kinases called the mitogen-activated protein kinases (MAPKs). The propagation of MAPK signals is attenuated through the actions of the MAPK phosphatases (MKPs). The MKPs specifically inactivate the MAPKs by direct dephosphorylation.

Thermoanaerobacterium thermosaccharolyticum β-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose.

Background: β-Glucosidase is an important component of the cellulase enzyme system. It does not only participate in cellulose degradation, it also plays an important role in hydrolyzing cellulose to fermentable glucose by relieving the inhibition of exoglucanase and endoglucanase from cellobiose. Therefore, the glucose-tolerant β-glucosidase with high specific activity for cellobiose might be a potent candidate for industrial applications.

[Analysis of arterial spin labeling in 33 patients with hypoxic ischemic encephalopathy].

Objectives: To evaluate the diagnostic value of arterial spin labeling (ASL) technology in newborns with hypoxic ischemic encephalopathy (HIE).

Method: Seven full-term newborn infants without any history of asphyxia and other nervous system diseases were selected as the control and 33 full-term newborn infants were assigned into HIE group. The patients in HIE group were further divided into three subgroups (19 cases of mild, 6 cases of moderate and 8 cases of severe HIE) based on their clinical diagnosis.

[Radiological research of maxillary sinus development of children and adolescent without sinusitis in Kunming of Yunnan].

Objective: To investigate the maxillary sinus development and provide data for sinus surgery on children.

Method: Two thousand two hundred and thirty-eight children were randomly selected among those who underwent skull and nasal sinus CT scanning because of certain symptoms and signs. Patients were divided into eighteen age cohorts based on their age at the time of the scan.

Magnesium sulfate as an oral contrast medium in magnetic resonance imaging of the small intestine.

Background: To explore the use of magnesium sulfate (MgSO4) as an oral contrast medium (CM) in MRI of the small intestine.

Methods: By comparing MgSO4 SNRs at different concentrations, we determined that 2.5% MgSO4 is the ideal concentration for small bowel MRI.

Application of RIFLE criteria in Chinese patients with ANCA-associated renal vasculitis.

Objectives: Antineutrophil cytoplasmic autoantibody (ANCA) associated small vessel vasculitis (AASV) constitutes a group of life-threatening diseases and renal involvement is its most severe and common manifestation. Acute kidney injury (AKI) is common in patients with AASV but the value of RIFLE criteria is still unclear in those patients.

Methods: We performed a retrospective study on patients with AASV in Shanghai Ruijin Hospital from 1997 to 2008.

Analyzing Chinese patients with post-operative acute kidney injury.

Background: To investigate clinical characteristics and risk factors of Chinese patients with post-operative acute kidney injury (PO-AKI).

Methods: Patients with PO-AKI in Ruijin Hospital from December 1997 to December 2005 were retrospectively studied.

Results: Patients' mean age was 62.

Chronic kidney disease is prevalent in Chinese patients admitted with verified cerebrovascular lesions and predicts short-term prognosis.

Objective: Recent studies have identified chronic kidney disease (CKD) as an important risk factor for vasculopathies. While the link between CKD and cardiovascular events is well established, the link with cerebrovascular lesions (CVL) has been less well described.

Methods: We studied the prevalence and prognostic importance of CKD in 1014 incident Chinese patients admitted with verified computerized tomography (CT) or magnetic resonance imaging (MRI) CVL (within the last 7 days).

[Comparison of the nasal sinus development of children with or without sinusitis in Yunnan province].

Objective: To investigate the nasal sinus development and discuss the relation between sinusitis and nasal development.

Method: One thousand seven hundred and five healthy children and 1424 children with sinusitis were selected randomly. All children underwent naso sinus computer tomography.

[Research of the nasal sinusitis incidence of children in Kunming of Yunnan Province].

Objective: In order to make a basis for the treatment of nasal sinuses of children and the correlated disease, we investigate the incidence rate of nasal sinusitis of children and explore the correlation between nasal sinusitis of children and CT in Kunming.

Method: Two thousand one hundred and fourteen healthy children and 1535 children with sinusitis were selected randomly. Form whom were undergone nasal sinus computer tomography for different medical reasons.

MAP kinase phosphatase-1 deficiency impairs skeletal muscle regeneration and exacerbates muscular dystrophy.

In skeletal muscle, the mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1) is a critical negative regulator of the MAPKs. Since the MAPKs have been reported to be both positive and negative for myogenesis, the physiological role of MKP-1 in skeletal muscle repair and regeneration has remained unclear. Here, we show that MKP-1 plays an essential role in adult regenerative myogenesis.