Casein kinase 1 (CK1) promotes the proliferation and metastasis of glioma cells via the phosphatidylinositol 3 kinase-matrix metalloproteinase 2 (AKT-MMP2) pathway.

Background: Glioma is a type of tumor that usually occurs in the adult central nervous system. Protein kinases have become important targets for oncotherapy since they are closely correlated with signal transduction. The role of the casein kinase 1 (CK1) gene in glioma remains to be fully elucidated.

Methylation and serum response factor mediated in the regulation of gene ARRDC3 in breast cancer.

Breast cancer poses a serious threat to women's life and health and many factors contribute to breast cancer including gene mutation and epigenetics. Gene ARRDC3 was usually repressed in breast cancer and methylation in promoter was reported to be involved in gene ARRDC3 expression regulation. To this end, the methylation status for gene ARRDC3 promoter was assayed by the Massarray quantitative method.

Repair of spinal cord injury by chitosan scaffold with glioma ECM and SB216763 implantation in adult rats.

The loss of spinal cord tissue and the cavity formation are major obstacles to the repair of spinal cord injury (SCI). In the study, the scaffold of chitosan+ECM+SB216763 was fabricated and used for the repair of injured spinal cord injury. First, the biocompatibility of the scaffold was analyzed and results showed that the scaffold had a good compatibility with the neural stem cells.

Promoter methylation assay of SASH1 gene in breast cancer.

Purpose: To analyze the relationship between the expression of SASH1 and its methylation level of SASH1 gene promoter in human breast cancer.

Methods: Expression levels of SASH1 were examined in breast cancer tissues and adjacent normal tissues with immunohistochemistry and with real time PCR (RT-PCR) methylation analysis was performed with MassArray.

Results: Immunohistochemistry showed that SASH1 expression was strongly reduced in breast cancer compared with adjacent normal tissues.

The association between GAD1 gene polymorphisms and cerebral palsy in Chinese infants.

Studies suggest that GAD1 gene was a functional candidate susceptibility gene for cerebral palsy (CP). In order to investigate the contribution of GAD1 gene to the etiology of CP in Chinese infants, we carried out a case-control association study between GAD1 gene and CP. In this study, 374 health controls and 392 infants with CP were recruited.

Association of Interleukin 6 gene polymorphisms with genetic susceptibilities to spastic tetraplegia in males: a case-control study.

Background: Cerebral palsy (CP) is a group of non-progressive motor impairment and permanent disorders causing limitation of activity and abnormal posture. It may be caused by infection (such as chorioamnionitis), asphyxia or multiple genetic factors. The Interleukin 6 gene (IL6) was suggested to be involved in the susceptibilities to CP risk as a kind of proinflammatory cytokine.

The role of the TOB1 gene in growth suppression of hepatocellular carcinoma.

The TOB1 gene, mapped on 17q21, is a member of the BTG/Tob family. In breast cancer it has been identified as a candidate tumor suppressor gene. However, whether TOB1 is a bona fide tumor suppressor and downregulated in hepatocellular carcinoma (HCC) remains unclear.

Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro.

The SAM and SH3 domain containing 1 (SASH1) gene was originally identified as a potential tumor suppressor gene in breast cancer, mapped on chromosome 6q24.3. The expression of SASH1 plays a prognostic role in human colon cancer.

Establishment and characterization of two cell lines derived from primary cultures of Gekko japonicus cerebral cortex.

Adult Gekko japonicus is one of those vertebrates that are able to regenerate their missing or amputated tail. The most interesting feature of this animal lies in the ability of its spinal cord to regrow a functional tail. A fundamental question is whether the neuroglial cells play a different role compared with high vertebrates.

Effect of chitooligosaccharide on neuronal differentiation of PC-12 cells.

Chitosan is now being widely used biomaterial in the tissue engineering field, and has great potential as a candidate material for preparing nerve guidance conduits due to its various favorable properties, especially that of good nerve cell affinity. Chitosan can be degraded in vivo into chitooligosaccharide. We have investigated the in vitro effects of chitooligosaccharide on neuronal differentiation of PC-12 cells to see what effects chitooligosaccharide have on certain functions in the regenerating neurons.

Tpc1808 increases expression of NF-H in PC12 cells.

Tpc1808 is a candidate chemotropic factor induced by nerve injury, and we report here the role of rat Tpc1808 in the expression of high-molecular-weight neurofilament (NF-H) in PC12 cells. A full-length Tpc1808 cDNA encoding the 275-amino-acid protein was constructed into pcDNA-HA and transfected into PC12 cells. The increased expression of NF-H was detectable in PC12-Tpc1808 clones by semi-qRT-PCR, real-time PCR, Western blot, and immunofluorescence.