[Experience in the use of extracorporeal correction of the immune system in the treatment of patients with LOR cancer].

Correction of the immune status is one of the burning problems of current otorhinolaryngology. Recent developments in immunology allow interference at the cellular level. However, otorhinolaryngological practice is still based on oral, parenteral or local use of ineffective drugs.

[A comparative analysis of the effect of clofelin on gastric secretory function in man and dog].

It is established that clofelin suppresses the human and dog insulin gastric secretion as well as human basal and histamine secretion. Clofelin has no effect on the secretory function of the dog stomach, stimulated by pentagastrin, carbachol histamine. It is supposed that clofelin-induced suppression of the human histamine gastric secretion takes place due to the inhibition of basal secretory component being a part of the common secretory effect on histamine.

[Immunochemical identification and physico-chemical characteristics of specific alpha 2 globulin of human granulocytes].

The antigen which has alpha 2-globulin mobility was identified immunochemically in leukolysate and pus extract. This antigen was localized in polymorphonuclear leukocytes by the immunofluorescence technique in the blood of healthy donors. The alpha 2-globulin of granulocytes (alpha 2GG) is not identical to lactoferrin, lysozyme, granulocyte elastase, fibronectin, fetal hemoglobin, amyloid P-component of the serum.

[Quantitative immunoenzyme determination of lactoferrin in the serum and plasma].

A procedure for quantitative estimation of lactoferrin is developed using the "sandwich" type of immunoenzymatic assay. Preparation of lactoferrin was purified from women colostrum by means of ion exchange chromatography; the sorbent (a ligand-containing complex of lactoferrin) was synthesized; the antibody conjugate against lactoferrin and peroxidase from horseradish was prepared; optimal conditions of the immunoenzymatic assay were selected. Concentration of lactoferrin in donor blood serum or plasma constituted 0.

[Dynamics of granulocyte proteins after lymphocytapheresis in patients with rheumatoid arthritis].

The authors studied concentrations of granulocytic proteins, lactoferrin, leukocytic thermostable alpha-glycoprotein in the blood plasma of patients with rheumatoid arthritis. High concentrations of the above proteins were observed in RA patients.

[Levels of the C 4 component of the complement, lactoferrin and leukocytic thermostabile alpha glycoprotein during the treatment of patients with systemic rheumatic diseases].

The authors studied the dynamics of C4-component of complement (C4), lactoferrin (LF), leukocytic thermostable alpha-glycoprotein (LT alpha G) in the blood serum of 54 patients with rheumatoid arthritis, 32 patients with systemic lupus erythematosus, 8 patients with systemic vasculitis under the effect of hemosorption (HS), enterosorption (ES) and also immunosuppressants (IS). In severe forms of systemic rheumatic diseases the blood serum exhibits a decreased content of C4 but an elevated level of LF and LT alpha G. It has been established that HS is more active than ES or IS, and it influences the mechanisms of humoral immunity.

[Interaction of block-polyurethane containing lactose linkages and immobilized beta-galactosidase with body tissues].

Biodegradation of the polymeric composition containing immobilized beta-galactosidase after implantation and its effect on the surrounding tissues are examined. Immunogenicity of the composition is studied by the method of the immunoenzymic analysis. Activation of the immune system is shown to occur in the process of the composition degradation.

[Leukocytic thermostable alpha-glycoprotein and lactoferrin from granulocyte cytoplasm (immunocytochemical study)].

The immunofluorescence techniques with monospecific antibodies to lactoferrin (LF) and leukocytic thermostable alpha-glycoprotein (LT alpha-G) has demonstrated that both the proteins are specific components of the peripheral blood granulocytes of normal subjects. LF and LT alpha-G are detectable in the cytoplasmic granules of polynuclear leukocytes but not on the external surface of these cells' membranes. Differences between the direct and indirect immunofluorescence techniques, used for the detection of the granulocytic LF and LT alpha-G are discussed.

[Correction (in vitro) of proteinase inhibitor balance in the blood of patients after cardiosurgical interventions].

Proteinase-inhibitor balance in human plasma before and after artificial blood circulation was examined. It was shown that trypsinlike and chemotrypsinlike proteinases activities after this procedure were 1.6 and 2.

[Inhibition of human granulocyte elastase by antibodies to leukocyte thermostable alpha-glycoprotein].

The activity of granulocyte elastase (GE) was discovered in the preparations of leukocyte thermostable alpha-glycoprotein (LTG) isolated from pus by means of ion-exchange chromatography. The activity of GE was determined according to MeoSuc(Ala)2ProValpNa hydrolysis. The antibodies against LTG were isolated from monospecific antisera.

[Determination of the elastase activity of the leukocyte thermostable alpha-glycoprotein from human blood plasma].

The possibility of isolation of specific human granulocyte antigen--leukocyte thermostable alpha-glycoprotein (LTG) from plasma was shown. The isolation of preparations containing LTG was carried out on CNBr-activated sepharose with immobilized soluble fraction of pus. Using immunochemical analysis with standard antisera, the absence of plasma and leukocyte proteins and the presence of LTG in the preparations obtained were demonstrated.

[Immunochemical determination of the heterogeneity of leukocyte thermostable alpha-glycoprotein].

A comparative immunochemical and physico-chemical study of leukocyte thermostable alpha-glycoprotein (LTG) has been performed in pus extract, hemolysate, leukolysate and blood plasma. Immunochemically determined LTG is heterogeneous in respect of molecular charge, sialic acid content and temperature stability. LTG in the blood plasma has a form of alpha-globulin, while in hemolysate it is a beta-globulin, with both forms present in pus.

[Thermostable leukocyte alpha-glycoprotein: immunochemical study and enzyme activity research].

Using immunochemical analysis with standard antisera, leukocyte thermostable alpha-glycoprotein (LT alpha G) was shown to be distinct from lactoferrin, lysozyme, and fibronectin. The determination of peroxidase and nonspecific elastase in immune precipitates of LT alpha G gave negative results. Affinity sorption of LT alpha G onto the pus protein component was revealed.

[The state of the central glucoreceptor mechanism of the regulation of gastric secretion in patients with duodenal ulcer with different variants of its course].

The time course of the levels of glycemia, gastric secretion of acid and pepsin, and indices of variation pulsometry in response to intravenous injection of glucose (0.66 g/kg) was studied in 42 patients with duodenal ulcer and in 11 healthy persons. It was established that in duodenal ulcer the reactivity of the central glucoreceptor mechanism regulating acid and pepsin secretion, was considerably elevated, especially in patients with complicated forms of disease and gastric hypersecretion.

[Use of the hyperventilation test in patients with various forms of angina].

The pulmonary hyperventilation (HV) test was carried out in 31 coronary patients with stable angina and 11 patients with spontaneous (angiospastic) angina. The test results were compared to those obtained with bicycle ergometry (BE), 24-hour ECG monitoring and selective angiography. The HV test was positive in 7 of 24 patients with positive BE results.

[Comparative immunochemical and immunocytochemical study of 3 soluble granulocytic proteins in blood cells and serum].

Using immunofluorescence, it has been shown that leukocyte thermostable alpha-glycoprotein (LTAG) and leukocyte beta-globulin (LBG) are, like lactoferrin, components of polynuclear leukocytes. These proteins were found in the perinuclear zone, cytoplasm membrane and extracellularly. Serum LTAG concentration increases in immune inflammatory diseases.

[Dynamics of coronary artery lumina during tests with cold and with intracoronary and sublingual administration of nitroglycerin in patients with different clinical forms of stenocardia].

Cold tests and those using intracoronary or sublingual nitroglycerin administration were applied during coronarography to 50 coronary patients with varying clinical manifestations of angina pectoris. The quantitative assessment of angiographic patterns showed the cold and nitroglycerin tests to change significantly the extent of stenosis in patients with spontaneous angina (by +13.3 and -30.

[Complex immunochemical study of plasma proteins and soluble antigens of leukocytes in patients with ischemic heart disease during hemoperfusion therapy].

An immunochemical analysis of 16 individual plasma proteins and 8 leukocyte soluble antigens (LSA) was performed over time in 11 coronary patients after hemosorption. The concentration of plasma lipoproteins gradually returned to normal within 2-3 weeks. Leukocyte dysfunction in the patients manifested itself in change of the specific LSA concentration in hemolysates as compared to that in donors.