Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study.

Background: Due to non-vascularized and aneural structure, articular cartilage has limited self-repairing capacity. The aim of this study was to investigate the revitalization of inflammatory injured articular cartilage matrices by human nasal chondrocytes (hNC).

Materials And Methods: Cartilage matrix was prepared by devitalization of articular cartilage samples obtained intraoperatively from an adult patient undergoing knee joint replacement.

Biological Efficacy of Ionizing Radiation Sources on 3D Organotypic Tissue Slices Assessed by Fluorescence Microscopy.

Objective: Traditional cell-based radiobiological methods are inadequate for assessing the toxicity of ionizing radiation exposure in relation to the microstructure of the extracellular matrix. Organotypic tissue slices preserve the spatial organization observed in vivo, making the tissue easily accessible for visualization and staining. This study aims to explore the use of fluorescence microscopy of physiologically compatible 3D tissue cultures to assess the effects of ionizing radiation.

Refinement of Animal Experiments: Replacing Traumatic Methods of Laboratory Animal Marking with Non-Invasive Alternatives.

Reliable methods for identifying rodents play an important role in ensuring the success of preclinical studies. However, animal identification remains a trivial laboratory routine that is not often discussed, despite the fact that more than 6 million rodents are used in animal studies each year. Currently, there are extensive regulations in place to ensure adequate anesthesia and to reduce animal suffering during experiments.

Relationship of Structural and Tissue Components of Full-Layer Skin Wound and Mathematical Modeling of the Healing Process.

We studied the relationship between structural components of the wound (scab, leukocyte shaft, newly formed epithelium, and granulation tissue) and tissue components (epithelial and connective tissue) during spontaneous healing of skin wounds in rats. The regenerate structures were evaluated by microscopic morphometry. The number of vessels in 10 fields of view was calculated (at ×600), the dimensions of newly formed regenerate structures were determined: height of the scab, leukocyte shaft, granulation tissue, the border zone of the epithelium, and the length of the epithelial wedge.

[The sociomedical portrait of girls studying at a secondary special educational establishment].

The purpose of this investigation was to study a social portrait in 15-17-year-old girls studying at secondary specialized educational establishments and the characteristics of their families. The urban girls have better living conditions than the rural ones. The income of the families of first- and second-year students from other towns was significantly more frequently lower (63.

[Cytological evaluation of laser therapy influence upon skin reparation].

Evidence on the studying of the effectiveness the low intensive laser radiation influence as complex treatment of patient with maxillofacial phlegmons with using of the cytological method were presented. Results of the laser therapeutist were in acceleration of the inflammation stage. The neutrophil granulocytes and macrophages settled down in wound earlier then in the control.

[Consumption of tea among population on the far north of Russia].

Aim of present study was the getting data on tea and coffee consumption among population on the Far North by 24-hour recall of dietary intake. About 84% of population have consumed the black tea. Mean daily black and green tea intake among all population were 335 ml and 49 ml, correspondingly.

[Gastroduodenal mucosal morphological changes in tuberculosis-infected children].

Three groups of children aged 4 to 14 years who complained of having dyspepsia were examined. Group 1 included 31 children without tuberculous infection; Group 2 comprised 30 newly tuberculosis-infected children; Group 3 consisted of 35 children who had an over one-year history of the infection. Esophagoduodenoscopy and biopsy indicated that the incidence of Helicobacter infection and gastritis was higher in children with tuberculous infection than in those without it and it was directly related to the duration of infection.