A comparison of wet-dry swabbing and excision sampling methods for microbiological testing of bovine, porcine, and ovine carcasses at red meat slaughterhouses.

A comparison of wet-dry swabbing and surface tissue excision of carcasses by coring was undertaken. Samples from 1,352 bovine, 188 ovine, and 176 porcine carcasses were collected from 70 separate visits to commercial slaughterhouses operating under normal conditions. The mean total aerobic viable bacterial counts (TVCs) for all species sampled by excision was 5.

Fate of pathogens present in livestock wastes spread onto fescue plots.

Fecal wastes from a variety of farmed livestock were inoculated with livestock isolates of Escherichia coli O157, Listeria monocytogenes, Salmonella, Campylobacter jejuni, and Cryptosporidium parvum oocysts at levels representative of the levels found in naturally contaminated wastes. The wastes were subsequently spread onto a grass pasture, and the decline of each of the zoonotic agents was monitored over time. There were no significant differences among the decimal reduction times for the bacterial pathogens.

Antilisterial Effects of a Sorbate-Nisin Combination In Vitro and on Packaged Beef at Refrigeration Temperature.

The antilisterial effects of a sorbate-nisin combination were assessed in vitro and on beef at refrigeration temperature. Three hemolytic pathogenic strains of Listeria monocytogenes , reference strain NCTC 7973, food strain L70, and clinical strain L94, were stored at 4°C in phosphate-buffered saline, pH 5.5, containing a combination of sorbate (0.

Inhibition of Listeria monocytogenes on Normal Ultímate pH Beef (pH 5.3-5.5) at Abusive Storage Temperatures by Saturated Carbon Dioxide Controlled Atmosphere Packaging.

Beef striploin steaks (each weighing between 60 and 100 g) of normal ultimate pH (average 5.45) were inoculated with a mixture of two food isolates of Listeria monocytogenes , packaged under saturated carbon dioxide controlled atmosphere or vacuum, and stored at either 5 or 10°C. Saturated carbon dioxide packaging but not vacuum packaging inhibited growth of L.