Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during shoot regeneration of callus: Implications for bioprocess routes to plant-made biopharmaceuticals.

Transplastomic plants are capable of high-yield production of recombinant biopharmaceutical proteins. Plant tissue culture combines advantages of agricultural cultivation with the bioprocess consistency associated with suspension culture. Overexpression of recombinant proteins through regeneration of transplastomic shoots from callus tissue in RITA temporary immersion bioreactors has been previously demonstrated.