In vitro evaluation of the effect of the electronic cigarette aerosol, Cannabis smoke, and conventional cigarette smoke on the properties of gingival fibroblasts/gingival mesenchymal stem cells.

Objective: The current study aimed to evaluate the effect of electronic cigarette (EC) aerosol, Cannabis, and conventional cigarettes smoke on gingival fibroblast/gingival mesenchymal stem cells' (GF/G-MSCs) of never smokers.

Material And Methods: Human GF/G-MSCs (n = 32) were isolated and characterized using light microscopy, flow cytometry, and multilineage differentiation ability. Following the application of aerosol/smoke extracts, GF/G-MSCs were evaluated for cellular proliferation; colony-forming units (CFU-F) ability; cellular viability (using the MTT assay); mitochondrial depolarization using JC-1 dye; and genes' expression of ATM, p21, Oct4, and Nanog.

Influence of Small, Midi, Medium and Large Fields of View on Accuracy of Linear Measurements in CBCT Imaging: Diagnostic Accuracy Study.

Aim: This study aimed to assess the effect of changing the field of view on the dimensional accuracy of CBCT imaging.

Methods: The implant-bone models were randomly numbered from 1 to 13 by the principal researcher, and then on each model at the incisors region three positions were selected and marked on the model with a permanent blue marker. Then at each marked position three radio-opaque 'RO' markers "gutta-percha pieces" were glued on the model surfaces as following; two pieces on the facial surface one occlusally (at the alveolar crest) and one apically (at the inferior border of the model) both were on the same vertical line and perpendicular to the horizontal plane, while the third one was placed on the lingual surface opposing the occlusally placed buccal piece.

Characteristics of Human Natal Stem Cells Cultured in Allogeneic Medium.

Recently, human natal dental pulp stem cells (hNDP-SCs) have been characterized in vitro and it has been shown that they satisfy criteria defining human mesenchymal stromal cells (MSCs), as proposed by the International Society for Cellular Therapy. However, these results were reached in the presence of xenogeneic expansion medium, which has the potential to alter the cells' functional capacity. To determine the validity of the previously reported hNDP-SCs characteristics for human cell therapy, we have cultured hNDP-SCs in allogeneic expansion medium.

Effect of Laser Therapy on the Osseointegration of Immediately Loaded Dental Implants in Patients under Vitamin C, Omega-3 and Calcium Therapy.

Background: The use of laser therapy in the biostimulation of bone repair has been growing steadily.

Aim: This study aimed to evaluate the radio-densitometric effect of low-intensity laser therapy on the osseointegration of immediately loaded dental implants in patients under vitamin C, omega-3 and calcium therapy.

Patients And Methods: A single implant was placed in the mandibular first molar region of twenty patients which were equally divided into two groups.

The Effect of Strontium Ranelate Gel on Bone Formation in Calvarial Critical Size Defects.

Aim: The current study was designed to investigate the effectiveness of locally applied Strontium ranelate to induce bone formation.

Materials And Methods: Forty-eight female rats were divided into six groups (eight rats in each group): The three test groups included Strontium (SR) 2.5 mg, 5 mg and 10 mg that was dissolved in methylcellulose gel.

Maxillary Sinus Augmentation Using a Titanium Mesh: A Randomized Clinical Trial.

Background: Various attempts have been implemented using different materials and techniques to augment the maxillary sinus floor for prospect dental implant positioning.

Aim: This contemplate was conducted to assess the osteogenic capability of the maxillary sinus in a two-step sinus membrane elevation using titanium mesh to keep the formed space to place dental implants in atrophic ridges.

Materials And Methods: Titanium micromesh was customized and positioned into the sinus on one side to preserve the elevated membrane in position.

Different modalities for treatment of recurrent aphthous stomatitis. A Randomized clinical trial.

Background: The underlying etiology of recurrent aphthous stomatitis (RAS) is unclear and treatment aims to provide symptomatic and faster relief. This study compared the efficacy of diode laser, a herbal combination of Acacia nilotica and Licorice (A and L) and Amlexanox in the management of RAS.

Material And Methods: Sixty patients with minor aphthae were selected and randomly divided into four groups of 15 each.

Characterization and Cytotoxicity Analysis of a Ciprofloxacin Loaded Chitosan/Bioglass Scaffold on Cultured Human Periodontal Ligament Stem Cells: a Preliminary Report.

Aim: The aim of this study was to analyze the cytotoxicity of ciprofloxacin (CIP) loaded on chitosan bioactive glass scaffold on human periodontal ligament stem cells (PLSCs) in vitro.

Materials And Methods: PLSCs obtained from human third molars, cultures treated with medium containing 15 x 15 mm chitosan/bioactive glass scaffolds without/with different concentration 0, 5, 10, and 20 % of CIP. A total of 15 x 10^3 cells were plated in 6 well plates.

Treatment of Gingival Hyperpigmentation by Diode Laser for Esthetical Purposes.

Background: Gingival hyperpigmentation is a common esthetical concern in patients with gummy smile or excessive gingival display. Laser ablation has been recognized recently as the most effective, pleasant and reliable technique. It has the advantage of easy handling, short treatment time, hemostasis, decontamination, and sterilization effect.

Evaluation of horizontal ridge augmentation using beta tricalcium phosphate and demineralized bone matrix: A comparative study.

Objectives: To evaluate the effectiveness of beta tricalcium phosphate (β-TCP) alone compared to β-TCP and Demineralized Bone Matrix (DBM) in regenerating localized horizontal maxillary alveolar ridge deficiencies prior to implant placement.

Study Design: The study included 20 patients with horizontal maxillary ridge deficiencies limited to one or more neighbouring teeth and initial ridge width of ≤ 5mmm. Patients were divided equally into two equal groups.

Periodontitis and cardiovascular disease: Floss and reduce a potential risk factor for CVD.

There is evidence supporting an association between cardiovascular disease (CVD) and periodontitis. We determined whether patients with chronic periodontitis, who are otherwise healthy individuals, have higher serum concentrations of emerging risk markers of CVD such as C-reactive protein (CRP) and interleukin 6 (IL-6) and investigated the effect of subsequent periodontal treatment on the levels of these markers. A total of 40 individuals were included in the study.