Glycerol is a promising low-cost solvent for biomass pretreatment since a large amount of glycerol is generated as a by-product in the biodiesel industry. Pretreatment is a method of disintegration of the recalcitrant structure of biomass to enhance the accessibility of cellulose and hemicelluloses to enzymes for complete saccharification. During pretreatment, glycerol breaks the lignin carbohydrate complex and selectively solubilizes lignin.
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