Digital Immunoassay for Rapid Detection of SARS-CoV-2 Infection in a Broad Spectrum of Animals.

The ability of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) to infect a wide-range of species raises significant concerns regarding both human-to-animal and animal-to-human transmission. There is an increasing demand for highly sensitive, rapid, and simple diagnostic assays that can detect viral infection across various species. In this study, we developed a biosensor assay that adapted a monoclonal-antibody (mAb)-based blocking ELISA format into an Activate Capture + Digital Counting (AC + DC)-based immunoassay.

Recommended approaches to assessing and managing physiotherapy clients experiencing psychological distress: a systematic mapping review.

Background: Some physiotherapists find assessing and managing clients experiencing psychological distress challenging and are uncertain regarding the boundaries of the profession's scope.

Objective: To map the approaches recommended for physiotherapists in scholarly literature, with respect to the assessment and management of clients experiencing psychological distress.

Methods: A systematic mapping review was conducted.

'Highlighting a gap in radiographers' pain knowledge: A letter to the editor on 'Radiographers' decision-making processes in the movement of pain-afflicted adult trauma patients in planar diagnostic imaging''.

This letter to the editor highlights a crucial knowledge gap within the radiography profession-a modern understanding of pain. The outdated belief that pain is directly correlated to tissue injury or pathology is misleading and potentially harmful, as it could lead to the stigmatisation and invalidation of patients with pain. Radiographers are urged to listen to their patients in order to truly understand their pain, and to embrace the opportunity to develop their knowledge and enhance the experience of patients in the imaging department.

Body condition of returning Atlantic salmon Salmo salar L. correlates with scale δC and δN content deposited at the last marine foraging location.

Patterns of feeding and growth of Atlantic salmon Salmo salar L. in the marine environment are critical to understanding how observed declines in recruitment may reflect warming or other oceanic drivers. The isotopic composition of scales can provide insight into differences in marine feeding location and possibly temperature regime.

The spawning location of vulnerable ferox trout (Salmo trutta L.) in the Lough Corrib and Lough Mask catchments, Western Ireland.

Ferox trout are large, long-lived, piscivorous trout normally found in deep lakes; they are highly prized by trophy anglers. Lough Corrib and Lough Mask, Western Ireland, have recorded the majority of Irish specimen ferox trout since angling records began. Little was known regarding the spawning location of ferox trout relative to sympatric brown trout, and a radio telemetry study was initiated in both catchments in 2005.

Non-native species and lake warming negatively affect Arctic char Salvelinus alpinus abundance; deep thermal refugia facilitate co-existence.

This study finds that non-native species and warming temperatures have significant negative effects on Arctic char Salvelinus alpinus abundance in Irish lakes. Eutrophication was not important at the range of total phosphorus tested (0.005-0.

Coexistence of pike Esox lucius and brown trout Salmo trutta in Irish lakes.

An environmental study of pike Esox lucius recorded their presence in 522 Irish lakes and that they coexisted with brown trout Salmo trutta in 97 of these. Statistical models, accounting for spatial non-independence among lakes, suggested that lakes with greater area, maximum depth and stream connectivity show a higher probability of coexistence. Introductions of E.

Marital disruption among women with genital fistula in Nigeria: who is at greatest risk?

Introduction And Hypothesis: Marital disruption is a commonly identified sequela of genital fistula in developing countries. This study is aimed at identifying factors that correlate with marital outcomes.

Methods: All new patients presenting to Evangel VVF Centre in Jos, Nigeria, between August 2015 and August 2017, were retrospectively reviewed with regard to demographics, medical history, and fistula details to identify variables correlating with marital status, particularly whether currently married or presently divorced, separated, or divorced and remarried (cumulatively the "divorce group").

Pubovesical sling for residual incontinence after successful vesicovaginal fistula closure: a new approach to an old procedure.

Introduction And Hypothesis: For decades, the pubovesical (PV) sling has been in the armamentarium of the fistula surgeon for treating persistent urinary incontinence after successful fistula closure. We report our early experience with slings, and then also introduce a new "tight" PV sling technique for management of post-fistula urethral leak. Our hypothesis is that performance of tight slings might result in improved continence for women with persistent urinary incontinence after obstetric fistula closure.

Effect of HIV infection on outcomes after surgical repair of genital fistula.

Objective: To compare outcomes following surgical repair of genital fistula among Nigerian women with or without HIV.

Methods: A retrospective review was conducted of all genital fistula repair surgeries performed at Evangel Vesicovaginal Fistula Center in Jos, Nigeria, between January 1, 2004, and April 30, 2014. Patient characteristics, HIV status, genital fistula characteristics, and postoperative outcomes were assessed for between-group variance.

Bladder stones in vesicovaginal fistula: is concurrent repair an option? Experience with 87 patients.

Introduction And Hypothesis: The objective was to assess the outcomes of a one-stage approach to bladder stones in the setting of a vesicovaginal fistula, performing fistula repair concurrently with stone extraction.

Methods: Retrospective review of urogenital fistula surgeries at Evangel VVF Center in Jos, Nigeria, between December 2003 and April 2014, identified 87 women with bladder stones accompanying their fistulas and 2,979 repaired without stones. Concurrent stone extraction and fistula repair were performed in 51 patients.

Temporal stability and rates of post-depositional change in geochemical signatures of brown trout Salmo trutta scales.

This study investigates temporal stability in the scale microchemistry of brown trout Salmo trutta in feeder streams of a large heterogeneous lake catchment and rates of change after migration into the lake. Laser-ablation inductively coupled plasma mass spectrometry was used to quantify the elemental concentrations of Na, Mg, Mn, Cu, Zn, Ba and Sr in archived (1997-2002) scales of juvenile S. trutta collected from six major feeder streams of Lough Mask, County Mayo, Ireland.

The path of least resistance: A case of cervical stenosis and uterocutaneous fistula.

Uterocutaneous fistula is exceedingly rare, and uniformly follows some type of operative procedure. In this case, a young woman underwent a cesarean delivery at an outlying clinic in rural Nigeria, following which she developed amenorrhea and cyclic pelvic pain. In attempts to resolve her condition, a second laparotomy was performed at the same medical center.

Thermal, trophic and metabolic life histories of inaccessible fishes revealed from stable-isotope analyses: a case study using orange roughy Hoplostethus atlanticus.

A time-resolved record of inhabited water depth, metabolic rate and trophic behaviour of the orange roughy Hoplostethus atlanticus was recovered from combined stable-isotope analyses of otolith and muscle tissue. The results demonstrate that H. atlanticus from the north-east Atlantic Ocean have a complex life history with three distinct depth-stratified life stages.

The missing foley catheter: an unusual finding in vesicouterine fistula.

A 28-year-old G1P1 presented complaining of urine leakage per vaginum following caesarean delivery, accompanied by amenorrhoea, cyclic haematuria and cyclic pelvic pain. Examination findings were suggestive of vesicouterine fistula and the patient was taken for exploratory laparotomy, during which the foley catheter could not be identified within the bladder. During separation of the bladder from the uterus, the catheter was found to be traversing the fistulous tract into the uterine cavity.

Spatial heterogeneity in fishing creates de facto refugia for endangered Celtic Sea elasmobranchs.

The life history characteristics of some elasmobranchs make them particularly vulnerable to fishing mortality; about a third of all species are listed by the IUCN as Threatened or Near Threatened. Marine Protected Areas (MPAs) have been suggested as a tool for conservation of elasmobranchs, but they are likely to be effective only if such populations respond to fishing impacts at spatial-scales corresponding to MPA size. Using the example of the Celtic Sea, we modelled elasmobranch biomass (kg h(-1)) in fisheries-independent survey hauls as a function of environmental variables and 'local' (within 20 km radius) fishing effort (h y(-1)) recorded from Vessel Monitoring Systems data.

Analytical approaches for addressing the variation in back-calculated age-length relationships for fish.

Estimating an age-length relationship is a routine aspect of many fisheries studies and is simplified by the use of commercially available computer programs. These computer programs may be misleading since a result can be produced irrespective of the quality or the extent of the data, and there is some concern that back-calculated age-length relationships are sensitive to the sample size and composition. We investigated this issue by comparing estimates of mean back-calculated lengths at age and growth rates derived from subsets of a large sample of wild channel catfish Ictalurus punctatus (N=788) collected in 2001 and 2002 from 9 rivers in Mississippi, United States.