Standardness of antimicrobial discs by various manufacturers in the light of import substitution.

In connection with the introduction of economic sanctions against the Russian Federation by the United States, the European Union, Japan, and a number of other countries, import substitution is becoming one of the strategic objectives of the Russian economy The comparative assessment of standardness and effectiveness of six antimicrobial commercial producer-varying disks for clinical and test strains of microorganisms has been conducted. It is based on analyzing of the variation coefficient (Cv) of inhibition zone diameters, mean values, and diameter values ranges, which are indicators of reproducibility, accuracy and efficiency, respectively. Discs from BD BBL result in insignificant variations of inhibition zone diameters, providing accurate and reproducible data.

Isolation and identification of listeria in clinical material.

Results from research on isolation, identification, and study of biological properties of L. monocytogenes clinical isolates and Listeria spp test strains are presented. Peculiarities of modern research methods for indicating and identifying pathogenic listeria to improve the quality of laboratory studies of clinical material are studied.

Comparative tests of quality of bacteriological investigation with Corynebacterium diphtheriae isolation.

The results of comparative experimental studies of identification of nontoxigenic C. diphtheriae strain by three different commercial laboratories are presented. A typical nontoxigenic strain of C.

Possibilities of practical application of different culture mediums for laboratory diagnostic of diphtheria.

The purpose of the work is to evaluate the cultural and morphological properties of colonies of clinically significant corynebacteria on culture mediums for the isolation of corynebacteria. The study used 9 culture mediums for the isolation of corynebacteria: a culture medium for the isolation of corynebacteria (Corynebacagar); Tellurite-containing blood agars on base - Culture medium № 1 GRM, Culture agar for the cultivation of microorganisms (GRM agar), Culture medium for determining the sensitivity of microorganisms to antibacterial preparations - AGV, culture agar for the cultivation of dry microorganisms (SPA), Clauberg medium II, Hoyle Medium agar (Oxoid), Blood agar base (Conda), Columbia Agar Base (Conda). The work used 7 test strains of microorganisms from the State collections of pathogenic microorganisms - C.

Efficiency of bacteriological transport media quality control.

The aim of the study is to assess the effectiveness of quality control of transport environments in accordance with the requirements of domestic and international standards using aerobic and optional anaerobic microorganisms. The results of a comparative assessment of the survival of 10 strains of aerobic and optional anaerobic microorganisms after storage in the semi-liquid transport environment of Ames for 24 and 48 hours at temperatures (4-8)0 C and (20-25)0 C in accordance with the requirements of domestic and international standards are presented. The methodology described in ICC 4.

Clinical trials of new culture media for staphylococcus isolation.

A comparative analysis of the quality of the developed nutrient media, Baird-Parker dry agar base and Vogel-Johnson dry agar Base and foreign analogues, was done based on results of clinical trials. The tested media were qualified by the main biological parameters, such as sensitivity, growth rate, and differentiating and inhibiting properties. The evaluation of statistical reliability of the results of trials of clinical samples was evaluated taking into account the number of parallel studies and the number of matches of the results of studies conducted by different performers.

[Phenotypic and molecular genetic properties of Escherichia coli clinical strains isolated from patients with urological diseases].

Objective: Microbiological and molecular genetic characterization resistance profiles of Escherichia coli strains isolated in a pilot single-center clinical study from patients of the urological department in Yaroslavl in 2016-2017.

Materials And Methods: Clinical strains of E. coli (n=18) were isolated from the urine of women aged 23-84 years.

[Experience of using TB test kit for the rapid drug susceptibility testing of M. tuberculosis.].

The results of the comparative testing of the susceptibility of M. tuberculosis clinical strains to isoniazid, streptomycin, rifampicin and ethambutol using the TB test kit, developed in SCRAMB, (Obolensk) and the absolute concentrations method; the TB test kit and the BACTEC MGIT 960 automated system are presented in the study. A total of 629 and 220 strains, respectively, were tested.

[Trials of the domestically produced nutrient medium «Agar Muller-Hinton II - Obolensk».].

The results of the comparative tests of the «Agar Muller-Hinton II - Obolensk» nutrient medium developed in SRCAMB, Obolensk, and the control nutrient medium imported «Mueller Hinton II Agar» are presented in the study. The susceptibility of bacterial clinical strains to antimicrobial agents (AMP) was determined by the disc diffusion method and the method of gradient diffusion (E-test). The carbapenemase activity of the strains carrying the carbapenemase genes was determined by CIM-test.

[Comparison of hemolytic activity and hemolytic toxin genes of Staphylococcus aureus clinical strains, isolated in Russia.].

Clinical strains of Staphylococcus aureus were tested for hemolytic activity on blood agar, in the PCR test and by analyzing the gene alleles of hemolytic toxins. The study analyzed the information content of the phenotypic determination of hemolytic activity to assess the pathogenic properties of S. aureus isolates.

[Clinical trials of salmonella enrichment medium.].

Salmonella infections continue to be a serious problem in modern medicine. Being intestinal infections-associated pathogens, representatives of the genus Salmonella manifest themselves as pathogenic bacteria, especially in developing nosocomial infections. Given the polymorphism of clinical symptoms of salmonellosis, laboratory studies using bacteriological and serological methods are an important link in the diagnosis.

[The characteristic of diarrheagenic Escherichia separated from children aged under 5 years old in Yaroslavl.].

The diarrheagenic bacteria coli take a significant place among agents of acute intestinal infections in children aged under 5 years. The main danger among these pathogens is represented by both enterotoxigenic E. coli causing enteritis and enterocolitis accompanied by acute dehydration diarrhea and Escherichia producing shiga-toxin being agents of hemorrhagic colitis and hemolytic uremic syndrome.

[The clinical trials of agar and Mossel EE broth.].

The cultural diagnostic of acute intestinal infections is based on application of growth medium for selective cumulation of enterobacteria. The development of composition and technology of production of national import-substituting growth medium is an important task for supporting laboratories with high quality medical products. In the state research center of applied microbiology and biotechnology are developed and registered in established procedure a dry growth medium for selective cumulation of enterobacteria (Mossel EE broth) and dry growth medium for selective isolation and counting of enterobacteria (Mossel agar).

[TMOSKOVHE COMPARATIVE CHARACTERISTIC OF GROWTH MEDIUMS FOR SEPARATION OF CORYNEBACTERIA].

The comparative tests of growth mediums for isolation and accumulation of diphtheria bacteria were implemented. The testing consisted of six series of growth medium "Corynebacagar" produced by the state research center of applied microbiology and biotechnology and three series of blood tellurite agar. The concluding results of identification of biological indicators of all series of growth nutrient mediums are presented The "Corynebacagar" is recommended for application in health care practice for primary inoculation of pathological material during implementation of cultural analysis on diphtheria.

[THE CONDITION AND TENDENCIES OF DEVELOPMENT OF CLINICAL AND SANITARY MICROBIOLOGY IN THE RUSSIAN FEDERATION AND PROBLEM OF IMPORT SUBSTITUTION].

The import substitution becomes one of the strategic tasks of national economy as a result of prolongation of economic sanctions concerning the Russian Federation of part of the USA, EU countries, Japan and number of other countries. It is not proper to be limited in import substitution only by goods because in conditions ofsanctions when access toforeign technologies is complicated Russia is needed to substitute foreign technologies by national designs in faster manner One of directions of effective import substitution is localization of production of laboratory equipment and consumables for clinical and sanitary microbiology on the territory ofthe Russian Federation and countries of Customs union. In Russia, in the field ofdiagnostic of dangerous and socially significant infections, all components for import substitution to implement gene diagnostic, immune diagnostic.

[THE NATIONAL NUTRIENT MEDIUM FOR DIAGNOSTIC OF PURULENT BACTERIAL MENINGITIS].

The national growth mediums were developed for isolating and cultivating of main agents of purulent bacterial meningitis--haemophilus agar, chocolate agar, PBM-agar. The growing and selective characteristics of developed growth mediums are examined. The haemophilus agar ensures growth of Haemophilus influenzae.

[THE ACTUAL APPROACHES TO PROBLEM OF IMPORT SUBSTITUTION IN TH FIELD OF PRODUCTION GROWTH MEDIUM].

The import substitution becomes one of strategic tasks of Russian economy as a result of imposition of economic sanctions on part of the USA, EU countries, Japan and number of other states. The development of structure and technology of production of national import substituted growth mediums permits satisfying needs of laboratory service of Russia inactive storage and to secure appropriate response to occurring challenges and new biological menaces and support bio-security of state at proper level. The presented data concerning substantiation of nomenclature of growth mediums and transport system permit satisfying in fullness the needs of clinical and sanitary microbiology in growth mediums of national production and to give up of import deliveries without decreasing of quality of microbiological studies.

[The comparative evaluation of differential diagnostic characteristics of Endo growth medium of different manufacturers].

The article deals with the results of comparative study of differential diagnostic characteristics of Endo growth medium of different foreign manufacturers permitted to be applied in the Russian Federation and the national Endo agar. The enhanced kit of museum test-strains and clinical material from patients were used. It is established that morphology, size and number of clumps of most enterobacteria growing in Tndo medium manufactured by Merck, Pronadisa, HiMedia and the state research center of applied microbiology and biotechnology have minor morphological differences and match the characteristics declared by manufacturers.