Construction of T7-Like Expression System in KT2440 to Enhance the Heterologous Expression Level.

KT2440 has become an attractive chassis for heterologous expression with the development of effective genetic manipulation tools. Improving the level of transcriptional regulation is particularly important for extending the potential of KT2440 in heterologous expression. Although many strategies have been applied to enhance the heterologous expression level in KT2440, it was still at a relatively low level.