Effects of N-linked glycosylation on the enzymatic properties of GH12 bifunctional enzymes from Aspergillus terreus expressed in Pichia pastoris.

In industry, bioenergy, food process, and feed application, endoglucanases are highly valuable for lignocellulose degradation with high catalytic activity under high temperatures. The glycoside hydrolase family 12 endoglucanase (AtEglD) from Aspergillus terreus can efficiently hydrolyze both β-glucan and xyloglucan of barley with an optimal temperature of 55 °C under pH 5.0.