Publications by authors named "Shengzhen Chen"
Background: Pineapple (Ananas comosus L.) is a major tropical fruit crop with considerable economic importance, and its growth and development are significantly impacted by low temperatures. The plant-specific GRAS gene family plays crucial roles in diverse processes, including flower and fruit development, as well as in stress responses.
View Article and Find Full Text PDFPassion fruit, an economically valuable fruit crop, is highly vulnerable to adverse climate conditions. The genes, recognized as abscisic acid (ABA) and stress-inducible, play vital roles in stress response and growth regulation in diverse eukaryotic organisms. Here, six genes were firstly identified in passion fruit genome and all predicted to be localized within the endoplasmic reticulum.
View Article and Find Full Text PDFArticle Synopsis
- Passion fruit is a valuable tropical fruit facing challenges in growth due to climate change, emphasizing the need for research on its resilience.
- The study identified 90 antioxidant enzyme genes in passion fruit, revealing their diverse expression patterns related to stress protection and development.
- Regulatory factors such as miRNAs and transcription factors play a complex role in these antioxidants, paving the way for future breeding strategies to enhance temperature stress resistance.
[Construction of prokaryotic expression vector of HPV16E6 gene and its expression].
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
August 2010
Prokaryotic expression vector of mouse HPV16E6 gene was constructed. A pair of primers were designed according to the digestion sites in plasmid pGEX-KG and the HPV16E6 gene sequence published by GenBank. The DNA fragment of 321bp was amplified by PCR from the HPV recombinant plasmid with HPV16E6 gene, then cloned into pGEX-KG and transformed into the host E.
View Article and Find Full Text PDF[Prokaryotic expression of HPV 16L1 and optimization of expression conditions].
Zhejiang Da Xue Xue Bao Yi Xue Ban
July 2010
Objective: To construct the HPV16 L1 prokaryotic expression plasmid and to optimize its expression.
Methods: A pair of primers was designed according to plasmid sequences of pGEX-KG and the HPV16L1 genes published by GeneBank. The DNA fragment of 1500 bp was amplified by PCR from the HPV recombinant plasmid with HPV16L1 gene, then cloned into pGEX-KG and transformed into the host E.