Long-term stable water-in-oil-in-water emulsion for effective protection and sustained release of lysine-calcium using chitosan and hydroxypropyl methyl cellulose.

The poor tolerance to gastric acid and low absorption of calcium supplements in the intestinal tract remain a serious limitation in applications. Herein, lysine-calcium (Lys-Ca) has been synthesized via the chelation of Lys and high-temperature calcination scallop shell powder (HCSP), and subsequently encapsulated in a carefully designed water-in-oil-in-water (W/O/W) emulsion with a high encapsulation efficiency of 93 % using chitosan (CS) and hydroxypropyl methylcellulose (HPMC). Owing to the interfacial film formed by CS and HPMC between the droplets, the resulting emulsion demonstrates good acid and thermal stability, as well as long-term stability even after 60 d of storage at 25 °C.

Complete genome sequence of a novel sp. R16 isolate from deep-sea sediments in the western Pacific.

, a distinct genus separate from , has garnered attention for its adaptability and ecological versatility. In this study, a Gram-stain positive and ovoid-rod shaped the actinobacterium strain R16 was isolated from deep-sea sediment (with a depth of 6,310 m) in the Western Pacific. On the basis of 16S rRNA gene sequence analysis, average nucleotide identity and phylogenomic analysis, strain R16 clearly represents a novel species within the genus .

Microcella flavibacter sp. nov., isolated from marine sediment, and reclassification of Chryseoglobus frigidaquae, Chryseoglobus indicus, and Yonghaparkia alkaliphila as Microcella frigidaquae comb. nov., Microcella indica nom. nov., and Microcella alkalica nom. nov.

A novel Gram-staining positive, aerobic, rod-shaped, non-motile and yellow-pigmented actinobacterium, designated strain WY83, was isolated from a marine sediment of Indian Ocean. Strain WY83 grew optimally at 30-35 °C, pH 7-8 and with 0-3% (w/v) NaCl. The predominant menaquinones were MK-10, MK-11 and MK-12, and the major fatty acids were C ω9c/C19:1 ω11c, anteiso-C, C 3OH, and iso-C.

Nesterenkonia sedimenti sp. nov., isolated from marine sediment.

A Gram-staining-positive actinobacteria strain, designated MY13, was isolated from deep-sea sediment of the western Pacific Ocean and subjected to a taxonomic polyphasic investigation. Based on the results, cells were aerobic, irregular short rod, non-motile and non-spore-forming. Colonies were cream, circular, smooth, convex, opaque and 1.

Description of Microbacterium luteum sp. nov., Microbacterium cremeum sp. nov., and Microbacterium atlanticum sp. nov., three novel C50 carotenoid producing bacteria.

We have identified three Microbacterium strains, A18JL200, NY27, and WY121, that produce C50 carotenoids. Taxonomy shows they represent three novel species. These strains shared < 98.

Brevibacterium limosum sp. nov., Brevibacterium pigmenatum sp. nov., and Brevibacterium atlanticum sp. nov., three novel dye decolorizing actinobacteria isolated from ocean sediments.

During a study of the marine actinobacterial biodiversity, a large number of Brevibacterium strains were isolated. Of these, five that have relatively low 16S rRNA gene similarity (98.5-99.

A rapid and efficient method for the extraction and identification of menaquinones from Actinomycetes in wet biomass.

Background: Menaquinones are constituents of prokaryote cell membranes where they play important functions during electron transport. Menaquinone profiles are strongly recommended for species classification when proposing a new Actinomycetes taxon. Presently, the most widely used methods to determine menaquinones are based on freeze-dried cells.

Acremolin D, a new acremolin alkaloid from the deep-sea sediment derived fungus.

Chemical investigation of the deep-sea-derived fungus MCCC 3A00324 led to the isolation of one new acremolin type alkaloid (acremolin D, ) and five known alkaloids (). The planar structure of was established by the extensive analyses of the NMR and HRESIMS data, while its absolute configuration was assigned by the comparison of the experimental and calculated ECD data. Acremolin D () represented the second analogue of acremolin found in nature.

Complete genome sequence of Streptomyces sp. HSG2 from rhizosphere soil of mangrove in Qingmei Gang, Sanya.

Streptomyces sp. HSG2 was isolated from rhizosphere soil of a mangrove forest sample at Qingmei Gang, Sanya. The complete genome sequence of the strain HSG2 was obtained using PacBio Sequel HGAP.

sp. nov., isolated from mangrove sediments.

A novel Gram-staining-negative, catalase- and oxidase-positive, facultatively anaerobic and rod-shaped motile bacterial strain, designated as ZWAL4003, was isolated from mangrove sediments of the Zini Mangrove Forest, Zhangzhou City, PR China. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that ZWAL4003 was grouped into a separated branch with MSSRF60 (97.38% nucleotide sequence identity) and NBRC 103148 (97.

Complete genome sequence of Janibaecter indicus YB324 from an Atlantic marine sediment.

Janibacter indicus YB324, a gram-positive, aerobic and non-motile actinobacterium isolated from south Atlantic sediment at a depth of 2875 m. The complete genome sequence of the strain YB324 was obtained using PacBio Sequel HGAP.4 and comprised of 3,369,845 base pairs with a 71.

New Monoterpenoids and Polyketides from the Deep-Sea Sediment-Derived Fungus MCCC 3A00324.

Chemical study of the secondary metabolites of a deep-sea-derived fungus MCCC 3A00324 led to the isolation of eleven compounds (-), including one novel () and one new () osmane-related monoterpenoids and two undescribed polyketides ( and ). The structures of the metabolites were determined by comprehensive analyses of the NMR and HRESIMS spectra, in association with quantum chemical calculations of the C NMR, ECD, and specific rotation data for the configurational assignment. Compound possessed a novel monoterpenoid skeleton, biogenetically probably derived from the osmane-type monoperpenoid after the cyclopentane ring cleavage and oxidation reactions.

sp. nov., isolated from deep sea water.

A novel Gram-stain-positive, motile, aerobic, non-spore-forming and slender rod-shaped actinobacterium, designated strain CTD02-10-2, was isolated from deep sea water of the Indian Ocean. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain CTD02-10-2 was grouped into a separated branch with JCM 14730 (98.4 % nucleotide sequence identity).

Phenolic bisabolane and cuparene sesquiterpenoids with anti-inflammatory activities from the deep-sea-derived Aspergillus sydowii MCCC 3A00324 fungus.

Seventeen undescribed sesquiterpenoids including 14 phenolic bisabolanes, namely asperbisabolanes A-N (1-14), and 3 cuparenes (aspercuparenes A-C, 15-17), together with 10 known bisabolane analogues (18-27) were isolated from the EtOAc extract of fermented cultures of the deep sea sediment-derived fungus Aspergillus sydowii MCCC 3A00324. The new structures were established on the basis of extensive NMR and HRESIMS spectroscopic data analyses, while their absolute configurations were assigned by comparison of the experimental ECD spectra with those of the TDDFT-ECD calculated spectra or reported data in literature. Asperbisabolanes A (1) and B (2) are the first examples of bisabolane sesquiterpenoids featuring a 6/6/6 tricyclic nucleus.

sp. nov., isolated from deep-sea sediment of the Western Pacific Ocean.

A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1, was isolated from a deep-sea sediment of the Western Pacific Ocean. Strain O1 showed optimal growth at 30 °C, between pH 6.0 and 8.

sp. nov. a novel actinobacterium isolated from the stony coral .

A novel Gram-stain-positive, aerobic, non-motile actinobacterium, designated strain E2A, was isolated from a coral sample and examined using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E2A formed a distinct phyletic lineage in the genus and was closely related to CCTCC AA 2012022 (96.4 %) and CCTCC AA 2010012 (95.

sp. nov. and sp. nov., isolated from the Southern Atlantic Ocean.

Two Gram-positive, aerobic, non-motile and non-spore-forming actinobacteria, strains GY074 and GY239, were isolated from deep-sea sediment of the Southern Atlantic Ocean. The results of phylogenetic analysis of 16S rRNA gene sequences placed both isolates within the genus , and showed a sequence similarity of 98.3 % between the two strains and similarites of 94.

Paracoccus subflavus sp. nov., isolated from Pacific Ocean sediment.

A Gram-stain-negative, short rod-shaped, non-motile, catalase- and oxidase-positive, aerobic bacterium, designated GY0581, was isolated from marine sediment sampled from the Western Pacific Ocean. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GY0581 belonged to the genus Paracoccus and had the highest levels of sequence similarity to Paracoccus sediminis JCM 18467 (98.2 %).