Free-Standing Lipid Bilayers Based on Nanopore Array and Ion Channel Formation.

Integrated nanopores are novel and versatile single-molecule sensors for individual label-free biopolymer detection and characterization. However, their studies and application requires a stable lipid bilayer to maintain protein function. Herein, we describe a method for producing lipid bilayers across a nanopore array on a silicon nitride substrate.

In vitro inhibition of tumor growth by low-dose iron oxide nanoparticles activating macrophages.

Macrophages as immunocyte are attracting more and more attention in cancer therapy. Our previous study observed that dimercaptosuccinic acid (DMSA)-coated FeO magnetic nanoparticles triggered comprehensive immune responses of mouse macrophages (RAW264.7 cells) and induced production of many kinds of cytokines.

Effect of NbO doping on improving the thermo-mechanical stability of sealing interfaces for solid oxide fuel cells.

NbO is added to a borosilicate sealing system to improve the thermo-mechanical stability of the sealing interface between the glass and Fe-Cr metallic interconnect (Crofer 22APU) in solid oxide fuel cells (SOFCs). The thermo-mechanical stability of the glass/metal interface is evaluated experimentally as well as by using a finite element analysis (FEA) method. The sealing glass doped with 4 mol.

Detection of a single enzyme molecule based on a solid-state nanopore sensor.

The nanopore sensor as a high-throughput and low-cost technology can detect a single molecule in a solution. In the present study, relatively large silicon nitride (Si3N4) nanopores with diameters of ∼28 and ∼88 nm were fabricated successfully using a focused Ga ion beam. We have used solid-state nanopores with various sizes to detect the single horseradish peroxidase (HRP) molecule and for the first time analyzed single HRP molecular translocation events.

Single Nanoparticle Translocation Through Chemically Modified Solid Nanopore.

The nanopore sensor as a high-throughput and low-cost technology can detect single nanoparticle in solution. In the present study, the silicon nitride nanopores were fabricated by focused Ga ion beam (FIB), and the surface was functionalized with 3-aminopropyltriethoxysilane to change its surface charge density. The positively charged nanopore surface attracted negatively charged nanoparticles when they were in the vicinity of the nanopore.

DNA-functionalized silicon nitride nanopores for sequence-specific recognition of DNA biosensor.

Nanopores have been proven to be novel and versatile single-molecule sensors for individual unlabeled biopolymer detection and characterization. In the present study, a relatively large silicon nitride (Si3N4) nanopore with a diameter of approximately 60 nm was fabricated successfully using a focused Ga ion beam (FIB). We demonstrated a simple ex situ silanization procedure to control the size and functionality of solid-state nanopores.

A novel biosensor based on sol-gel poly () (PVA)/(titanium dioxide)TiO hybrid material.

A novel hydrogen peroxide biosensor has been fabricated based on Hb entrapped poly(vinyl alcohol) (PVA)/Titanium dioxide (TiO2) hybrid material. Multi-walled carbon nanotubes (MWCNTs) were then dispersed into the composite matrix. It was found that such hybrid material could retain the native biocatalytic activity of the entrapped Hb by electrochemical experiments.

Amperometric hydrogen peroxide biosensor based on immobilization of hemoglobin on a glassy carbon electrode modified with fe(3)o(4)/chitosan core-shell microspheres.

Novel magnetic Fe(3)O(4)/chitosan (CS) microspheres were prepared using magnetic Fe(3)O(4) nanoparticles and the natural macromolecule chitosan. Then, using an easy and effective hemoglobin (Hb) immobilization method, an innovative biosensor with a Fe(3)O(4)/CS-Hb-Fe(3)O(4)/CS "sandwich" configuration was constructed. This biosensor had a fast (less than 10 s) response to H(2)O(2) and excellent linear relationships were obtained in the concentration range of 5.