Clinical and Genetic Characteristics of Early-Onset Lung Adenocarcinoma in a Large Chinese Cohort.

Background: The characteristics of early-onset lung adenocarcinoma (EOLA) have not been extensively studied. Our research aimed to comprehensively assess the clinical and genetic features of EOLA.

Methods: We conducted a retrospective analysis of surgically resected lung adenocarcinoma patients, categorizing them into the EOLA group (aged <40 years) and the late-onset lung adenocarcinoma (LOLA) group (aged >60 years).

Do Maternal Microbes Shape Newborn Oral Microbes?

Strong evidence suggests that the early composition of the oral microbiota of neonates plays an important role for the postnatal development of the oral health or immune system. However, the relationship between the maternal microbiome and the initial neonatal microbiome remains unclear. In this study, 25 pregnant women and their neonates were recruited, and the samples were collected from the maternal oral cavity, amniotic fluid, placenta and neonatal oral cavity.

Bioinformatics analysis of aberrantly expressed exosomal lncRNAs in oral squamous cell carcinoma (CAL-27 vs. oral epithelial) cells.

Oral squamous cell carcinoma (OSCC) is the most prevalent form of malignant tumour in the oral cavity and its early detection is critical for improving the prognosis of affected patients. The present study aimed to isolate and confirm exosomes derived from the supernatant of the OSCC cell line CAL-27 and human oral epithelial cells (HOECs), analyze long non-coding RNA (lncRNA) expression profiles and determine the diagnostic value based on bioinformatics analyses. The results indicated that the particles isolated from the supernatant of CAL-27 and HOECs were either round or oval, had a size range of 30-150 nm and were enriched with ALG-2 interacting protein X (ALIX) and tumour susceptibility 101 proteins (TSG101).

Exploring potential biomarkers for lung adenocarcinoma using LC-MS/MS metabolomics.

Background: The average 5-year survival rate of lung adenocarcinoma patients is only 15% to 17%, which is primarily due to late-stage diagnosis and a lack of specific prognostic evaluations that can recommend effective therapies. Additionally, there is no clinically recognized biomarker that is effective for early-stage diagnosis.

Methods: Tissue samples from 10 lung adenocarcinoma patients (both tumor and non-tumor tissues) and 10 benign lung tumor samples were collected.

[Construction of human telomerase reverse transcriptase periodontal ligament cell line mediated by adenovirus].

Objective: This study aims to establish an effective and stable periodontal ligament cell line stably expressing human telomerase reverse transcriptase (hTERT) gene by using the adenovirus method.

Methods: Polymerase chain reaction (PCR) was used to amplify the full length of hTERT gene to construct recombinant adenovirus plasmid pAd-pshuttle-cmv-hTERT. Packaged adenovirus particles were used for infection of human periodontal ligament cells.

Comparison of Periodontal Ligament Cell Lines with Adenovirus- and Lentivirus-Mediated Human Telomerase Reverse Transcription Expression.

The aims of this study were to generate periodontal ligament (PDL) cells that have adenovirus- or lentivirus-mediated overexpression of human telomerase reverse transcriptase () and to compare the osteogenic and proliferative abilities of the two cell lines to establish an efficient and stable cell model that will be more suitable for studies of PDL regeneration. After construction of the recombinant adenovirus plasmid pAd-pshuttle-cmv-hTERT, human PDL cells were infected by packaged adenovirus and lentivirus particles to establish two PDL cell lines. The expression levels of and mRNA for alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, core-binding factor (runt-related transcription factor 2), and type I collagen were assessed for each cell line.

Mechanism study on a plague outbreak driven by the construction of a large reservoir in southwest china (surveillance from 2000-2015).

Background: Plague, a Yersinia pestis infection, is a fatal disease with tremendous transmission capacity. However, the mechanism of how the pathogen stays in a reservoir, circulates and then re-emerges is an enigma.

Methodology/principal Findings: We studied a plague outbreak caused by the construction of a large reservoir in southwest China followed 16-years' surveillance.

A Facile Green Synthesis of Silver Nanoparticles Based on Poly-L-Lysine.

In this paper, we report a novel synthesis method for silver nanoparticles with facilely controllable morphology under mild conditions. The particles were synthesized by reducing silver nitrate in the presence of water soluble polymer of poly-L-Lysine (PLL) which was used as reducing agent and stabilizer. Experimental results revealed that size variation and overall formation mechanism of the resulting silver particles were mostly determined by the conformation of PLL which was in turn controlled by pH of solution and temperature.

Poly-L-Lysine Mediated Synthesis of Gold Nanoparticles and Biological Effects.

By using poly-L-lysine (PLL) as reducing agent and stabilizer, a novel method for synthesizing gold nanoparticles (GNP) under mild temperature conditions was reported. The morphology and size of these GNP could be easily controlled by changing the amount of PLL and temperature. And the GNPs prepared by this method had better stability than traditional methods.

[Analysis of epidemic situation of malaria in Longlin Various Nationalities Autonomous County in Guangxi from 1951 to 2014].

Objective: To analyze the data of epidemic situation of malaria in Longlin Various Nationalities Autonomous County, Guangxi Zhuang Autonomous Region, China from 1951 to 2014 and discuss the distribution characteristics and epidemic law, so as to provide the evidences for developing the prevention and control strategies of the disease.

Methods: The data about the epidemic situation of malaria in Longlin Various Nationalities Autonomous County were collected and analyzed statistically with Excel 2007.

Results: Totally 93 460 malaria cases were reported from 1951 to 2014 in the county.

A randomized controlled trial: preoperative home-based combined Tai Chi and Strength Training (TCST) to improve balance and aerobic capacity in patients with total hip arthroplasty (THA).

Objectives: To investigate the effect of a 12-week balance training program, combined TCST, on balance activity and aerobic capacity in patients with hip osteoarthritis.

Methods: Single-blind randomized grouping cohort study was conducted at our hospital from December 2008 to December 2011. A total of 81 patients aged from 60 to 69 years old diagnosed with end-stage hip osteoarthritis were recruited.

[Epidemiological investigation of imported falciparum malaria in Longlin County, Guangxi Zhuang Autonomous Region].

Among 41 people returned from Equatorial Guinea, 16 persons were infected with Plasmodium falciparum (39.02%) in Longlin County, Guangxi Zhuang Autonomous Region from October 2011 to February 2012. Among the 16 cases, Plasmodiumfalciparum was found in their blood smears of 15 cases, and 1 patient was diagnosed clinically as falciparum malaria.