A Phosphate-Starvation Enhanced Purple Acid Phosphatase, GmPAP23 Mediates Intracellular Phosphorus Recycling and Yield in Soybean.

Plant internal phosphorus (P) recycling is a complex process, which is vital for improving plant P use efficiency. However, the mechanisms underlying phosphate (Pi) release from internal organic-P form remains to be deciphered in crops. Here, we functionally characterised a Pi-starvation responsive purple acid phosphatase (PAP), GmPAP23 in soybean (Glycine max).

Modeling the recent drought and thinning impacts on energy, water and carbon fluxes in a boreal forest.

Globally, boreal forests act as important carbon sinks, however, drought and forest management could substantially alter the sink strength, though the controlling mechanisms of drought and management remain unclear. In this study, we combined the detailed process-based CoupModel with multiple measurements to study the impacts of recent drought and forest thinning on a boreal forest during 2018-2021. CoupModel after calibration showed high ability to represent the dynamics of long-term net ecosystem exchange and its responses to environmental changes.

The Stylo Cysteine-Rich Peptide Is Involved in Aluminum Tolerance through Enhancing Reactive Oxygen Species Scavenging.

Stylo ( spp.) is an important pasture legume with strong aluminum (Al) resistance. However, the molecular mechanisms underlying its Al tolerance remain fragmentary.

The object as the unit for state switching in visual working memory.

This study aimed to determine the unit for switching representational states in visual working memory (VWM). Two opposing hypotheses were investigated: (a) the unit of switching being a feature (feature-based hypothesis), and (b) the unit of switching being an object (object-based hypothesis). Participants (N = 180) were instructed to hold two features from either one or two objects in their VWM.

Dynamic changes in the aggregation-depolymerization behavior of Ovomucin-Complex and its binding to urease during in vitro simulated gastric digestion.

Ovomucin-Complex extracted from egg white is expected to have a barrier function similar to gastric mucin. In this study, the dynamic changes in structure, rheological properties and binding ability of Ovomucin-Complex during in vitro simulated gastric digestion were investigated. The results from HPLC and CLSM showed that extremely acidic pH (pH = 2.

Genipin improves obesity through promoting bile secretion and changing bile acids composition in diet-induced obese rats.

Objectives: Bile acids (BAs), as signaling molecules to regulate metabolism, have received considerable attention. Genipin is an iridoid compound extracted from Fructus Gradeniae, which has been shown to relieve adiposity and metabolic syndrome. Here, we investigated the mechanism of genipin counteracting obesity and its relationship with BAs signals in diet-induced obese (DIO) rats.

Nursing Based on Humanistic Care Concept for Continuous Blood Purification for Patients with Severe Sepsis in the Intensive Care Unit.

Context: Mortality from severe sepsis has been declining in recent years but remains a challenge worldwide because it remains the most frequent cause of death in ICUs. High-quality nursing care during a patient's CBP can play an important role in promoting a patient's physical condition.

Objective: The study intended to explore the effects of nursing based on a humanistic care concept on continuous blood purification (CBP) treatment for patients with severe sepsis in an intensive care unit (ICU).

Genome-wide identification of MAPK family in papaya (Carica papaya) and their involvement in fruit postharvest ripening.

Background: Papaya (Carica papaya) is an economically important fruit cultivated in the tropical and subtropical regions of China. However, the rapid softening rate after postharvest leads to a short shelf-life and considerable economic losses. Accordingly, understanding the mechanisms underlying fruit postharvest softening will be a reasonable way to maintain fruit quality and extend its shelf-life.

Kaempferol and nicotiflorin ameliorated alcohol-induced liver injury in mice by miR-138-5p/SIRT1/FXR and gut microbiota.

Aims: Excessive alcohol consumption can lead to alcoholic liver diseases (ALDs). is a rare Chinese medicinal herb. has been validated to be highly effective for treating hepatitis.

Long term all-cause mortality after myocardial infarction with non-obstructed vs obstructed coronary artery disease: a meta-analysis of adjusted data.

Background: The difference in the long-term outcomes of myocardial infarction in patients with non-obstructed coronary arteries (MINOCA) and patients with myocardial infarction with obstructed coronary artery disease (MI-CAD) is not clear. The current study aimed to pool adjusted data to compare long-term outcomes of MINOCA vs MI-CAD.

Methods: Electronic literature search of PubMed, Embase, CENTRAL, and Google Scholar databases was done for publications up to 18th June 2023.

Celastrol inhibits angiogenesis and the biological processes of MDA-MB-231 cells via the DEGS1/S1P signaling pathway.

Celastrol (Cel) shows potent antitumor activity in various experimental models. This study examined the relationship between Cel's antivascular and antitumor effects and sphingolipids. CCK-8 assay, transwell assay, Matrigel, PCR-array/RT-PCR/western blotting/immunohistochemistry assay, ELISA and HE staining were used to detect cell proliferation, migration and invasion, adhesion and angiogenesis, mRNA and protein expression, S1P production and tumor morphology.

Impaired glycosylation of GmPAP15a, a root-associated purple acid phosphatase, inhibits extracellular phytate-P utilization in soybean.

Phosphorus (P) is an essential nutrient, but easily fixed in soils. Therefore, most of soil P exists in the form of inaccessible organic phosphorus (Po), particularly phytate-P. Root-associated purple acid phosphatases (PAPs) are considered to play a crucial role in phosphate (Pi) scavenging in soils.

GPCR activation and GRK2 assembly by a biased intracellular agonist.

Phosphorylation of G-protein-coupled receptors (GPCRs) by GPCR kinases (GRKs) desensitizes G-protein signalling and promotes arrestin signalling, which is also modulated by biased ligands. The molecular assembly of GRKs on GPCRs and the basis of GRK-mediated biased signalling remain largely unknown owing to the weak GPCR-GRK interactions. Here we report the complex structure of neurotensin receptor 1 (NTSR1) bound to GRK2, Gα and the arrestin-biased ligand SBI-553.

Exploring the binding effect and mechanism of glycyrrhizin to ovomucin by combining spectroscopic analysis and molecular docking.

Ovomucin (OVM) is an ideal natural macromolecular glycoprotein extracted from eggs with good adhesion. Based on the defect that glycyrrhizin (GL) has good antiviral activity but fast metabolism, this study aimed to explore the binding effect and mechanism of GL to OVM, using multi-spectroscopic techniques, isothermal titration calorimetry (ITC), and molecular docking. The adhesion ability of OVM to the hydrophilic interface and GL was first demonstrated by dual polarization interferometry (DPI) analysis and binding capacity assay, and the OVM-GL complex exhibited a similar affinity for the spike protein of COVID-19.

Enhanced biohydrogen yield and light conversion efficiency during photo-fermentation using immobilized photo-catalytic nano-particles.

Bacterial immobilization is a common method in anaerobic fermentation, since of the maintenance of high bacterial activity, insurance of high density microbial during continuous fermentation, and quick adaptability to the environment. While, the bio-hydrogen production capacity of immobilized photosynthetic bacteria (I-PSB) is seriously affected by the low light transfer efficiency. Hence, in this study, photo-catalytic nano-particles (PNPs) was added into the photo-fermentative bio-hydrogen production (PFHP) system, and its enhancement effects of bio-hydrogen production performance were investigated.

Structural basis of peptide recognition and activation of endothelin receptors.

Endothelin system comprises three endogenous 21-amino-acid peptide ligands endothelin-1, -2, and -3 (ET-1/2/3), and two G protein-coupled receptor (GPCR) subtypes-endothelin receptor A (ETR) and B (ETR). Since ET-1, the first endothelin, was identified in 1988 as one of the most potent endothelial cell-derived vasoconstrictor peptides with long-lasting actions, the endothelin system has attracted extensive attention due to its critical role in vasoregulation and close relevance in cardiovascular-related diseases. Here we present three cryo-electron microscopy structures of ETR and ETR bound to ET-1 and ETR bound to the selective peptide IRL1620.

Identification and characterisation of the haemozoin of Haemonchus contortus.

Background: Most haematophagous organisms constantly suck the host's haemoglobin, which produces toxic free haem. This toxic haem aggregation into the nontoxic crystallisation complex known as haemozoin represents one of the most important detoxification pathways in living organisms, but very little is known about the features of haemozoin in parasitic nematodes. Here, we identified and characterised the haemozoin of an economically significant blood-sucking nematode, Haemonchus contortus.

Co-production process optimization and carbon footprint analysis of biohydrogen and biofertilizer from corncob by photo-fermentation.

In this study, corncob was taken as substrate, the co-production process of biohydrogen and biofertilizer by photo-fermentation was investigated and its carbon footprint analysis was conducted to evaluate the carbon transfer pathway. Biohydrogen was produced by photo-fermentation, and the hydrogen producing residues were immobilized by sodium alginate. Cumulative hydrogen yield (CHY) and nitrogen release ability (NRA) was taken as references, and the effect of substrate particle size on the co-production process was evaluated.

Improving phosphorus acquisition efficiency through modification of root growth responses to phosphate starvation in legumes.

Phosphorus (P) is one of the essential macronutrients for plant growth and development, and it is an integral part of the major organic components, including nucleic acids, proteins and phospholipids. Although total P is abundant in most soils, a large amount of P is not easily absorbed by plants. Inorganic phosphate (Pi) is the plant-available P, which is generally immobile and of low availability in soils.

Ascorbic acid-mediated zero-valent iron enhanced hydrogen production potential of bean dregs and corn stover by photo fermentation.

Ascorbic acid was introduced to enhance the performance of zero-valent iron (Fe(0)) in hydrogen production by photo fermentation of bean dregs and corn stover. The highest hydrogen production of 664.0 ± 5.

Preparation of microgel co-loaded with nuciferine and epigallocatechin-3-gallate for the regulation of lipid metabolism.

This study aims to enhance the stability and bioavailability of nuciferine (NF) and epigallocatechin-3-gallate (EGCG) by loading NF into liposomes and then incorporating the liposomes and EGCG into porous microgels (NFEG-microgel) prepared with chitosan and proanthocyanidin. Analysis of particle size (0.5-3.

Characterization of phosphate transporter genes and the function of SgPT1 involved in phosphate uptake in Stylosanthes guianensis.

Phosphorus (P) is one of the principal macronutrients for plant growth and productivity. Although the phosphate (Pi) transporter (PT) of the PHT1 family has been functionally characterized as participating in Pi uptake and transport in plants, information about PT genes in stylo (Stylosanthes guianensis), an important tropical forage legume that exhibits good adaptability to low-P acid soils, is limited. In this study, stylo root growth was found to be stimulated under P deficiency.