Analysis of chloroplast genome structure and phylogeny of the traditional medicinal of Ardisia crispa (Myrsinaceae).

Ardisia crispa(Myrsinaceae) is an ethnomedicine with horticultural and important medicinal values. Its morphology is complex, and its identification is difficult. We analyse the chloroplast genome characteristics and phylogenetic position of A.

Using multiomics to explore the weight differences between genders in Muscovy ducks.

Sexual dimorphism in poultry, especially in Muscovy ducks, is a proven phenomenon characterized by significant differences in body weight, growth patterns, and gene expression between male and female individuals. However, there is a dearth of research on the candidate genes and mechanisms underlying these weight differences. We selected 301 Muscovy ducks and recorded their weekly body weights from birth.

Co(II) Metal-Organic Complex: Fluorescence Performances and Loaded With Drug Vitamin B2 Hydrogels Against Recurrent Oral Ulcers.

A new coordination polymer (CP) based on Co(II), namely, {[Co(L)(4,4'-bipy)(DMA)]·HO} (1) has been synthesized after reacting Co(NO)·6HO with HL ligand in the existence of N-donor ligand 4,4'-bipyridine (4,4'-bipy), via utilizing a flexible tricarboxylic acid ligand 5-((formic acid-3-sulfur)methyl)isophthalic acid (HL) with -S-CH- joint. Additionally, the excellent blue fluorescence properties of CP 1 were confirmed through fluorescence spectroscopy compared to the original ligand. Using natural polysaccharide hyaluronic acid (HA) and carboxymethyl chitosan (CMCS) as raw materials, HA/CMCS hydrogel was prepared by chemical synthesis method.

Complete Genome Sequence of TH-1, a Candidate Biocontrol Bacterium from China.

TH-1 is a plant growth-promoting rhizobacteria with biocontrol potential that was isolated from the rhizosphere of Radix Our previous results showed that strain TH-1 demonstrated effective biocontrol activity against root rot of Radix and bacterial wilt of ginger. Currently, only a few whole-genome sequences of biocontrol strains isolated from the rhizosphere of medicinal plants are available. We report, here, the complete genome sequence of TH-1.

The complete chloroplast genome of Diels 1900, one traditional medicinal plant in southern China.

The complete chloroplast (cp) genome of Diels 1900, one traditionally medicinal plant usually used in southern China, was first assembled and reported in this study. The genome size is 156,742 bp (37.1% GC content), containing a large single-copy (LSC) region of 86,329 bp, a small single-copy (SSC) region of 18,417 bp, and a pair of inverted repeat regions (IRs) of 25,998 bp.

Enhancing ethylene glycol and ferric chloride pretreatment of rice straw by low-pressure carbon dioxide to improve enzymatic saccharification.

Ethylene glycol and ferric chloride pretreatment assisted by low-pressure carbon dioxide (1 MPa CO) realized the targeted deconstruction of lignocelluloses at 170 °C for 5 min, achieving 98 % cellulose recovery with removal of 92 % lignin and 90 % hemicellulose. After the pretreatment, the formation of stable platform mono-phenol components would be with the destruction of the lignin-carbohydrate complexes structure, and the surface of rice straw became rough, with a less negative charge and higher specific surface area, while the enzyme adsorption rate increased by 8.1 times.

Use of Microscopic Characteristics and Multielemental Fingerprinting Analysis to Trace Three Different Cultivation Modes of Medicinal and Edible Dendrobium officinale in China.

The traceability of different cultivation modes is critical for ensuring the commercial viability of high-value Dendrobium officinale. In this study, by means of polarizing microscopy, SEM-EDX, ICP-MS and ICP-AES, the possibility of combining microscopic characteristics, multielemental analysis and multivariate statistical authenticity analysis was realized to determine the origins of the fresh stem and dried stem powder of D. officinale derived from three different cultivation modes from six provinces of China.

The complete chloroplast genome of B. L. Guo et Hsiao (Berberidaceae).

The genus of belongs to Berberidaceae family, which is famous for their medicinal and ornamental value. In recent years, has attracted increasing attention due to their medicinal and nutritive value. In this research, we reported the complete chloroplast (cp) genome of The complete chloroplast of this species is 157,076 bp in length, including a pair of invert repeat regions (IRS) (25,833 bp) that is divided by a large single copy area (LSC) (88,340 bp) and a small single copy area (SSC) (17,070 bp).

[Effects of light quality on growth and icariin flavonoid content of Epimedium pseudowushanense under different light intensity].

In this study, the growth index including plant height, compound leaf area, specific leaf area, leaf water content, number of branches, and leaf biomass per plant and the icariin flavonoids such as epimedin A, epimedin B, epimedin C and icariin of Epimedium pseudowushanense were determined on 30 d and 60 d under light intensity(18.2±2.5) μmol·m~(-2)·s~(-1)(L1) and(90.

The Soluble and Particulate Form of Alginates Positively Regulate Immune Response.

Background: Alginate materials have been widely employed for biomedical applications ranging from wound healing to cancer treatment. However, how alginate materials affect the immune system is largely unknown.

Objective: To explore the impact of alginate materials on immune system.

Alginate Particles with Ovalbumin (OVA) Peptide Can Serve as a Carrier and Adjuvant for Immune Therapy in B16-OVA Cancer Model.

BACKGROUND Alginate is a natural polysaccharide obtained from brown algae and has been shown to have numerous applications in biomedical science, such as wound healing, delivery of bioactive agents, and cell transplantation. Ovalbumin (OVA) peptide 323-339 has been reported to be involved in immune response.  MATERIAL AND METHODS This work investigated the use of alginate particles as a carrier and adjuvant for the immune therapy of cancer.

[Study on HPLC fingerprint of miao medicine Ardisia japonica].

Objective: To establish the HPLC fingerprint of Ardisia japonica.

Methods: HPLC analysis was performed on a Diamonsil C18 column (250 mm x 4.6 mm, 5 microm) with the eluting system of gradient consisted of methanol-0.

[Study on sequence characterized amplified region (SCAR) markers of Polygonum capitatum].

Objective: To establish sequence characterized amplified region markers of Polygonum capitatum.

Method: The random primer was screened through RAPD to obtain the specific RAPD marker band, and the band was separated, extracted, cloned and sequenced. The specific primers were designed for conventional PCR reaction on the basis of the specific band, and the SCAR marker was acquired.

Development and validation of HPLC coupled with triple quadrupole MS for the simultaneous determination of six phenolic acids, six flavonoids, and a lignan in Polygonum capitatum.

Polygonum capitatum, a traditional Miao medicinal plant, has significant effects on the treatment of urinary system infections and pyelonephritis. However, no study about the comprehensive quality evaluation of P. capitatum has been reported.

Aspergillus niger DLFCC-90 rhamnoside hydrolase, a new type of flavonoid glycoside hydrolase.

A novel rutin-α-L-rhamnosidase hydrolyzing α-L-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the α-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13.

Enhancement of the differentiation of protocorm-like bodies of Dendrobium officinale to shoots by ultrasound treatment.

An efficient micropropagation protocol has been developed for Dendrobium officinale, through protocorm-like bodies (PLBs). A correlation between enhanced differentiation of PLBs of D. officinale by ultrasound and changes in the levels of endogenous hormones and the antioxidant enzyme activities was described.

[Analysis on chloroplast DNA sequences of Polygonum capitatum of different geographical population].

Objective: To investigate the relationship between the variation of chloroplast DNA gene sequences and the geographical origins of Polygonum capitatum in order to provide the molecular evidence for its excellent germplasm resources.

Method: PCR direct sequencing was applied to detect the chloroplast psbA-trnH, trnL-trnF gene sequence of 11 samples collected from 11 populations of P. capitatum.

[Genetic diversity of Polygonum capitatum from Guizhou populations by ISSR markers].

Objective: To detect genetic diversity of 48 population of Polygonum capitatum in Guizhou province.

Method: The genetic diversity of 48 representational populations of P. capitatum including 240 individuals had been investigated by ISSR marker technique.

Nanoleakage expression and microshear bond strength in the resin cement/dentin interface.

Purpose: The aim of this study was to evaluate dentin sealing ability and microshear bond strength of two different resin cements.

Materials And Methods: Resin composite overlays (Estenia C&B) were fabricated and cemented to mid-dentin surfaces with either a self-adhesive resin cement (RelyX Unicem) or a resin cement with a self-etching primer (Panavia F2.0).

Effect of double-application of three single-step self-etch adhesives on dentin bonding and mechanical properties of resin-dentin area.

Purpose: This study investigated whether double-application influences the bond strength of single-step self-etch adhesives and the mechanical properties of the resin-dentin area.

Materials And Methods: Three single-step self-etch adhesives (EXL-683, experimental, 3M ESPE; Clearfil Tri-S Bond, Kuraray Medical; G BOND, GC) were applied on dentin surfaces by using the single-application or double-application method. The manufacturers' instructions stated use of the single-application method.

Effect of caries-affected dentin hardness on the shear bond strength of current adhesives.

Purpose: The aim of this study was to evaluate the microshear bond strength of three current adhesives to normal and caries-affected dentin, and to examine the correlation between dentin nanoindentation hardness and bond strength.

Materials And Methods: Nanoindentation hardness of dentin and microshear bond strength of Clearfil SE Bond, Clerafil Tri-S Bond (Kuraray Medical) and Single Bond (3M ESPE) were measured on caries-affected and normal dentin. Modes of fracture for the bonding tests and the resin/dentin interfaces were observed using SEM.

Production of Gluconobacter oxydans cells from low-cost culture medium for conversion of glycerol to dihydroxyacetone.

Gluconobacter oxydans could be immobilized as a biocatalyst for the conversion of glycerol to dihydroxyacetone. To reduce the production cost, the cells were produced from agricultural byproducts. Corn meal hydrolysate and corn steep liquor were employed to replace of sorbitol and yeast extract as medium for G.

Repeated use of immobilized Gluconobacter oxydans cells for conversion of glycerol to dihydroxyacetone.

Dihydroxyacetone (DHA) is of great interest in the fine chemical and pharmaceutical industry; therefore, the discovery of suitable biocatalysts for the efficient production of it is very necessary. In the experiment, Gluconobacter oxydans was immobilized in polyvinyl alcohol (PVA). Various parameters of the immobilized cells were investigated.