Disruption of manganese ions [Mn(II)] transporter genes DR1709 or DR2523 in extremely radio-resistant bacterium Deinococcus radiodurans.

Objective: The primary objective of this study was to identify whether the manganese ions [Mn(II)] transporter genes DR1709 and DR2523 played roles in Deinococcus radiodurans's radiation resistance. The second objective was to study the relationships among manganese ions, manganese ions transporter genes and the bacterial radioresistance.

Methods: We constructed mutants of DR1709 and DR2523.

[Construction of the gene drb0099 deleted mutant and adversity analysis in the extremely radioresistant bacterium Deinococcus radiodurans].

Deinococcus radiodurans R1 has extraordinary resistance to radiation. DRB0099 might play an important role in protecting the bacterium against radiation. To verify the inference, we deleted drb0099 and constructed the mutant.