Sensitive phenotyping of serum extracellular vesicles on a SERS-microfluidic platform for early-stage clinical diagnosis of ovarian carcinoma.

Ovarian carcinoma (OvCa) poses a severe threat to women's health due to its high mortality rate and lack of efficient early diagnosis approach. There is evidence to suggest that nanosized small extracellular vesicles (sEVs) which carrying cell-specific components from OvCa can serve as potential diagnostic biomarkers. Herein, we reported a Surface-enhanced Raman Scattering (SERS)-multichannel microchip for sEVs (S-MMEV) assay to investigate the phenotype changes of sEVs.

Fluorescence labeling of extracellular vesicles for diverse bio-applications and .

Extracellular vesicles (EVs) are nanosized vesicles enclosed in a lipid membrane that are sustainably released by nearly all cell types. EVs have been deemed as valuable biomarkers for diagnostics and effective drug carriers, owing to the physiological function of transporting biomolecules for intercellular communication. To investigate their biological properties, efficient labeling strategies have been constructed for EV research, among which fluorescence labeling exerts a powerful function due to the capability of visualizing the nanovesicles with high sensitivity both and .

Protective Effects of (Burm.f.) Lindau Aqueous Extract on HBV Mouse Model by Modulating Gut Microbiota and Liver Metabolomics.

Background: (Burm.f.) Lindau () has been used in the therapy of hepatitis B (HB) and is effective; however, the mechanism of action has not been elucidated.

Miq. Extract Prevents Diabetes in Mice by Modulating Gut Microbiota.

Recently, the role of gut microbiota in the development of obesity and type 2 diabetes mellitus (T2DM) has been highlighted. We performed an 8-week administration protocol on T2DM (C57BL/6J db-/db-) mice and fecal samples were collected. Comparisons of fecal bacterial communities were performed between db-/db- mice and normal mice (DB/DB) and between the db-/db mice treated and untreated with AOE using next-generation sequencing technology.

MicroRNA-374b inhibits liver cancer progression via down regulating programmed cell death-1 expression on cytokine-induced killer cells.

Programmed cell death-1 (PD-1) is an oncogene associated with suppressing proliferation and cytokine production of T cells in the progression of liver cancer. microRNAs (miRs) regulate gene expression via specific binding to the target 3'untranslated region of mRNA. In the present study, miR-374b was indicated to interact with PD-1 and affect the tumor-targeting capacity of cytokine-induced killer (CIK) cells.

Anti-diabetic effect of extract on 57BL/KsJ db-/db- mice.

Diabetes mellitus is characterized by high blood glucose levels. Increased levels of reactive oxygen species (ROS) may disrupt insulin signaling and result in insulin resistance. The extract (AOE) possesses powerful antioxidant activity and may therefore inhibit the development of insulin resistance.

Alpinia oxyphylla Miq. extract changes miRNA expression profiles in db-/db- mouse kidney.

Background: A number of dysregulated miRNAs have been identified and are proposed to have significant roles in the pathogenesis of type 2 diabetes mellitus or renal pathology. Alpinia oxyphylla has shown significant anti-inflammatory properties and play an anti-diabetes role. The objective of this study was to detect the alteration of miRNAs underlying the anti-diabetes effects of A.

Effects of Porphyromonas gingivalis extracellular vesicles on human periodontal ligament fibroblasts.

The purpose of this study was to investigate the influencing mechanism of Porphyromonas gingivalis extracellular vesicles on human periodontal ligament fibroblasts to better understand the pathogenesis of periodontitis, the major cause of adult tooth loss. Human periodontal ligament fibroblasts were cultured and randomly assigned to a control group and an extracellular vesicles (ECV) group. The ECV group was exposed to isolated Porphyromonas gingivalis extracellular vesicles; the control group was not exposed.