Rice, a staple in diets, undergoes digestion post-consumption, often triggering a swift surge in blood sugar among diabetics, intensifying their health burden. Notably, resistant starch (RS) emerges as a potent ally in fostering satiety and mitigating metabolic syndrome in diabetes. The SBEIIb gene, a key orchestrator of starch branching enzymes, plays a pivotal role in starch synthesis, and its genetic alteration can dramatically boost RS content in rice.
View Article and Find Full Text PDFThe plant hormone auxin controls many aspects of plant development. Membrane trafficking processes, such as secretion, endocytosis and recycling, regulate the polar localization of auxin transporters in order to establish an auxin concentration gradient. Here, we investigate the function of the Arabidopsis thaliana R-SNAREs VESICLE-ASSOCIATED MEMBRANE PROTEIN 721 (VAMP721) and VAMP722 in the post-Golgi trafficking required for proper auxin distribution and seedling growth.
View Article and Find Full Text PDFGlycoprotein (GP) 2a was a minor structural protein of porcine reproductive and respiratory syndrome virus (PRRSV) and was one of crucial proteins for PRRSV to bind cell receptor, which indicated that there were neutralizing epitopes on GP2a. In the present work, we used mouse anti-GP2a serum and one GP2a specific monoclonal antibody (McAb) to identify B-cell epitopes of GP2a by peptide-based ELISA. A liner B-cell epitope FPTPGSRPKLHDFQQ was identified.
View Article and Find Full Text PDFis an important food-borne pathogen that can persist in food processing environments and thus contaminate food products. Benzalkonium chloride (BC) is a common disinfectant widely used in food industry. Selective pressure associated with exposure to BC may result in adaptation to this agent in .
View Article and Find Full Text PDFFront Microbiol
September 2018
Efflux is a primary fluoroquinolone resistance mechanism in . In the present study, ciprofloxacin resistant strains were selected by exposure of sensitive strain to progressively increasing concentrations of ciprofloxacin and then the roles of efflux pumps Lde and MdrL in the development of resistance to ciprofloxacin were also investigated in . Ciprofloxacin sensitive strain of exhibited reduced susceptibility to this antibiotic after induction.
View Article and Find Full Text PDFIn this study, efflux pump-mediated benzalkonium chloride (BC) resistance, including plasmid-encoded (Qac protein family and BcrABC) and chromosome-borne efflux pumps, was investigated in Listeria monocytogenes from retail food in China. Among the 59 L. monocytogenes strains, 13 (22.
View Article and Find Full Text PDFThe aim of this study was to investigate the presence of extended-spectrum β-lactamase (ESBL) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolated from retail meat samples in Henan Province, China. E. coli isolates were detected in 179 of 645 (27.
View Article and Find Full Text PDFBackground: On the basis of comprehending several membrane proteins undergoing ectodomain shedding, including tumor necrosis factor (TNF) alpha receptors, we want to know if Fas (CD95/APO-1), which belongs to the TNF receptor (TNFR) superfamily and transduces signals resulting in apoptosis upon binding of Fas ligand (L) or agonistic anti-Fas antibody, is shed on its ectodomain during liver regeneration
Methods: After purification of the total membrane protein of hepatocytes, we analyzed Fas ectodomain shedding, using Western blotting, and determined the effect of Fas shedding on the apoptosis of hepatocytes by a statistical method relevant to apoptosis.
Results: Fas protein ectodomain shedding occurred at 2, 12, and 36 h after partial hepatectomy, and its level decreased at 2 months after partial hepatectomy. The same results were gained from in-vitro cultured hepatocytes induced by serum from rats after partial hepatectomy.