[Study on determination of bovine serum albumin using o-hydroxyphenylfluorone reagent with fluorescence spectrophotometry].

The reaction conditions for the determination of bovine serum albumin (BSA) using o-hydroxyphenylfluorone (o-HPF) as fluorescence probe reagent were studied. In the B-R medium at pH 7.90, o-HPF could react with BSA, producing a stable complex compound which resulted in fluorescent quenching of this binary system.

[Study on the determination of bovine serum albumin using o-hydroxyphenylfluorone reagent with fluorescence spectrophotometry].

The reactions were studied for the determination of bovine serum albumin (BSA) using o-hydroxyphenylfluorone (o-HPF) as fluorescence probe reagent with fluorescence spectrophotometry. In the B-R medium, o-HPF reacts with BSA to form the stable complex compound. The reaction system of o-HPF and BSA was binary system, and the method for the determination of BSA was of good selectivity and stability and simplicity.

[Stability of upper molars with the application of implant anchorage].

Objective: To investigate the stability of upper molars with the application of micro-screw implant anchorage during orthodontic treatment.

Methods: Fifteen adult patients with severe maxillary protrusion were included. Upper first premolars were extracted and upper posterior anchorage was reinforced with micro-screw implant in all patients.