[Detection of Mycoplasma pneumoniae, Chlamydia trachomatis and common respiratory viruses in children with acute respiratory infection in Nanjing].

Objective: To investigate the nonbacterial pathogens in children with acute respiratory infection (ARI) in Nanjing.

Methods: The presence of Mycoplasma pneumoniae (MP) and Chlamydia trachomatis (CT) was determined by quantitative PCR in the nasopharyngeal samples from 1 592 hospitalized children with ARI. Common respiratory viruses, including respiratory syncytial virus (RSV), adenovirus (ADV), influenza virus types A and B (IVA and IVB), parainfluenza virus types 1, 2, 3(PIV-1, 2, 3) and human metapneumovirus (hMPV), were detected using direct immunofluorescence assay.

[The correlation factor about respiratory syncytial virus bronchiolitis and post-bronchiolitis wheezing in infant].

Objective: To observe the correlation factor about early-life RSV bronchiolitis and sequential recurrent wheezing for two years.

Methods: Follow up the RSV bronchiolitis patients for two years in order to analyze the occurrence of wheezing post-bronchiolitis. Single and multiple logistic regression analysis were used to determined the risk factors such as individual atopy history and familial atopy history, pet feeding, breast milk, secondhand smoke for RSV bronchiolitis and subsequent wheezing.

[Association of RANTES gene promoter -28C/G polymorphism with respiratory syncytial virus bronchiolitis].

Objective: Respiratory syncytial virus (RSV) infects nearly all children under two years of age. It is poorly understood why a few children who were infected with RSV develop bronchiolitis that require hospital admission while most have a relatively minor illness. Several recent studies have obtained some indications for the involvement of genetic heterogeneity in RSV bronchiolitis, implying that the clinical outcome of RSV infection perhaps is determined by genetic factors.

Low level of galacto-oligosaccharide in infant formula stimulates growth of intestinal Bifidobacteria and Lactobacilli.

Aim: To investigate the effect of a new infant formula supplemented with a low level (0.24 g/100 mL) of galacto-oligosaccharide (GOS) on intestinal micro-flora (Bifidobacteria, Lactobacilli and E. coli) and fermentation characteristics in term infants, compared with human milk and a standard infant formula without GOS.

Effect of variation in RANTES promoter on serum RANTES levels and risk of recurrent wheezing after RSV bronchiolitis in children from Han, Southern China.

To investigate the association among RANTES (regulated on activation normal T cell expressed and secreted) gene promoter polymorphism, serum RANTES levels, and recurrent wheezing after RSV (respiratory syncytial virus) bronchiolitis in children (1-12 months of age) from Han, Southern china. Three hundred twenty children with RSV bronchiolitis and 272 controls were enrolled in the 3-year follow-up study. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP), enzyme-linked immunosorbent assay (ELISA) kit and luciferase analysis were the mainly used methods, which were used to genotype the RANTES (-403 G/A), assess the serum RANTES levels and the RANTES promoter activity.

[Association between interleukin-8 gene-251 locus polymorphism and respiratory syncytial virus bronchiolitis and post-bronchiolitis wheezing in infants].

Objective: Respiratory syncytial virus (RSV) infects nearly all children under two years of age. It is not understood why some develop serious bronchiolitis. Whether there is a genetic component is not known.