Evaluating the efficacy of MEANGS for mitochondrial genome assembly of cartilaginous and ray-finned fish species.

The assembly of complete and circularized mitochondrial genomes (mitogenomes) is essential for population genetics, phylogenetics and evolution studies. Recently, Song et al. developed a seed-free tool called MEANGS for de novo mitochondrial assembly from whole genome sequencing (WGS) data in animals, achieving highly accurate and intact assemblies.

Management of regional citrate anticoagulation for continuous renal replacement therapy: guideline recommendations from Chinese emergency medical doctor consensus.

Continuous renal replacement therapy (CRRT) is widely used for treating critically-ill patients in the emergency department in China. Anticoagulant therapy is needed to prevent clotting in the extracorporeal circulation during CRRT. Regional citrate anticoagulation (RCA) has been shown to potentially be safer and more effective and is now recommended as the preferred anticoagulant method for CRRT.

Three-Dimensional Encoding Approach for Wearable Tactile Communication Devices.

In this work, we presented a novel encoding method for tactile communication. This approach was based on several tactile sensory characteristics of human skin at different body parts, such as the head and neck, where location coordinates in the three-dimensional (3D) space were clearly mapped in the brain cortex, and gentle stimulations of vibrational touching with varied strengths were received instantly and precisely. For certain applications, such as playing cards or navigating walk paths for blinded people, we demonstrated specifically designed code lists with different patterns of tactile points in varied temporal sequences.

Development of an intensive simulating training program in emergency medicine for medical students in China.

Background: A national standardized emergency medicine (EM) curriculum for medical students, including specific competencies in procedural skills, are absent in many countries. The development of an intensive simulating training program in EM, based on a tight schedule, is anticipated to enhance the competency of medical students.

Methods: A 3-day intensive EM training program, consisting of four procedural skills and 8-hour case-based learning (CBL), was developed by experienced physicians from the EM department in Peking Union Medical College Hospital (PUMCH).

Chromosome-scale genome assembly of brown-spotted flathead sp.1 provides insights into demersal adaptation in flathead fish.

Flatheads are valuable commercial fish species endemic to the Indo-West Pacific. Due to their economic value and unique biological traits, such as metamorphosis and camouflage, they serve as ideal marine organisms for studies on demersal adaptation and evolution. The brown-spotted flathead ( sp.

Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19).

Background: The emergence of coronavirus disease 2019 (COVID-19) is a major healthcare threat. The current method of detection involves a quantitative polymerase chain reaction (qPCR)-based technique, which identifies the viral nucleic acids when present in sufficient quantity. False-negative results can be achieved and failure to quarantine the infected patient would be a major setback in containing the viral transmission.

Whole genome survey analysis and microsatellite motif identification of Sebastiscus marmoratus.

The marbled rockfish Sebastiscus marmoratus is an ecologically and economically important marine fish species distributed along the northwestern Pacific coast from Japan to the Philippines. Here, next-generation sequencing was used to generate a whole genome survey dataset to provide fundamental information of its genome and develop genome-wide microsatellite markers for S. marmoratus.

The Effects of Natural Chinese Medicine Aconite Root, Dried Ginger Rhizome, and on Rectal and Skin Temperatures at Acupuncture Points.

The 4 properties of Chinese refer to cold, hot, warm, and cool. In the present study, the effects of the , the prepared aconite root, and dried ginger rhizome were compared with regard to the rectal and skin temperature changes of the related body surface acupuncture points (Dazhui, Zhiyang, Mingmen, Zhongwan, and Shenque). The investigation aimed to explore the thermal sensitive points, which can reflect the cold and hot properties of the Chinese herbs.

Imaging of soft material with carbon nanotube tip using near-field scanning microwave microscopy.

In this manuscript, a near-field scanning microwave microscope (NSMM) of our own design is introduced while using a multi-walled carbon nanotube (MWCNT) bundle as the tip (referred to as 'CNT tip'). Clear images of gold-patterned numbers, photoresist stripes and corneal endothelial cells (cell line B4G12) were obtained by mapping the resonant frequency fr and S11 amplitude of a given area while the NSMM is operating in tapping mode. The CNT tip helps to improve image quality and reveals more information about the sample as compared to a traditional metallic tip.

One-atmosphere aqueous-solution synthesis of trimolybdate nanomaterials and the feasibility for mass production.

Mass production of low-cost functional nanomaterials is an important issue for the development of nanoscience and nanotechnology. With rich structural, physical and chemical properties, polyoxometalates are important functional materials for both industrial applications and fundamental research. We presented a family of alkali trimolybdate nanowires and nanorods that were synthesized by a one-atmosphere aqueous solution method from a mixture of two solutions, one consisting of (NH4)6Mo7O24 x 4H2O and the other of Li+, Na+, K+ and Rb+ ions, respectively.

Thermoelectric measurement of multi-walled carbon nanotube bundles by using nano-probes.

We report here a method for measurement of thermoelectric power of quasi-one dimensional nano-materials with a simple platform, where individual nanomaterial is assembled with nano-probes in a scanning electron microscope. This approach allows repeated manipulation and thermoelectric measurement of the same loaded nanosample with adjustable number of individual nanotubes or nanowires. It also allows assembly of multiple samples on one measurement stage.

Transmission electron microscope observation of a freestanding nanocrystal in a Coulomb potential well.

Usually pure electrostatic charges cannot be utilized alone to form macro-sized three-dimensional electromagnetic traps for trapping charged particles. These traps need additional dynamic electromagnetic fields. We report here the observation of a CdSe nanocrystal floating over a carbon film in a transmission electron microscope.