Design of Mn-based nanozymes with multiple enzyme-like activities for identification/quantification of glyphosate and green transformation of organophosphorus.

A Mn-based nanozyme, Mn-uNF/Si, with excellent alkali phosphatase-like activity was designed by in-situ growth of ultrathin Mn-MOF on the surface of silicon spheres, and implemented as an effective solid Lewis-Brønsted acid catalyst for broad-spectrum dephosphorylation. HO-mediated GC-MS studies confirmed the cleavage sites and the involvement of HO in the new bonds. DRIFT NH-IR and in-situ ATR-FTIR confirmed the coexistence of Lewis-Brønsted acid sites and the adjustment of adsorption configurations at the interfacial sites.

Design of bifunctional ultrathin MnO nanofilm with laccase-like activity for sensing environmental pollutants containing phenol groups.

Laccase-catalyzed oxidative reactions are increasingly examined as a reliable approach to environmental analysis and remediation, and it is urgent to widen metal category to compensate huge gap in the number of studies on copper- and non-copper laccase mimics. Herein, two-dimensional ultrathin MnO nanofilm (Mn-uNF) was designed via a chemical deposition and alkali etching process. Similar to Cu-laccase, Mn-uNF can oxidize phenols via a one-electron-transfer reaction of Mn(III) and accelerate the Mn/Mn state cycle through an unconventional oxygen reduction process.

Design and Engineering of an Efficient Peroxidase Using Myoglobin for Dye Decolorization and Lignin Bioconversion.

The treatment of environmental pollutants such as synthetic dyes and lignin has received much attention, especially for biotechnological treatments using both native and artificial metalloenzymes. In this study, we designed and engineered an efficient peroxidase using the O carrier myoglobin (Mb) as a protein scaffold by four mutations (F43Y/T67R/P88W/F138W), which combines the key structural features of natural peroxidases such as the presence of a conserved His-Arg pair and Tyr/Trp residues close to the heme active center. Kinetic studies revealed that the quadruple mutant exhibits considerably enhanced peroxidase activity, with the catalytic efficiency (/) comparable to that of the most efficient natural enzyme, horseradish peroxidase (HRP).

Self-compassion and the Mitigation of Negative Affect in the Era of Social Distancing.

Objectives: The outbreak of contagious diseases and their associated non-pharmaceutical interventions can lead to negative mental health consequences. This study aimed to investigate online self-compassion exercises' effectiveness in alleviating people's negative affect (anxiety and negative emotions) during the COVID-19 pandemic lockdown.

Methods: Study 1 examined the associations between self-compassion and negative affect using an anonymous online survey.